Debaryomyces hansenii bacterial strain and method for preparing 3-hydroxypropionic acid by fermenting debaryomyces hansenii bacterial strain

A technology of yeast strain and hydroxypropionic acid, applied in microorganism-based methods, biochemical equipment and methods, fermentation and other directions, can solve the problems of few natural bacteria and low yield, and achieve short production cycle, fast reproduction and wide application prospects Effect

Active Publication Date: 2016-08-17
SHANGHAI CHANGING BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few natural bacteria that can

Method used

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  • Debaryomyces hansenii bacterial strain and method for preparing 3-hydroxypropionic acid by fermenting debaryomyces hansenii bacterial strain
  • Debaryomyces hansenii bacterial strain and method for preparing 3-hydroxypropionic acid by fermenting debaryomyces hansenii bacterial strain
  • Debaryomyces hansenii bacterial strain and method for preparing 3-hydroxypropionic acid by fermenting debaryomyces hansenii bacterial strain

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Experimental program
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Effect test

Embodiment 1

[0031] Example 1: Isolation, screening and purification of Debaryomyces hansenii. IS451 strain

[0032] Sample collection of Debaryomyces hansenii. IS451 strain: It is mainly isolated from soil collected from orchards and human feces samples obtained.

[0033] The screening and separation method is as follows: a small amount of soil collected from the orchard and a small amount of human feces obtained were soaked in sterile saline, and left to stand for 12 hours; after 12 hours, the bacterial suspension was serially diluted, and the concentration of the gradient dilution was followed by 10 -4 , 10 -5 , 10 -6 , 10 -7 , 10 -8 , 10 -9 ; Diluted bacterial suspension, to be transferred;

[0034] Prepare screening media with propionic acid content (V / V) of 0.4%, 0.8%, 1.2%, 1.6%, and 2.0%, and adjust the pH value to 6.0 with a pH meter. After sterilization, pour the plate, and screen each plate for culture The amount of the base is controlled at 10 mL, and the screening plate...

Embodiment 2

[0044] Embodiment 2: Under optimal fermentation condition, Debaryomyces hansenii.IS451 bacterial strain high yield 3-hydroxy propionic acid situation

[0045] The method for preparing 3-hydroxypropionic acid by fermentation of the Debaryomyces hansenii strain is prepared as follows:

[0046] Step 1) Inoculate the Debaryomyces hansenii. IS451 strain in the slant medium for activation, culture at a constant temperature of 30°C for 24 hours, take 2 rings of the slant IS451 strain, and use the inoculation loop to inoculate the slant strain twice ;

[0047] The components and their dosages in the slant medium are: glucose 20.00 g / L, yeast extract 10.00 g / L, peptone 20.00 g / L, agar powder 20.00 g / L.

[0048] Then insert the seed medium, the volume of the medium is 50mL / 250mL, cultivate in a constant temperature oscillator, the rotation speed is 180 r / min, and cultivate at 30°C for 48h, and cultivate to the logarithmic phase to obtain the IS451 strain seed liquid;

[0049] Each com...

Embodiment 3

[0055] Embodiment 3: under general fermentation condition, Debaryomyces hansenii.IS451 bacterial strain produces 3-hydroxy propionic acid situation

[0056] The method for preparing 3-hydroxypropionic acid by fermentation of the Debaryomyces hansenii strain is prepared as follows:

[0057] Step 1) Inoculate the Debaryomyces hansenii. IS451 strain in the slant medium for activation, culture at a constant temperature of 30°C for 24 hours, take 2 rings of the slant IS451 strain, and use the inoculation loop to inoculate the slant strain twice ;

[0058] The components and their dosages in the slant medium are: glucose 20.00 g / L, yeast extract 10.00 g / L, peptone 20.00 g / L, agar powder 20.00 g / L.

[0059] Then insert the seed medium, the volume of the medium is 50mL / 250mL, cultivate in a constant temperature oscillator, the rotation speed is 180 r / min, and cultivate at 30°C for 48h, and cultivate to the logarithmic phase to obtain the IS451 strain seed liquid;

[0060] Each compo...

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Abstract

The invention discloses a Debaryomyces hansenii bacterial strain. The Debaryomyces hansenii bacterial strain is Debaryomyces hansenii. IS451, and the preservation number is CGMCC No.11893. A method for preparing 3-hydroxypropionic acid by fermenting the bacterial strain comprises the following steps that an agar slant culture medium is inoculated with the IS451 bacterial strain to activate the bacterial strain, then a seed medium is inoculated with the activated IS451 bacterial strain, the IS451 bacterial strain is cultured in a constant-temperature oscillator to a logarithmic phase, and IS451 bacterial strain seed liquid is prepared; a fermentation culture medium is inoculated with the cultured IS451 bacterial strain seed liquid according to the volume ratio of 10%, and the pH is regulated to 6.0; the IS451 bacterial strain seed liquid is cultured in the constant-temperature oscillator, fermenting is conducted for 5 days, and then fermentation liquid is prepared; it is detected that the content of 3-hydroxypropionic acid in the fermentation liquid reaches 48.96 g/L and is three times or above higher than that of a method for producing 3-hydroxypropionic acid through microbial fermentation in literature reports, and the wide application prospect is achieved.

Description

technical field [0001] The invention relates to a Debaria hansenii strain and a method for fermenting and preparing 3-hydroxypropionic acid, belonging to the fields of biochemical engineering and fermentation engineering. Background technique [0002] Affected by world oil resources, prices, environmental protection, and global climate change, the development of bio-based resources has become an important measure for many countries to improve resource and energy security, reduce greenhouse gas emissions, and address climate change. 3-hydroxypropionic acid (3-hp) is an important chemical intermediate that has emerged in recent years. 3-hp has two functional groups, hydroxyl and carboxyl, and is the precursor of many optically active substances. It is an important platform compound , its output directly affects the production of many high value-added chemicals. 3-hp can be used as a raw material for the production of malonic acid, 1,3-propanediol (PDO), succinic acid, special...

Claims

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Application Information

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IPC IPC(8): C12N1/16C12P7/42C12R1/645
CPCC12P7/42C12N1/145C12R2001/645
Inventor 王陶储渊明李文董玉玮高明侠张传丽陈宏伟
Owner SHANGHAI CHANGING BIOTECH CO LTD
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