Odor-resistant and antibacterial pad attached with probiotics and production method and application of odor-resistant and antibacterial pad
An antibacterial pad and deodorant technology, applied in the field of daily necessities, can solve the problems of the normal life of the family, reduce the air quality of the living environment, etc., to prevent the probability of tinea pedis, reduce the requirements of storage conditions and transportation conditions, and enhance the function of Effect
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Embodiment 1
[0048] A preparation method of a deodorant and antibacterial pad for adsorbing probiotics, comprising the steps of:
[0049] (1) Take Bacillus subtilis, activate and cultivate it at 37°C for 18h, then ferment and cultivate it at 37°C for 30h, to obtain a Bacillus subtilis fermentation broth with a viable bacterial concentration of 11 billion / ml and a sporulation rate of more than 95%, and centrifuge at 5000rpm 20min, get precipitation, make Bacillus subtilis;
[0050] The above-mentioned Bacillus subtilis activation medium has the following components, all in mass percent:
[0051] Glucose 1.5%, peptone 0.3%, yeast powder 0.5%, sodium chloride 0.1%, balance water, pH 6.8.
[0052] The above-mentioned Bacillus subtilis fermentation medium has the following components, all in mass percent:
[0053] Glucose 2%, peptone 0.1%, corn steep liquor 0.5%, yeast extract 0.4%, corn flour 0.5%, dextrin 0.6%, potassium dihydrogen phosphate 0.1%, magnesium sulfate 0.01%, potassium chloride...
Embodiment 2
[0064] A preparation method of a deodorant and antibacterial pad for adsorbing probiotics, comprising the steps of:
[0065] (1) Take Bacillus subtilis, activate and cultivate it at 37°C for 20h, then ferment and cultivate it at 37°C for 28h, to obtain a Bacillus subtilis fermentation broth with a viable bacterial concentration of 10.5 billion / ml and a sporulation rate of more than 95%, and centrifuge at 5000rpm 20min, get precipitation, make Bacillus subtilis;
[0066] The above-mentioned Bacillus subtilis activation medium has the following components, all in mass percent:
[0067] Glucose 1%, peptone 0.5%, yeast powder 0.8%, sodium chloride 0.3%, balance water, pH7.2;
[0068] The above-mentioned Bacillus subtilis fermentation medium has the following components, all in mass percent:
[0069] Glucose 3%, peptone 0.2%, corn steep liquor 0.6%, yeast extract 0.5%, corn flour 0.8%, dextrin 0.3%, potassium dihydrogen phosphate 0.2%, magnesium sulfate 0.03%, potassium chloride ...
Embodiment 3
[0080] A preparation method of a deodorant and antibacterial pad for adsorbing probiotics, comprising the steps of:
[0081] (1) Take Bacillus subtilis, activate and cultivate it at 37°C for 22h, then ferment and cultivate it at 37°C for 30h, to obtain a Bacillus subtilis fermentation broth with a viable bacterial concentration of 14 billion / ml and a spore formation rate of more than 95%, and centrifuge at 5000rpm 20min, get precipitation, make Bacillus subtilis;
[0082] The above-mentioned Bacillus subtilis activation medium has the following components, all in mass percent:
[0083] Glucose 1.8%, peptone 0.5%, yeast powder 0.7%, sodium chloride 0.15%, balance water, pH7.0;
[0084] The above-mentioned Bacillus subtilis fermentation medium has the following components, all in mass percent:
[0085]Glucose 2.5%, yeast powder 0.5%, corn steep liquor 0.8%, corn flour 0.5%, potassium dihydrogen phosphate 0.3%, magnesium sulfate 0.01%, sodium chloride 0.1%, manganese sulfate 0....
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