SNP (Single Nucleotide Polymorphism) marker for identifying dendrobium huoshanense C.Z. Tang et S.J.Cheng and molecular detection method for SNP marker

Abstract

The invention discloses an SNP (Single Nucleotide Polymorphism) marker for identifying dendrobium huoshanense C.Z. Tang et S.J.Cheng and a molecular detection method for the SNP marker, and provides application of SNP of the following SNP site in a dendrobium genome or a substance for detecting the SNP of the following SNP site in the dendrobium genome to identification or auxiliary identification of dendrobium. The SNP site in the dendrobium genome corresponds to the 113th site of a nucleotide sequence shown in sequence 3 in a sequence table; a nucleotide at the SNP site is T or G. According to the SNP marker and the molecular detection method, sequencing analysis is performed on 27 kinds of dendrobium samples comprising all species of dendrobium huoshanense C.Z. Tang et S.J.Cheng complexes to find the functional SNP molecular marker only existing in the dendrobium huoshanense C.Z. Tang et S.J.Cheng by virtue of an RAD (Restriction Site Associated) technology, and the detection method for the SNP marker is established to accurately identify the dendrobium huoshanense C.Z. Tang et S.J.Cheng and other dendrobium (comprising hybrids of the dendrobium huoshanense C.Z. Tang et S.J.Cheng and the other dendrobium).

Description

technical field [0001] The invention belongs to the field of molecular identification of traditional Chinese medicines, and relates to a SNP marker for identifying Dendrobium Huoshanense and a molecular detection method thereof, in particular to a SNP marker for identifying Dendrobium Huoshanense and a PCR-RFLP-based kit for identifying Dendrobium Huoshanense and identification methods. Background technique [0002] The medicinal material Dendrobium huoshanense is derived from the fresh or dried stems of Dendrobiumhuoshanense C.Z.TangetS.J.Cheng. Dendrobium Huoshanense is sweet, sticky to the teeth, and chews without residue. It is the top grade of Dendrobium medicinal materials. It is expensive and has been out of stock for a long time. At present, some Fengdou products with names such as "Huoshan Dendrobium", "Huo Dou" or "Wild Golden Huoshan Dendrobium" in the domestic and foreign markets are often not processed from genuine Huoshan Dendrobium, and the production of coun...

AI Technical Summary

Problems solved by technology

However, the latest molecular phylogenetic theory shows that Dendrobium Huoshanense belongs to the complex of D. 6 species including .okinawense and D. xichouense (XiangXGetal, Molecular Phylogenetics and Evolution, 2013, 69:950-960), the species in the complex cannot be distinguished by trait-independent markers

On the other hand, ISSR-PCR and site-specific PCR techniques can only detect whether the tested sample contains the original D.

However, the hybrid varieties of Dendrobium Huoshanense and other Dendrobium cannot be used as medicinal materials of Dendrobium Huoshanense.

[0004] Dendrobium Huoshanense, a commodity circulating in the market, has changed in shape after processing, transportation and storage, making it difficult to distinguish

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