Rice variety Lengshuihong Laodelphax striatellus antibiotic locus and molecular marking method thereof
A technology of molecular marker and SBPH, applied in the field of agricultural biology, can solve the problems of time-consuming, labor-intensive, poor repeatability, and speed up the breeding process of rice SBPH-resistant varieties, and achieve the effects of clear location, speeding up the breeding process, and convenient identification.
Inactive Publication Date: 2016-08-17
王纪芝
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Problems solved by technology
[0007] The purpose of the present invention is to aim at the shortcomings of the stability, repeatability and time-consuming and labor-intensive identification of SBPH in conventional rice breeding, and to provide a rice variety cold-water red striatellus antibiotic site and its molecular marker method. The resistance of rice plants to SBPH can be predicted, the selection method is simplified, and the breeding process of rice SBPH resistant varieties can be accelerated
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[0028] The present invention will be further described below in conjunction with embodiment case, rather than limit the present invention, the method used in the embodiment is all conventional technical methods unless otherwise specified.
[0029] 1. Screening of resistance sources and construction of Wuyujing 3 / Lengshuihong F2 population:
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Abstract
The invention discloses a Laodelphax striatellus antibiotic locus derived from rice variety Lengshuihong and a molecular marking method thereof, belonging to the technical field of agricultural biology. The Laodelphax striatellus antibiotic locus is named Qsbph4a, and is located between molecular markers RM1018 and RM7187 of rice chromosome 4. The molecular marker RM1018 primer or molecular marker RM7187 primer is utilized to amplify the rice Laodelphax striatellus resistance identification material, and if amplification segments 160bp and 157bp can be respectively obtained, the Laodelphax striatellus antibiotic locus Qsbph4a exists in the rice material. When being used for detecting the rice Laodelphax striatellus resistance level, the Laodelphax striatellus antibiotic locus and molecular marking method thereof can greatly increase the Laodelphax striatellus antibiotic rice detection speed and enhance the rice yield and breeding efficiency.
Description
technical field [0001] The invention relates to a rice variety cold water red gray planthopper antibiotic locus and a molecular marking method thereof, and belongs to the field of agricultural biotechnology. Background technique [0002] Rice ( Oryza sativa L. ) is one of the most important food crops in the world, and its output and sown area both rank first in the world. Rice pests and diseases are important factors restricting the high and stable yield of rice. Rice planthopper is one of the most important pests in rice production in Asian countries, causing serious damage to rice production. At present, the planthoppers that damage rice mainly include the brown planthopper ( Nilaparvata lugens ), Strigophyllus striatellus ( Laodelphax striatellus ) and white-backed planthopper ( Sogatella furcifera ) three types, among which SBPH occurs the earliest, has the widest adaptability, and has a large population density in the field. [0003] Strigophyllus stri...
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IPC IPC(8): C12Q1/68
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 王纪芝
Owner 王纪芝
