Method of preparing hydrogen by using xylose as substrate for fermentation

A xylose and substrate technology, applied in the field of biological fermentation hydrogen production, can solve the problems of unreported hydrogen production efficiency, achieve the effects of improving utilization rate, expanding the scope of substrates, and realizing reduction

Active Publication Date: 2016-07-13
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

For example, studies have found that some thermophilic hydrogen-producing strains can use xylose in cellulose hydrolyzate for growth, but there is no report on the hydrogen production efficiency of using xylose (Zhu Yuhong. High-temperature fermentation hydrogen-producing strain W16 and its Hydrogen production characteristics of fermentation [D]. Harbin Institute of Technology, 2008; Liu Yan. Thermoanaerobic Clostridium degradation of cellulose and h...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Using xylose as a substrate to inoculate irradiated pretreated anaerobic digested sludge for hydrogen production by fermentation:

[0024] The anaerobic digested sludge was irradiated with 5kGy gamma rays for pretreatment to 60 Co as a radioactive source. The pre-irradiated anaerobic digested sludge was enriched and pre-cultured, and the pre-culture medium components were: xylose 20g / L, peptone 10g / L, yeast powder 0.5g / L, and nutrient solution 10mL / 100mL. After pre-cultivation, inoculate 10mL into 100mL fermentation hydrogen production medium, the composition of fermentation hydrogen production medium is: xylose 50g / L, nutrient solution 10mL / 100mL, use 5mol / L HCl solution or 5mol / L NaOH Adjust the initial pH of the solution to 7.0, blow off nitrogen to test the air tightness and remove the oxygen in the bottle, place the reaction bottle in a constant temperature water bath shaker, ferment and produce hydrogen at 35°C, 100r / min, and record the gas production at interval...

Embodiment 2

[0026] Using xylose as a substrate to inoculate irradiated pretreated anaerobic digested sludge for hydrogen production by fermentation:

[0027] The anaerobic digested sludge was irradiated with 5kGy gamma rays for pretreatment to 60 Co as a radioactive source. The pre-irradiated anaerobic digested sludge was enriched and pre-cultured, and the pre-culture medium components were: xylose 20g / L, peptone 10g / L, yeast powder 0.5g / L, and nutrient solution 10mL / 100mL. After pre-cultivation, take 10mL and inoculate it into 100mL fermentation hydrogen production medium. The composition of fermentation hydrogen production medium is: xylose 5g / L, nutrient solution 10mL / 100mL, use 5mol / L HCl solution or 5mol / L NaOH Adjust the initial pH of the solution to 7.0, blow off nitrogen to test the air tightness and remove the oxygen in the bottle, place the reaction bottle in a constant temperature water bath shaker, ferment and produce hydrogen at 35°C, 100r / min, and record the gas production ...

Embodiment 3

[0029] Using xylose as a substrate, inoculate Clostridium butyricum INET1 to ferment hydrogen production:

[0030]Pick the Clostridium butyricum INET1 colony from the plate medium, inoculate it in 200mL enrichment culture medium for enrichment pre-culture, the enrichment medium composition is as follows: glucose 50g / L, peptone 10g / L, yeast powder 0.5g / L L, nutrient solution 10mL / 100mL. After 8-10 hours of growth, the strain enters the logarithmic phase. At this time, take 10mL of the bacterial liquid in the logarithmic phase and inoculate it into 100mL fermentation hydrogen production medium. The composition of the fermentation hydrogen production medium is: xylose 10g / L, nutrient Liquid 10mL / 100mL, use 5mol / L HCl solution or 5mol / L NaOH solution to adjust the initial pH to 7.0, use nitrogen to blow off to test air tightness and get rid of oxygen in the bottle, place the reaction bottle on a constant temperature water bath shaker, Hydrogen was produced by fermentation at 35°C...

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Abstract

The invention relates to a method of preparing hydrogen by using xylose as a substrate for fermentation.The method particularly includes: adopting an xylose solution as the substrate and anaerobic digestion sludge, Clostridium butyricum INET1 or Enterococcus faecium INET2 as hydrogen-generating strains, wherein the xylose solution is an xylose water solution or cellulose hydrolyzate; fermenting under anaerobic conditions to prepare hydrogen, wherein maximum hydrogen yield of xylose is 105-330 mL/100mL and hydrogen generating efficiency of each gram of xylose is 66-228 mL.By implementing the method, the problem that the cellulose hydrolyzate cannot be fully utilized due to the fact that xylose is difficult to be utilized by microorganisms is solved effectively.Xylose is a main product of plant cellulose, and plant fiber raw materials are rich in resource, low in price and easy to get; xylose which is an important hydrolyzate of plant cellulose is used as the substrate for biological hydrogen generation, so that raw material cost of biological fermentation for hydrogen generation can be lowered and full utilization of cellulose raw materials can be realized.

Description

technical field [0001] The invention belongs to the technical field of hydrogen production by biological fermentation, and in particular relates to a method for preparing hydrogen by fermenting xylose as a substrate. Background technique [0002] Hydrogen energy is a secondary energy source. The current hydrogen production processes mainly include water electrolysis hydrogen production, pyrolysis hydrogen production, photochemical hydrogen production, nuclear energy hydrogen production, fossil fuel hydrogen production and biological hydrogen production. Hydrogen production by electrolysis of water is currently widely used and relatively mature. The efficiency of hydrogen production by electrolysis of water is generally 75-85%. The process is simple and pollution-free, but the power consumption is large, so its application is limited. Photochemical hydrogen production is a method of producing hydrogen by photocatalytic decomposition of water as raw material. Hydrogen produc...

Claims

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Application Information

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IPC IPC(8): C12P3/00C12N13/00C12R1/125C12R1/46
Inventor 王建龙阴亚楠
Owner TSINGHUA UNIV
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