Method for nondestructively determining content of phytic acid in whole cottonseed based on near infrared spectroscopy

Abstract

The invention discloses a method for non-destructive determination of phytic acid content in whole cottonseeds based on near-infrared spectroscopy. Prepare whole-grain cottonseed samples of different varieties planted in different regions for many years, and collect full-spectrum data of whole-grain cottonseed samples; use a variety of preprocessing methods to preprocess the full-spectrum data, and use high-efficiency ion chromatography to accurately determine phytic acid in samples Content, using the full cross-validation method combined with the multivariate calibration regression method to establish multiple calibration models in the full spectrum range, determine the optimal near-infrared spectrum calibration model, and use the calibration model to detect the whole cottonseed sample to be tested to obtain its phytic acid content. The invention collects the spectrogram of the whole cottonseed, has fast measurement speed, high accuracy, and does not need chemical reagents, is a method for non-destructively analyzing the phytic acid content in the whole cottonseed, and is useful for cultivating low-phytic acid cotton varieties and promoting the processing of cotton by-products Utilization is important.

Description

technical field [0001] The invention discloses a method for measuring the content of agricultural by-products. It specifically relates to a non-destructive determination method of phytic acid content in whole cotton seeds based on near-infrared spectroscopy, and uses near-infrared spectroscopy and chemometric methods to conduct non-destructive analysis of phytic acid content in whole cotton seeds. Background technique [0002] Cottonseed is the main by-product of cotton. The annual output of cottonseed is more than 10 million tons nationwide, and the annual output of cottonseed cake is more than 6 million tons. It is widely distributed and has the largest resource volume in the world. Cottonseed is rich in protein (27.83-45.60%) and fat (28.24-44.05%). After shelling, cottonseed oil and cottonseed cake are obtained by pressing. The cottonseed oil is edible, and the cottonseed cake can be used as feed for poultry and livestock. The protein content in cottonseed cake is secon...

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Problems solved by technology

[0004] The content of phytic acid in cottonseed is an important indicator that affects the comprehensive utilization of cottonseed. The determination of phytic acid content by conventional chemical methods takes a long time to analyze, the cost of detection is high, and a large amount of toxic chemical reagents are consumed, which pollutes the environment and has low safety. The comprehensive evaluation of substances increases the difficulty

More importantly, the analysis of phytic acid content in cottonseed by traditional chemical methods requires shelling and grinding of cottonseed, which will not only consume the test sample, make the test sample unable to be returned to use, and consume a lot of time, but also affect the phytic acid content The accuracy of determination hinders its comprehensive utilization in cottonseed

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