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Method for measuring ethephon residual quantity in fruits and vegetables

A technology of ethephon and residues, applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of unsuitable storage, mucosal acid corrosion, irritating odor, etc., to avoid flammable and explosive, simple and safe operation, and detection. Accurate results

Pending Publication Date: 2019-11-19
JILIN PONY TESTING TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The pretreatment method in the national standard is complicated, and requires a lot of pretreatment reagents and consumables, and the derivative reagent diazomethane has the characteristics of pungent odor and explosive, so it is not suitable for storage, and the experiment process has great safety hazards
[0006] Due to the specificity of the period and method of use of ethephon, and its irritating effect on the skin and eyes, it has an acid erosion effect on the mucous membrane, and may cause pollution to the environment and water bodies

Method used

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  • Method for measuring ethephon residual quantity in fruits and vegetables
  • Method for measuring ethephon residual quantity in fruits and vegetables
  • Method for measuring ethephon residual quantity in fruits and vegetables

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] The condition selection of embodiment 1 headspace gas phase method to measure ethephon

[0052] Preparation of standard working solution:

[0053]Weigh 25.0 mg (accurate to 0.01 mg) of ethephon standard substance (CAS number: 16672-87-0) with a purity ≥ 98.5%, and place it in a 25 mL brown volumetric flask filled with about 2-3 mL of 0.081 mol / L hydrochloric acid solution , mix and dissolve, make up the volume with 0.081mol / L hydrochloric acid solution, configure to a concentration of 1000μg / mL, store in cold storage, and have a validity period of 6 months. Then gradually use 0.081mol / L hydrochloric acid solution to prepare standard working solutions of 0.2, 1.0, 2.0, 4.0 and 10.0μg / mL.

[0054] Detection method: put 2.0g (accurate to 0.001g) of the sample to be tested into a headspace sample bottle, add distilled water and potassium hydroxide solution, and inject the sample into the headspace under constant temperature conditions, and use a hydrogen flame detector to ...

Embodiment 2

[0067] Embodiment 2 linear range, detection limit and limit of quantitation evaluation

[0068] Linear range: using the standard working solutions of different concentrations prepared in the examples, take the peak area as the ordinate, and the mass concentration of the standard working solution as the abscissa to draw a standard curve. The results show that within the range of 0.2 to 10.0 μg / mL, the mass of ethephon The linear relationship between the concentration and the corresponding peak area is good, and the correlation coefficient is greater than 0.999.

[0069] Limit of detection and limit of quantitation: Add standard working solution to the blank sample to prepare ethephon standard solutions with final concentrations of 0mg / kg, 0.025mg / kg, 0.05mg / kg, 0.1mg / kg, and 0.2mg / kg. According to the testing method of embodiment 1, test its signal-to-noise ratio, determine detection line and limit of quantification respectively, obtain the detection limit (LOD) of target objec...

Embodiment 3

[0070] Example 3 sample detection recovery and precision

[0071] 1. Sample pretreatment: Take negative fruit and vegetable samples such as kiwi, mango, banana, cucumber, zucchini and tomato to homogenate well, and add ethephon at three concentration levels of 0.0100, 0.0200 and 0.0500mg / kg to different matrix samples respectively, and weigh them respectively Take 2.0 g of spiked samples of different matrices in a headspace vial, add 3 mL of distilled water and 5 mL of potassium hydroxide solution, the distilled water is secondary distilled water, and the concentration of potassium hydroxide solution is 1 g / mL.

[0072] 2. Sample detection: draw 1mL of each concentration standard working solution prepared in the example into a 20mL headspace bottle, add 3mL of distilled water and 5mL of 1g / mL potassium hydroxide solution, cover and seal it, and put it on the machine under the same conditions as the sample to be tested , under the condition of 80 ℃ constant temperature headspac...

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Abstract

The invention discloses a method for measuring ethephon residual quantity in fruits and vegetables, and the method comprises the following steps: S1, soaking a homogenized sample to be detected in a headspace bottle by using distilled water and a potassium hydroxide solution; S2, placing the headspace bottle in an automatic incubator for constant temperature treatment, so that the decomposed ethylene is balanced in water and air; and S3, detecting by using a gas chromatograph with a hydrogen flame ionization detector by taking nitrogen as carrier gas, and determining the residual quantity of ethephon in the fruits and vegetables. The method for measuring ethephon residual quantity in fruits and vegetables has the advantages of less sampling amount, short detection time, simple and safe operation and the like, has mild reaction, and avoids the use of flammable and explosive reagents with toxicity and irritation; and the method for measuring ethephon residual quantity in fruits and vegetables has accurate detection result, the ethephon detection limit is 0.05 mg / kg, and the requirement of quantitative detection of ethephon drug residue of fruits and vegetables can be met.

Description

technical field [0001] The invention relates to the technical field of pesticide residue detection, in particular to a method for measuring ethephon residues in fruits and vegetables. Background technique [0002] As a developing country in China, agriculture has always been the basic pillar of our survival, and pesticides play an important role as a solid guarantee for crop quality and yield. As my country began to use pesticides in the 1930s and 1940s, the problem of residues after pesticide application also followed. Although today's pesticides are basically low-toxic, easy to decompose, and low-residue formulations and ingredients, pesticides will still pollute the natural environment after use, especially some pesticides that have functions such as freshness preservation and ripening will directly contact crops In fact, due to improper use, it will endanger the health of humans and livestock, causing a series of continuous adverse consequences. [0003] According to t...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 宋薇李闯李佳王占峰冯辉甄岩
Owner JILIN PONY TESTING TECH CO LTD
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