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Ningxia wolfberry carbon assimilation type determining method

A Ningxia Lycium barbarum, determination method technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of low photosynthetic efficiency, high compensation point, high photorespiration of three-carbon plants, etc., and achieve the effect of simple operation

Inactive Publication Date: 2016-09-21
NINGXIA UNIVERSITY
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  • Abstract
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Problems solved by technology

Three-carbon plants have high photorespiration, high carbon dioxide compensation point, and low photosynthetic efficiency
However, most crops currently use the C3 pathway and their photosynthetic efficiency is low, so it is of great significance to determine the carbon assimilation type of Ningxia Lycium barbarum for follow-up research

Method used

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Embodiment Construction

[0036] The following clearly and completely describes the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only some of the embodiments of the present invention, but not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0037] The present invention provides a technical solution: a method for determining the carbon assimilation type of Ningxia wolfberry, comprising the following steps:

[0038] A. Carry out 1,5-bisphosphate ribulose carboxylase and phosphoenolpyruvate carboxylase activity assays with Amarina and Ningxia Lycium barbarum;

[0039] B. Carry out stable carbon isotope determination of Amarina and Ningxia Lycium barbarum;

[0040] C. Comparing the anatomical structure of the leaves of Amarina tetraptera and Lycium barbarum Ningxia.

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Abstract

The invention discloses a Ningxia wolfberry carbon assimilation type determining method. The method comprises the following steps that A, atyipiex canexcens and Ningxia wolfberry are subjected to 1,5-ribulose diphosphate carboxylase and phosphoenolpyruvate carboxylase activity determination; B, atyipiex canexcens and Ningxia wolfberry are subjected to stable carbon isotope testing; C, anatomical structures of the atyipiex canexcens and the Ningxia wolfberry are compared. The method is easy to operate, the Ningxia wolfberry carbon assimilation type if determined through the leaf anatomical structures, carbon assimilation carboxylase activity and carbon isotope resolution ratio changes and other aspects, and a fundamental basis is provided for research of Ningxia wolfberry in the future.

Description

technical field [0001] The invention relates to the technical field of carbon assimilation type determination, in particular to a method for determining the carbon assimilation type of Ningxia wolfberry. Background technique [0002] So far, although Lycium barbarum is wild all over the world, as an economic plant resource, wild domestication, large-scale cultivation and comprehensive development and utilization are unique to China and have a long history. In the long process of human development, evolution and demand, the role and status of wolfberry as a "medicine and food dual-purpose" economic crop has also been continuously improved with the deepening of human understanding of it. It has experienced wild utilization, artificial domestication, and proper cultivation development process. The fruit of Ningxia wolfberry is rich in a variety of nutrients and trace elements, and has high nutritional value and medicinal health effects. Because wolfberry is resistant to drough...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/00
CPCG01N33/0098
Inventor 毛桂莲张晶张学萍马乐许兴王开元王盛
Owner NINGXIA UNIVERSITY
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