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Digital microscope and focusing method thereof

A technology of digital microscope and focusing method, applied in the field of microscopy, can solve problems such as affecting the automatic focusing time and results of microscopes, image over-exposed lines, and insufficient

Active Publication Date: 2016-07-20
TAGYE TECH HANGZHOU CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This will lead to overexposure or insufficient light in the image of the measured result when the microscope is moving the stage, both of which will affect the autofocus time and results of the microscope

Method used

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  • Digital microscope and focusing method thereof
  • Digital microscope and focusing method thereof
  • Digital microscope and focusing method thereof

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Experimental program
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Effect test

Embodiment 1

[0098] For the first technical problem to be solved by the present invention, this embodiment adopts the implementation mode as follows:

[0099] like figure 1 As shown, the traditional microscope has a fixed image distance v and a fixed focal length f. According to the following formula 1, the object distance u is also fixed. Only by moving the stage to "find" the object distance u to achieve focus. The difference between this embodiment and the traditional microscope is that when the working distance is constant, the main control unit inside the microscope controls the movement of the focusing lens group in the microscope to "find" a suitable image distance v to achieve automatic focusing , please refer to figure 2 , which makes the optical imaging formulation from the conventional

[0100] 1 f = 1 u + 1 v Formula 1

[0101] becomes

[01...

specific Embodiment

[0125] Figure 9 It shows the method of automatic focusing of digital microscope under the condition of fixed working distance. In this method, real-time acquisition of the original image of the target to be measured through the focusing lens group 22b to obtain multiple sets of image data through the image data processing unit 246a, multiple sets of image data through the focus value calculation unit 246b to obtain multiple sets of focus values, The focus algorithm unit 247c selects an appropriate value as the focus evaluation value based on multiple sets of focus values ​​and thresholds. After two stages of coarse focus and fine focus, different step lengths are used in different stages to control the focus speed and precision of the motor. In the coarse focus stage, through the analysis of the focus value, take a large step to quickly focus, find the range of the best focus value, and drive the focusing lens group 22b to move to a certain reference position within the range...

Embodiment 2

[0174] The embodiment that the second technical problem to be solved by the present invention takes is as follows:

[0175] In traditional microscopes, since the working distance is fixed, if the height of the object to be measured changes, the image will be unclear, and it is necessary to readjust the stage or the main body of the microscope to re-image clearly, and the movement of the stage The range is limited. When the target of this kind of microscope is large and the size exceeds its controllable range, it will lead to the inability to achieve correct focus no matter how the stage moves; in addition, because the size of the measured object is unknown in advance , often leads to too long time of focusing or focusing.

[0176] like image 3 and Figure 4 As shown, this embodiment is different from the traditional microscope in that the working distance w is variable. According to the optical imaging formula 3, f is fixed, the image distance v is variable (from v to v'), ...

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Abstract

The invention relates to a digital microscope and a focusing method thereof. According to the digital microscope and the focusing method thereof of the invention, a focusing lens group, a zoom lens group and corresponding control circuits are integrated in a microscope; a main control unit in the microscope executes a focusing search algorithm and a focusing tracking algorithm so as to control the focusing lens group and the zoom lens group to move; and the microscope is made to realize automatic focusing through adjusting an image distance; and the microscope is made to realize zoom through changing a focal length. With the digital microscope and the focusing method thereof of the invention adopted, automatic focusing can be realized without moving an object stage and the microscope, and focusing efficiency is improved, and focusing time is shortened; and a zoom tracking algorithm is adopted, so that continuous zoom and tracking can be realized, and images are clear in a whole zoom process. According to the digital microscope and the focusing method thereof of the invention, an exposure algorithm is executed through the main control unit, and the brightness of an auxiliary light source at the front end of the microscope can be automatically adjusted through the change of luminous flux, and therefore, uniform exposure can be realized.

Description

technical field [0001] The invention relates to the technical field of microscopes, in particular to a digital microscope and a focusing method thereof. Background technique [0002] The focus of traditional microscopes mostly adopts the method of manual focus, which completely depends on human subjective judgment, which makes the focus efficiency low, especially in the case of a large number of targets, it is easy to cause visual fatigue and reduce the focus on The focus accuracy of the target object is easy to cause boredom. [0003] Existing auto-focus microscopes mostly control the movement of the stage or the microscope body to find the set object distance to achieve auto-focus, such as figure 1 As shown, the adjustable range of this method is limited, and when the object to be measured is large, the bracket of the microscope needs to be designed to be very long in order to achieve correct focus, which is not conducive to manual observation and use. [0004] The worki...

Claims

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Application Information

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IPC IPC(8): G02B21/24G02B7/36
Inventor 徐加军楼品琪龚晔
Owner TAGYE TECH HANGZHOU CO LTD
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