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Detection method for residual quantity of phoxim in animal tissue

A detection method and animal tissue technology, which can be used in measurement devices, instruments, scientific instruments, etc., can solve the problems of complex operation process, long detection time, large sampling volume, etc., and achieve accurate detection results, short detection time, and easy operation. Effect

Inactive Publication Date: 2016-10-12
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method has large sampling volume, complex operation process, and long detection time, making it difficult to quickly obtain detection results.
Moreover, there is no report on the detection of phoxim residues in animals such as SD rats and pigs
[0005] Since SD rats and other experimental animals are sensitive to chemical pesticides, their symptoms after ingesting different levels of pesticide poisoning are similar, so the diagnosis cannot be made based on the symptoms

Method used

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  • Detection method for residual quantity of phoxim in animal tissue
  • Detection method for residual quantity of phoxim in animal tissue
  • Detection method for residual quantity of phoxim in animal tissue

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Determination method of phoxim residues in animal liver tissue

[0028] (l) Materials and equipment:

[0029] The animal species is SD rats. Pesticides adopt phoxim standard substance (chromatographically pure, Sigma company product, number: 14816-18-3), methanol (chromatographically pure, Sigma company product, number: 67-56-1), dichloromethane (analytical pure, Tianjin Fuyu products).

[0030] The high-performance liquid chromatograph is a product of WATERS Company in the United States (WATERS 6000), including a WATERS2487 ultraviolet detector; the chromatographic column ODSC18 is a product of PG instruments Ltd.

[0031] (2) Administration and sampling:

[0032] The conventional feeding method was adopted, and the poisoning was carried out by oral gavage at 8:00 a.m. every day. Soybean oil was used as the solvent, the poisoning dose was 0.5 mL, and the poisoning concentration was 180 mg / kg·BW. Weigh once a day to adjust the feeding amount. Once a day, 7 days a w...

Embodiment 2

[0054] Determination of phoxim residues in jejunum tissue after removal of intestinal contents

[0055] The conventional feeding method was adopted, and the poisoning was carried out by oral gavage at 8:00 a.m. every day. Soybean oil was used as the solvent, the poisoning dose was 0.5 mL, and the poisoning concentration was 180 mg / kg·BW. Weigh once a day to adjust the feeding amount. Once a day, 7 days a week, for a total of 30 days. After feeding, the rats were put back into the cages, with free access to water and food. On the 30th day of the rat experiment, blood was collected and slaughtered for laboratory analysis. The jejunal tissue samples were washed with normal saline and placed in sample bags, labeled and stored in a -20°C refrigerator.

[0056] Take 0.10 g of SD rat jejunum tissue sample, grind it thoroughly in liquid nitrogen, transfer it to a mortar containing 1.5-2.0 g of diatomaceous earth / magnesium trisilicate and continue grinding. Fold the two pieces of r...

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Abstract

The invention discloses a detection method for the residual quantity of phoxim in animal tissue. The detection method comprises the following steps that 1, a sample to be detected is firstly eluted with dichloromethane and then blown dry through 37-DEG C nitrogen to obtain an extract; 2, the extract is dissolved into methanol, and then filtering is conducted with a 0.45-micrometer organic filter membrane to obtain an extract solution; 3, detection is conducted by taking a methanol-water solution as a mobile phase through high performance liquid chromatography to determine the residual quantity of phoxim in the animal tissue. Compared with a method for detecting the residual quantity of phoxim in vegetables and fruits in the prior art, the detection method has the advantages of being little in sampling amount, short in detection time, easy and convenient to operate and the like by adopting the high performance liquid chromatography; the detection result is accurate, the phoxim detection limit is 0.01 mg / L, and the general chemical pesticide residual quantifying requirements can be met.

Description

technical field [0001] The invention relates to a method for detecting pesticide residues, in particular to a method for detecting phoxim residues in animal tissues. Background technique [0002] my country is a large agricultural country, and chemical control has played an important role in the prevention and control of major diseases and insect pests of food crops. After the application of pesticides, most of them are metabolized, degraded and migrated in the natural environment, but improper use of pesticides can not only cause environmental pollution, but also cause pesticide residues on crops, and enter livestock and poultry through food chain accumulation and transfer, resulting in Pesticide residues in poultry products endanger human health and cause a series of continuous adverse consequences. Therefore, pesticide residues not only affect the quality and safety level of my country's livestock and poultry products and export trade, but more importantly, bring direct ...

Claims

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Application Information

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IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/027
Inventor 单安山张婧宋文涛孙岳丞
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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