Emission type optical projection tomography attenuation and scattering correction method

Abstract

The invention discloses an emission type optical projection tomography (OPT) attenuation and scattering correction method. An OPT model under the condition of weak scattering and an OPT fluorescence reconstruction algorithm are combined. OPT fluorescence signal attenuation correction is conducted using a normalized Born ratio method; the scattering component in measured data is simulated using the Monte Carlo technology, and the scattering component is removed from detection data; and through an iterative framework effectively combining attenuation correction and scattering component estimation, quantitative 3D reconstruction of OPT fluorescent dye concentration is realized. The influence of both absorption and scattering is considered comprehensively in quantitative reconstruction of fluorescent dye concentration, the scattering component in signals is estimated using the Monte Carlo technology and removed, and quantitative reconstruction of fluorescence dye concentration is realized.

Description

technical field [0001] The invention belongs to the technical field of medical image processing, in particular to a transmission optical projection tomography attenuation and scattering correction method. Background technique [0002] Optical projection tomography (OPT) is a new type of high-resolution three-dimensional molecular imaging technology, and its imaging principle is similar to that of X-ray computed tomography. OPT uses visible light as the irradiation source, and is very suitable for imaging transparent or translucent samples in the range of 1-10mm, such as small animal embryos, organs, fruit flies, and nematodes. OPT technology allows people to obtain high-definition three-dimensional structural images without losing the integrity of biological tissues and organs, and can achieve molecular-specific imaging through fluorescent labeling technology. OPT has the characteristics of dual-modal fusion, which just meets the needs of the biomedical research field to us...

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide an emission optical projection tomography attenuation and scattering correction method for weakly scattering samples, aiming at solving the problem that the traditional emission OPT reconstruction method does not consider the influence of absorption effect on the attenuation caused by the fluorescence signal, and the Inaccurate Quantitative Reconstruction of Fluorescent Signals Caused by Weak Scattering

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