Preparation method of transferrin and Fe combined Fe supplementing agent

A technology of iron supplement and protein iron, which is applied in the field of blood supplements, can solve the problems that users are difficult to insist on taking for a long time, it is not easy for consumers to accept, and it interferes with drug metabolism, so as to reduce side effects and adverse reactions, and have fewer side effects and adverse reactions , cost reduction effect

Active Publication Date: 2016-10-12
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are dozens of types of commonly used iron supplements, but the existing iron supplements have some disadvantages: they have a heavy iron smell, which is not easily accepted by consumers; it is easy to cause gastrointestinal discomfort, and users are very It is difficult to take it consistently for a long time, and the tolerance is poor; some iron supplements will interfere with the metabolism of drugs

Method used

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  • Preparation method of transferrin and Fe combined Fe supplementing agent
  • Preparation method of transferrin and Fe combined Fe supplementing agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] (1) Disperse commercially available magnetic iron oxide nanoparticles (with an average particle size of less than 100 nm) based on ferric oxide in a phosphate buffer with a pH of 8 at a ratio of 0.3 g / mL, and then press Add 2.5% glutaraldehyde to the volume of 2.5%, and stir in the dark for 3 h to cross-link glutaraldehyde and magnetic iron oxide nanoparticles. Wash off the uncross-linked glutaraldehyde molecules with distilled water, ultrasonically treat the nanoparticles for 10 s to disperse the nanoparticles, and then Add ferric ions (FeCl) to the solution 3 , 20mmol / L), stirred and reacted for 5h, and distilled water to wash away excess iron ions to prepare a magnetic nanoparticle carrier;

[0022] (2) dilute the egg white by 8 times, adjust the pH to 8.5, then add the magnetic nanoparticle carrier by 0.5% by weight of the solution, and stir for 10 minutes to make the ovotransferrin in the solution and the ferric iron on the magnetic nanoparticle carrier ionic bond...

Embodiment 2

[0026] (1) is identical with step (1) of embodiment 1;

[0027] (2) Dilute the milk by 5 times, adjust the pH of the solution to 6.8, then add the magnetic nanoparticle carrier by 0.02% by weight of the solution, and stir for 5 minutes to make the transferrin in the solution and the ferric ion on the magnetic nanoparticle carrier combine;

[0028] (3) Use a magnet to adsorb the transferrin-adsorbed magnetic nanoparticle carrier to the bottom of the solution, discard the upper layer solution, wash the lower layer solid with a pH 6.8 buffer, then transfer it to a pH 3.2 buffer, stir for 60 minutes to release the bound transferrin from the magnetic nanoparticle carrier; the magnetic nanoparticle carrier and the solution were separated by a magnet again, and FeCl was added to the solution 3 (20mmol / L) reaction, obtains iron-binding transferrin after drying;

[0029] (4) Take 100 g of iron-binding transferrin, 0.1 g of ferrous fumarate, 1 g of L-ascorbic acid, and 20 g of jujube ...

Embodiment 3

[0031] (1) is identical with step (1) of embodiment 1;

[0032] (2) dilute the egg white by 10 times, adjust the pH of the solution to 9.5, then add the magnetic nanoparticle carrier by 1% by weight of the solution, and stir for 60 minutes to make the transferrin in the solution and the ferric iron on the magnetic nanoparticle carrier ionic bonding;

[0033] (3) Use a magnet to adsorb the transferrin-adsorbed magnetic nanoparticle carrier to the bottom of the solution, discard the upper layer solution, wash the lower layer solid with a pH 9.5 buffer, then transfer it to a pH 4.8 buffer, stir for 5 minutes to release the bound transferrin from the magnetic nanoparticle carrier; the magnetic nanoparticle carrier and the solution were separated by a magnet again, and FeCl was added to the solution 3 (5mmol / L) reaction, obtains iron-binding transferrin after drying;

[0034] (4) Take 100 g of iron-binding transferrin, 0.5 g of ferrous fumarate, 5 g of L-ascorbic acid, and 5 g of...

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Abstract

The invention discloses a preparation method of a transferrin and Fe combined Fe supplementing agent. According to the preparation method, a magnetic nanoparticle carrier is subjected to a reaction with a sample solution, transferrin in the sample solution is enabled to be bound with Fe<3+> on the magnetic nanoparticle carrier; then the magnetic nanoparticle carrier with the transferrin adsorbed is separated from the solution by a magnet, through regulation of the pH value, the bound transferrin is released again from the magnetic nanoparticle carrier to be reacted with FeCl3, and Fe-binding transferrin is obtained after drying; the Fe-binding transferrin is mixed with ferrous fumarate, L-ascorbic acid, a blood supplementing traditional Chinese medicine extract and a pharmaceutically acceptable carrier, and the transferrin and Fe combined Fe supplementing agent is prepared. The preparation method has the advantages that the Fe supplementing effect is good, few side effects and adverse reactions are produced and the like.

Description

technical field [0001] The invention belongs to the field of blood supplements, and in particular relates to a preparation method of a protein iron supplement. Background technique [0002] Iron ion is an essential trace element for the human body. It participates in the synthesis of hemoglobin and is the guarantee for the body to transport and supply oxygen. Iron deficiency is an important factor causing anemia. More than 50% of anemia (ie iron deficiency anemia, IDA) is caused by iron deficiency. Nutritional fortification of iron deficiency people through iron supplements is a preventive and therapeutic method. Effective measures for iron deficiency and iron deficiency anemia. [0003] At present, there are dozens of types of commonly used iron supplements, but the existing iron supplements have some disadvantages: they have a heavy iron smell, which is not easy for consumers to accept; it is easy to cause gastrointestinal discomfort, and users are very uncomfortable. It...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K33/26A61K47/48A61P7/06A61K35/36A61K36/725A61K36/815
CPCA61K33/26A61K35/36A61K36/725A61K36/815A61K2300/00
Inventor 马美湖耿放袁小军任国栋何恩祺付星王一博
Owner HUAZHONG AGRI UNIV
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