A preparation method of functionally graded hydrogel for bone-cartilage repair

A cartilage repair and functional gradient technology, applied in the field of preparation of functionally gradient hydrogels, can solve the problems of limited cartilage layer thickness, long-term activity reduction, subchondral bone damage, etc., to achieve improved mechanical properties, good mechanical properties, and protective activity Effect

Inactive Publication Date: 2018-12-25
SOUTHWEST JIAOTONG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, traditional degradable natural polymer hydrogels have the following disadvantages: the mechanical properties of hydrogels are poor, and it is difficult to adapt to various mechanical environments in joints, which limits their application as osteochondral replacement materials; secondly, the cartilage layer Thickness is limited, cartilage damage will cause subchondral bone damage, and subchondral bone damage can affect the metabolism of the cartilage layer. Simple cartilage replacement materials are not conducive to the repair of cartilage morphology and structural functions; The structure of cartilage is similar, which is helpful for the repair of osteocartilage, but the current research shows that the interface between the upper and lower layers of the double-layer hydrogel is poorly bonded, and gradient function repair cannot be achieved. At the same time, the upper layer of hydrogel is easy to cause short-term release of adsorption factors through swelling and soaking High volume, long-term activity reduction and other problems

Method used

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  • A preparation method of functionally graded hydrogel for bone-cartilage repair

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Embodiment 1

[0040] A method for preparing a functionally graded hydrogel for bone-cartilage repair, the specific steps are as follows:

[0041] Step A: Introduce double bonds on the gelatin by methacrylic anhydride to obtain double bonded gelatin, the specific steps are as follows:

[0042] A1: Weigh gelatin and dissolve it in phosphate buffer saline, and prepare a solution with a mass fraction of 5% under water bath conditions;

[0043] A2: Add 0.1 times the mass of methacrylic anhydride dropwise to the gelatin solution at a rate of 1ml / min, and then stir for 3 hours;

[0044] A3: adding phosphate buffer solution to the reaction solution of gelatin and methacrylic anhydride for dilution to terminate the reaction;

[0045] A4: The solution diluted in step A3 was placed in a dialysis bag with a molecular weight cut-off of 12 kDa, dialyzed with deionized water and freeze-dried to obtain double-bonded gelatin.

[0046] The double-bonded gelatin is prepared as a monomer into two parts of aq...

Embodiment 2

[0052] A method for preparing a functionally graded hydrogel for bone-cartilage repair, the specific steps are as follows:

[0053] Step A: Introduce double bonds on the gelatin by methacrylic anhydride to obtain double bonded gelatin, the specific steps are as follows:

[0054] A1: Weigh gelatin and dissolve it in phosphate buffer saline, and prepare a solution with a mass fraction of 5% under water bath conditions;

[0055] A2: Add 0.1 times the mass of methacrylic anhydride dropwise to the gelatin solution at a rate of 1ml / min, and then stir for 3 hours;

[0056] A3: adding phosphate buffer solution to the reaction solution of gelatin and methacrylic anhydride for dilution to terminate the reaction;

[0057] A4: The solution diluted in step A3 was placed in a dialysis bag with a molecular weight cut-off of 14 kDa, dialyzed with deionized water and freeze-dried to obtain double-bonded gelatin.

[0058] The double-bonded gelatin is formulated into two parts of aqueous solut...

Embodiment 3

[0064] A method for preparing a functionally graded hydrogel for bone-cartilage repair, the specific steps are as follows:

[0065] Step A: Introduce double bonds on the gelatin by methacrylic anhydride to obtain double bonded gelatin, the specific steps are as follows:

[0066] A1: Weigh gelatin and dissolve it in phosphate buffer saline, and prepare a solution with a mass fraction of 10% under water bath conditions;

[0067] A2: Add 0.1 times the mass of methacrylic anhydride dropwise to the gelatin solution at a rate of 0.5ml / min, and then stir for 3 hours;

[0068] A3: adding phosphate buffer solution to the reaction solution of gelatin and methacrylic anhydride for dilution to terminate the reaction;

[0069] A4: The solution diluted in step A3 was placed in a dialysis bag with a molecular weight cut-off of 12 kDa, dialyzed with deionized water and freeze-dried to obtain double-bonded gelatin.

[0070] Prepare the double-bonded gelatin as a monomer into two parts of aqu...

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Abstract

The invention discloses a preparation method of a functionally graded hydrogel for bone-cartilage repair, and belongs to the technical field of biomaterials. In the present invention, two double-bond biomacromolecule pre-polymerization liquids with the same mass fraction are first prepared, and then the pre-polymerization liquids are respectively prepared into an upper cartilage repair pre-polymerization liquid containing repair factor nanoparticles and a lower subchondral liquid containing calcium phosphorus nanoparticles. The bone repair pre-polymerization liquid is based on the principle of suspension and photopolymerization, and forms a functionally gradient hydrogel whose function changes with changes in composition and structure through the sedimentation and diffusion of nanoparticles. The biomimetic hydrogel prepared by the invention has good biodegradability and biocompatibility, high efficiency of loaded growth factors, good slow-release performance, can induce the repair of cartilage and subchondral bone, and realize the two-way of bone-cartilage Regeneration, to meet the repair of articular cartilage damage.

Description

technical field [0001] The invention belongs to the technical field of biomaterials, and in particular relates to a preparation method of a functionally gradient hydrogel for bone-cartilage repair. Background technique [0002] Articular cartilage injury is one of the most challenging clinical problems. At present, the clinical treatment methods for articular cartilage repair mainly include microfracture, autologous cartilage transplantation and allogeneic cartilage transplantation. The ability of cartilage regeneration and self-repair is extremely limited, and the existing artificial cartilage prosthesis has problems such as poor biological activity and poor integration with subchondral bone. [0003] Hydrogel is a cross-linked three-dimensional network polymer with hydrophilic groups that can swell in water but is insoluble in water. It is an important functional polymer material and is a research hotspot in current materials science. one. Hydrogels contain hydrophilic g...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/18A61L27/52A61L27/54A61L27/12A61L27/16
CPCA61L27/12A61L27/16A61L27/52A61L27/54A61L2300/112A61L2300/414A61L2430/02A61L2430/06C08L51/02C08L51/00
Inventor 鲁雄王志雄韩璐胥杰龙
Owner SOUTHWEST JIAOTONG UNIV
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