Method for quickly detecting nucleic acid of H5 subtype highly pathogenic avian influenza virus

Abstract

The invention belongs to the technical field of biology and determines a method for quickly detecting nucleic acid of H5 subtype highly pathogenic avian influenza virus. The method comprises three technical points: determining a primer sequence required for nucleic acid detection; determining detection reaction types; determining a detection reaction system and reaction conditions. The method is useful in scientific research of H5 subtype avian influenza virus and clinical diagnostic detection for animals or humans.

Description

technical field [0001] The invention belongs to the field of biotechnology; specifically, the invention establishes a rapid detection method for H5 subtype highly pathogenic avian influenza virus, which is used for the rapid detection of H5 subtype avian influenza virus, and is used in in vitro diagnosis, veterinary medicine, and food safety. , Biosafety and other fields have greater use value. Background technique [0002] According to different pathogenicity, avian influenza can be divided into highly pathogenic avian influenza, low pathogenic avian influenza and apathogenic avian influenza. Among them, highly pathogenic avian influenza is a disease caused by certain strains of H5 and H7 subtypes. [0003] Viral nucleic acid detection is one of the important methods for virus detection. At present, various nucleic acid detection methods for H5 subtype highly pathogenic avian influenza virus at home and abroad, including agricultural industry standards or national standar...

AI Technical Summary

Problems solved by technology

At present, various nucleic acid detection methods for H5 subtype highly pathogenic avian influenza virus at home and abroad, including agricultural industry standards or national standards, take a long time, such as agricultural industry standards (RT-PCR detection method for avian influenza virus, NY / T 772–2013) takes at least 2 hours, and the national standard (H5 subtype avian influenza virus fluorescent RT-PCR detection method, GB / T 19438.2–2004) needs at least 1.2 hours. Currently, there is no more efficient detection method than these methods method