Blueberry small molecular peptide composition, as well as extracting method and application thereof

A technology of small molecular peptide and extraction method, applied in the field of food processing, can solve the problems of wide molecular weight distribution, peculiar smell, unfavorableness, etc., and achieve the effects of promoting blood vessel expansion and elasticity, preventing blood vessel rupture, and saving production costs.

Inactive Publication Date: 2016-07-20
蔡庭守
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the blueberry preparations obtained by these methods contain a large number of unnecessary substances, and even have a special smell. In addition, the purity of the active ingredients is still not enough, especially the protein usually retains almost complete macromolecular forms
The bioavailability of proteins in the form of macromolecules is generally low, which is unfavorable in the health care and cosmetic industries
[0004] Therefore, people need to hydrolyze protein into small peptides through various hydrolysis methods to improve its bioavailability. The methods of proteolysis mainly include acid method, alkali method and enzymatic method, and the acid method and alkali method have

Method used

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  • Blueberry small molecular peptide composition, as well as extracting method and application thereof

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Effect test

Embodiment 1

[0042] A method for extracting a blueberry small molecule peptide composition, comprising the following steps:

[0043] (1) Raw material pretreatment: put the blueberry fruit in a drying oven at 55°C for 15 hours, and crush it into 100-mesh blueberry powder for later use;

[0044] (2) Denaturation of blueberry protein: mix the blueberry powder with deionized water at a ratio of 1:10, then heat to 93°C and keep it warm for 15 minutes until the protein is denatured, and then cool down to 48°C at a rate of 48°C / min ℃, obtained liquid;

[0045] (3) Compound enzymatic hydrolysis of blueberry protein:

[0046] Regulate the pH of the feed solution to 8 with a NaOH solution with a molar concentration of 10% mol / L, add alkaline protease with an enzyme activity of 500,000 u / g relative to the weight of the blueberry powder, and insulate for enzymolysis 2h, get the first proteolysis solution;

[0047] Adjust the pH of the first proteolysis solution to 7.2 with a HCl solution with a mol...

Embodiment 2

[0054] A method for extracting a blueberry small molecule peptide composition, comprising the following steps:

[0055] (1) Raw material pretreatment: put the blueberry fruit in a drying oven at 58°C for 15.5 hours, and crush it into 100-mesh blueberry powder for later use;

[0056] (2) Denaturation of blueberry protein: mix the blueberry powder and deionized water at a ratio of 1:12, then heat to 94°C and keep it warm for 18 minutes until the protein is denatured. ℃, obtained liquid;

[0057] (3) Compound enzymatic hydrolysis of blueberry protein:

[0058] Adjust the pH of the feed liquid to 8.5 with a NaOH solution with a molar concentration of 10% mol / L, add alkaline protease with an enzyme activity of 1% relative to the weight of the blueberry powder and an enzyme activity of 500,000 u / g, and insulate for enzymolysis 3h, get the first proteolysis solution;

[0059] Adjust the pH of the first proteolysis solution to 7.3 with a HCl solution with a molar concentration of 1...

Embodiment 3

[0066] A method for extracting a blueberry small molecule peptide composition, comprising the following steps:

[0067] (1) Raw material pretreatment: put the blueberry fruit in a drying oven at 60°C for 16 hours, and crush it into 100-120 mesh blueberry powder for later use;

[0068] (2) Denaturation of blueberry protein: mix the blueberry powder and deionized water at a ratio of 1:15, then heat it to 95°C and keep it warm for 20 minutes until the protein is denatured. ℃, obtained liquid;

[0069] (3) Compound enzymatic hydrolysis of blueberry protein:

[0070] Regulate the pH of the feed solution to 9 with a NaOH solution with a molar concentration of 10% mol / L, add alkaline protease with an enzyme activity of 500,000 u / g relative to the weight of the blueberry powder, and insulate for enzymolysis 2.5h, get the first proteolysis solution;

[0071] Adjust the pH of the first proteolysis solution to 7.5 with a HCl solution with a molar concentration of 10% mol / L, and add ne...

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Abstract

The invention relates to an extracting method of a blueberry small molecular peptide composition. The method comprises the following steps: (1) pretreatment of raw materials; (2) modification of blueberry protein; (3) composite enzymolysis of blueberry protein; (4) filtration and purification; and (5) dehydration and drying. The blueberry small molecular peptide composition contains small molecular peptide obtained through blueberry protein hydrolysis and provided with molecular weight mainly distributed between 100-700 D, and further comprises chlorogenic acid, citrate, arbutin, myricetin, flavonoids, vitamins and trace-elements, and can be applied to medical raw materials, healthcare food and cosmetics. The molecular weight of the produced obtained through the invention is mainly distributed between 100-700D, the obtained small molecular peptide usually is dipeptide, tripeptide, tetrapeptide or other small molecular structure easily absorbed and utilized by a human body, and the small molecular peptide with molecular weight distributed at 60-700 occupies over 90% of the total weight of the protein.

Description

technical field [0001] The present invention relates to the technical field of food processing, in particular to a method for extracting a small molecule peptide composition from blueberries, the small molecule peptide composition extracted by the method and the use of the small molecule peptide composition in pharmaceutical raw materials and health food , Application in beauty cosmetics. Background technique [0002] Blueberry is also called Blueberry, Dusi, Dushi, Duji, Dushi, Dianguo, Dusi Bilberry, Latin scientific name (VacciniumSpp), its fruit contains a variety of nutrients, in addition to conventional sugar, acid and vitamin C In addition, it is rich in protein, vitamin E, vitamin A, vitamin B, superoxide dismutase (SOD), arbutin, anthocyanin, dietary fiber and rich in potassium, iron, zinc, calcium and other mineral elements. Higher than other fruits, trace elements are also very high, which is a fruit high in amino acids, high copper, high zinc, and high iron. In...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/36C07K1/34C07K1/14A23L33/18A61K38/01A61K36/45A61K8/64A61P27/02A61P27/06A61P27/12A61P9/14A61P7/02A61P9/10
CPCC12P21/06A61K8/64A61K36/45A61K38/011C07K1/14C07K1/34C07K1/36
Inventor 蔡庭守
Owner 蔡庭守
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