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A method for treating petroleum sewage

A technology of sewage and PetroChina, which is applied in the direction of chemical instruments and methods, biological water/sewage treatment, water/sludge/sewage treatment, etc. It can solve the problems of expensive equipment costs, low effect, harmful chemical extraction agents, etc., and achieve cost reduction , save time, good application prospects

Active Publication Date: 2019-07-02
广东创晟控股集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, chemical extraction agents are mostly harmful to aquatic ecological species, and their side effects are more serious than the direct economic losses caused by the flooding of oil pollution. Therefore, many countries have banned the use of this method
The physical method is to absorb and collect oil pollution through some equipment or additives. Many types of such devices have been developed, and the removal effect is good, but the expensive equipment costs limit its wide use, and the effect is low in cold seasons

Method used

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  • A method for treating petroleum sewage
  • A method for treating petroleum sewage
  • A method for treating petroleum sewage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1 removes the method for petroleum in sewage

[0017] Take oil-polluted water bodies, where the initial content of total petroleum hydrocarbons is 9000mg / L.

[0018] (1) introduce the water body to be treated into the treatment tank; (2) mix Rhodococcus erythropolis and Pseudomonas aeruginosa with mixed bacteria in a ratio of 1:1, and insert the bacterial liquid into the sewage, and the inoculation amount is 2.0×10 9 ~3.5×10 9 1 / L sewage, then add 1000~2000mg / kg biosurfactant, add nutrient salt nitrogen source and phosphorus source; degrade at 30°C for 35 days. After 35 days, the total petroleum hydrocarbon content was measured to be 483mg / L, and the degradation efficiency was 94.7%.

Embodiment 2

[0020] According to the carrier construction and engineering bacteria construction methods disclosed in CN 103333848B, primers were designed using DNAMAN software, enzyme cutting sites were added, and the nucleotide sequence AJW gene was synthesized according to the amino acid sequence Genbank: AJW87477.1 whole gene, passed Amplify to obtain the target fragment, PCR amplification to obtain the target gene AJW (simultaneously introduce the corresponding mutation site into the gene sequence through multiple PCR, thereby obtaining different mutant genes), carry out double digestion with restriction endonuclease The PCR product is connected with the shuttle expression plasmid pRES1 that has also undergone double enzyme digestion, and the successfully verified recombinant plasmid is transformed into the Rhodococcus erythroflates to obtain a genetically engineered bacterium that degrades petroleum. Respectively named as Rhodococcus 31g / s, 62d / k Rhodococcus, 130i / g Rhodococcus, 165h / g...

Embodiment 3

[0021] Embodiment 3 Verification of the treatment effect of the petroleum-containing sewage of Rhodococcus erythropolis engineering bacteria

[0022] According to the method of Example 1, the corresponding sewage treatment experiment was carried out, and the sewage and the sewage of Example 1 belonged to the same batch and had the same concentration of oil. Wherein, the Rhodococcus erythroflates prepared in Example 1 was replaced by the Rhodococcus erythroflates prepared in Example 2, and the composition of the remaining components was exactly the same as that in Example 1.

[0023] Through experiments, it was found that 31g / s Rhodococcus, 62d / k Rhodococcus, 130i / g Rhodococcus, 165h / g Rhodococcus, 182a / s Rhodococcus, 244d / a Rhodococcus Rhodococcus, 263s / r Rhodococcus, 271g / p Rhodococcus, 279i / v Rhodococcus, 302e / s Rhodococcus, 365l / m Rhodococcus, 372k / g Rhodococcus, 383a / s Rhodococcus, 409k / a Rhodococcus, 419w / t Rhodococcus, 423e / v Rhodococcus all had significantly enhanced e...

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Abstract

The invention belongs to the field of sewage treatment and discloses a method for removing petroleum in sewage. The method comprises the following steps of proportionally mixing rhodococcus erythropolis and pseudomonas aeruginosa with mixed bacteria; adding a nutritive salt nitrogen source and a phosphorus source so as to degrade the petroleum. According to the method disclosed by the invention, microorganisms are mainly adopted for treating pollutants, so that pollutants containing the petroleum can be effectively removed, furthermore the invested cost is low, petroleum degradation is rapid, and the method has relatively good application prospect.

Description

technical field [0001] The invention belongs to the technical field of environmental protection, and in particular relates to a method for removing petroleum in petroleum sewage. Background technique [0002] Petroleum is one of the main energy sources in modern society, known as "industrial blood". Petroleum is a complex mixture composed of thousands of substances with different chemical properties, mainly including saturated hydrocarbons, aromatic hydrocarbons, asphaltene, resins, etc. Now there are more than 150 oil-producing countries and regions, and more than 40,000 oil and gas fields have been discovered. Among them, my country has found more than 400 oil-producing countries with an annual output of nearly 200 million tons. It is one of the top ten oil-producing countries in the world. However, in the process of oil extraction, smelting and transportation, sudden accidents, leakage or improper operation, as well as the discharge of oily wastewater, sewage irrigation...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/21C02F3/34C07K14/32
CPCC02F3/34C07K14/32
Inventor 汪晓明
Owner 广东创晟控股集团有限公司