Preparation method of transferrin and Fe combined Fe supplementing agent
A technology of iron supplement and protein iron, which is applied in the field of blood supplements, can solve the problems that users are difficult to insist on taking for a long time, it is not easy for consumers to accept, and it interferes with drug metabolism, so as to reduce side effects and adverse reactions, and have fewer side effects and adverse reactions , cost reduction effect
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Embodiment 1
[0021] (1) Disperse commercially available magnetic iron oxide nanoparticles (with an average particle size of less than 100 nm) based on ferric oxide in a phosphate buffer with a pH of 8 at a ratio of 0.3 g / mL, and then press Add 2.5% glutaraldehyde to the volume of 2.5%, and stir in the dark for 3 h to cross-link glutaraldehyde and magnetic iron oxide nanoparticles. Wash off the uncross-linked glutaraldehyde molecules with distilled water, ultrasonically treat the nanoparticles for 10 s to disperse the nanoparticles, and then Add ferric ions (FeCl) to the solution 3 , 20mmol / L), stirred and reacted for 5h, and distilled water to wash away excess iron ions to prepare a magnetic nanoparticle carrier;
[0022] (2) dilute the egg white by 8 times, adjust the pH to 8.5, then add the magnetic nanoparticle carrier by 0.5% by weight of the solution, and stir for 10 minutes to make the ovotransferrin in the solution and the ferric iron on the magnetic nanoparticle carrier ionic bond...
Embodiment 2
[0026] (1) is identical with step (1) of embodiment 1;
[0027] (2) Dilute the milk by 5 times, adjust the pH of the solution to 6.8, then add the magnetic nanoparticle carrier by 0.02% by weight of the solution, and stir for 5 minutes to make the transferrin in the solution and the ferric ion on the magnetic nanoparticle carrier combine;
[0028] (3) Use a magnet to adsorb the transferrin-adsorbed magnetic nanoparticle carrier to the bottom of the solution, discard the upper layer solution, wash the lower layer solid with a pH 6.8 buffer, then transfer it to a pH 3.2 buffer, stir for 60 minutes to release the bound transferrin from the magnetic nanoparticle carrier; the magnetic nanoparticle carrier and the solution were separated by a magnet again, and FeCl was added to the solution 3 (20mmol / L) reaction, obtains iron-binding transferrin after drying;
[0029] (4) Take 100 g of iron-binding transferrin, 0.1 g of ferrous fumarate, 1 g of L-ascorbic acid, and 20 g of jujube ...
Embodiment 3
[0031] (1) is identical with step (1) of embodiment 1;
[0032] (2) dilute the egg white by 10 times, adjust the pH of the solution to 9.5, then add the magnetic nanoparticle carrier by 1% by weight of the solution, and stir for 60 minutes to make the transferrin in the solution and the ferric iron on the magnetic nanoparticle carrier ionic bonding;
[0033] (3) Use a magnet to adsorb the transferrin-adsorbed magnetic nanoparticle carrier to the bottom of the solution, discard the upper layer solution, wash the lower layer solid with a pH 9.5 buffer, then transfer it to a pH 4.8 buffer, stir for 5 minutes to release the bound transferrin from the magnetic nanoparticle carrier; the magnetic nanoparticle carrier and the solution were separated by a magnet again, and FeCl was added to the solution 3 (5mmol / L) reaction, obtains iron-binding transferrin after drying;
[0034] (4) Take 100 g of iron-binding transferrin, 0.5 g of ferrous fumarate, 5 g of L-ascorbic acid, and 5 g of...
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