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A method for hybrid breeding of Amorphophallus verruca

A technology of Amorphophallus verrucosum and hybrid breeding, which is applied in botany equipment and methods, plant gene improvement, application, etc. It can solve the problems of short flowering period, flowering is not fruitful, and the effect is not exerted, so as to achieve low cost and less virus accumulation. , the effect of high seed setting rate

Active Publication Date: 2018-10-02
DEHONG DAI JINGPO AUTONOMOUS PREFECTURE AGRI TECH PROMOTION CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Amorphophallus verrucosum described in the present invention has the advantages of resistance to konjac soft rot and high yield, and is a very rare excellent germplasm resource in the improvement of konjac varieties. The fruit is strong, which has led to the current academic mistakenly believe that Amorphophallus verrucosum is "flowers but not fruit", so that its role in Amorphophallus konjac hybrid breeding has not been brought into play

Method used

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  • A method for hybrid breeding of Amorphophallus verruca
  • A method for hybrid breeding of Amorphophallus verruca
  • A method for hybrid breeding of Amorphophallus verruca

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Experimental program
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Effect test

Embodiment 1

[0046] In 2010, 26 flowering plants of Amorphophallus verruca were bred according to technical step A (the same below) in the experimental field of Dehong Prefecture Agricultural Science Institute; according to technical step E (the same below), assisted artificial pollination was carried out, and 1 plant was successfully induced to bear fruit. ~6 weeks later, the scape was as high as 91cm, and the appendages were separated from the mother body, and the growth was normal in the later period; the remaining 25 plants turned yellow and withered after 2 weeks of pollination, and the appendages remained; in late December, the berries of the surviving plants were fully mature and red. Harvest the seeds in batches according to the technical step F (the same below), and air-dry them on the spot; a total of 418 berries are harvested, the thousand-grain weight of the berries is 286g, and each berry contains 1-2 seeds. In March of the following year, seedlings were sown according to techn...

Embodiment 2

[0048] In 2011, 21 flowering plants of Amorphophallus verrucosum were cultivated; the pollen vigor was measured according to the technical step (B, the same below); the cross-pollination was carried out according to the technical step (C1), and the pollination time was between 19:00 and 21:00 (the following The same), respectively numbered observation records (see Table 1); after artificial pollination, 4 plants became fruitful, and the material numbers were: 4, 5, 8, 20; the remaining plants turned yellow and withered after 2 weeks; in late December, the surviving plants The berries were mature and the appendages were persistent. The seeds were harvested in batches and air-dried on the spot. A total of 1412 berries were harvested, with a thousand-grain weight of 289g (see Table 1). In March of the following year, 2125 seedlings were planted and 2125 seedlings were obtained; in mid-May, the field was transplanted, and the survival rate reached 88.3%; in mid-November, the plants...

Embodiment 3

[0052] In 2012, 57 flowering plants of Amorphophallus verruca were bred. Measure the pollen viability according to the technical step (B); the first batch of flowering plants did not bear fruit after being pollinated by the same flower; select 40 plants to carry out the artificial assisted pollination experiment according to the technical step (C1), and set the bracts to be scattered 60%, 80%, and 100% ("Trumpet") and loose powder stage four treatments, each treatment 10 plants. The experimental results are shown in Table 2, fruit setting rate = number of plump fruits / number of seeds. The test results showed that the same pollination of Amorphophallus verrucoides did not bear fruit; the bract leaves unfolded for 1d to 2d, and the seed setting rate was the highest; by the pollination stage, the female flowers had completely lost their vigor and could not bear fruit.

[0053] Table 2 Morphological characteristics of Amorphophallus konjac bracts and hybrid seed setting rate

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Abstract

The invention discloses a method for amorphophallus virosus crossbreeding. The method comprises the following technical steps: cultivating flowering plants, measuring the vitality of pollens, preparing female flower maturity judgment criteria, performing pollination, seed harvesting and preservation, seedling transplanting, and the like. According to the flowering habit of amorphophallus virosus, by the application of technical measures such as reasonable selection of flowering seed taros, measurement of the vitality of the pollens, accurate mastering of the time node of female flower maturity, artificial auxiliary pollination at proper time and scientific seed harvesting and preservation, artificially hybridized seeds can be obtained continuously for many years, and the hybridization success rate is 90 percent or above; the first-generation seed taros have the characteristics of low virus accumulation and high yield. According to the method for amorphophallus virosus crossbreeding, the difficulty of non fruiting of amorphophallus virosus under a natural condition is solved, and a foundation is laid for development and application of high-quality amorphophallus virosus seed resources; a new hope is brought to solve the world problem of soft rot diseases of amorphophallus virosus; the method has the advantages of being simple in operation, low in cost, high in fruiting rate and the like.

Description

technical field [0001] The invention belongs to the technical field of plant seed cultivation, and in particular relates to a method for hybrid breeding of Amorphophallus verruca. Background technique [0002] Konjac ( Amorphophallus konjac ) belonging to the family Araceae ( Areas ) Konjac ( Amorphophallus ) plants, konjac is a kind of economic crop with high value for food, medicine, health care and industrial use. Konjac has pharmacological effects from konjac glucomannan (KGM) extracted from its tubers. Gel, cohesive, and low-calorie edible properties are widely used in food processing, daily chemicals, and health products. However, konjac has serious diseases, low reproductive coefficient of seed taro, and high production risk. Soft rot is a devastating disease in konjac production, known as "konjac cancer". At present, there is no effective control agent, which has restricted the development of konjac industry for a long time. Amorphophallus verrucosum described...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02
CPCA01H1/02
Inventor 徐文果郑宇峰黄廷祥张晓梅岩所张国云张磊车宏志赵庆云陈军文黄炜李佳芹李兴清王艳谢世清
Owner DEHONG DAI JINGPO AUTONOMOUS PREFECTURE AGRI TECH PROMOTION CENT
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