Method of preparing hydrogen by using xylose as substrate for fermentation

Abstract

The invention relates to a method of preparing hydrogen by using xylose as a substrate for fermentation.The method particularly includes: adopting an xylose solution as the substrate and anaerobic digestion sludge, Clostridium butyricum INET1 or Enterococcus faecium INET2 as hydrogen-generating strains, wherein the xylose solution is an xylose water solution or cellulose hydrolyzate; fermenting under anaerobic conditions to prepare hydrogen, wherein maximum hydrogen yield of xylose is 105-330 mL / 100mL and hydrogen generating efficiency of each gram of xylose is 66-228 mL.By implementing the method, the problem that the cellulose hydrolyzate cannot be fully utilized due to the fact that xylose is difficult to be utilized by microorganisms is solved effectively.Xylose is a main product of plant cellulose, and plant fiber raw materials are rich in resource, low in price and easy to get; xylose which is an important hydrolyzate of plant cellulose is used as the substrate for biological hydrogen generation, so that raw material cost of biological fermentation for hydrogen generation can be lowered and full utilization of cellulose raw materials can be realized.

Description

technical field [0001] The invention belongs to the technical field of hydrogen production by biological fermentation, and in particular relates to a method for preparing hydrogen by fermenting xylose as a substrate. Background technique [0002] Hydrogen energy is a secondary energy source. The current hydrogen production processes mainly include water electrolysis hydrogen production, pyrolysis hydrogen production, photochemical hydrogen production, nuclear energy hydrogen production, fossil fuel hydrogen production and biological hydrogen production. Hydrogen production by electrolysis of water is currently widely used and relatively mature. The efficiency of hydrogen production by electrolysis of water is generally 75-85%. The process is simple and pollution-free, but the power consumption is large, so its application is limited. Photochemical hydrogen production is a method of producing hydrogen by photocatalytic decomposition of water as raw material. Hydrogen produc...

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Problems solved by technology

For example, studies have found that some thermophilic hydrogen-producing strains can use xylose in cellulose hydrolyzate for growth, but there is no report on the hydrogen production efficiency of using xylose (Zhu Yuhong. High-temperature fermentation hydrogen-producing strain W16 and its Hydrogen production characteristics of fermentation [D]. Harbin Institute of Technology, 2008; Liu Yan. Thermoanaerobic Clostridium degradation of cellulose and hydrogen production characteristics [D]. Shandong University, 2008.); and Niu Kun's research found that, Xylose in cellulose hydrolyzate will inhibit its hydrogen production effect by affecting the metabolic pathway of microorganisms (Niu Kun. Analysis of dark fermentation hydrogen production process of Klebsiellapneumoniae ECU-15 strain and experimental research on using lignocellulose hydrolyzate[D]. East China University of Science and Technology, 2010.)