Gene for improving plant disease resistance and application thereof

A disease resistance and plant technology, applied in the field of genetic engineering, can solve the problems of large differences in promoter regions and different induced expression patterns

Inactive Publication Date: 2016-10-26
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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Problems solved by technology

Sequence analysis of the gene revealed that although there was no difference in the coding region of the gene in the resistant materials, their promoter reg

Method used

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  • Gene for improving plant disease resistance and application thereof
  • Gene for improving plant disease resistance and application thereof
  • Gene for improving plant disease resistance and application thereof

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Embodiment Construction

[0059]The methods used in the following embodiments are conventional methods unless otherwise specified, and the primer sequences used are all synthesized by Shanghai Handsome Biotechnology Co., Ltd., and the stated percentages are all mass percentages. The tomato materials used in this experiment were the TYLCV-resistant CLN2777A and the susceptible variety TMXA48-4-0 (Chen T, Lv Y, Zhao T, Li N, Yang Y, Yu W, HeX, Liu T, and Zhang B*.Comparative Transcriptome Profiling of a Resistant vs. Susceptible Tomato (Solanum lycopersicum) Cultivar in Response to Infection by Tomato Yellow Leaf Curl Virus. PLoS One, 2013, 8(11):e80816). The tobacco is Nicotiana benthamiana. Both tomato and tobacco were grown in a plant growth chamber. The light intensity is 130 μmolphotons m –2 the s –1 , with a humidity of 65%.

[0060] 1. Acquisition of LeAct1 and bioinformatics analysis

[0061] In our laboratory, we analyzed transcriptome differences before and after inoculation of anti-suscep...

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Abstract

The invention discloses a gene for improving the plant disease resistance and application thereof. The gene LeAct1 codes an agmatine transferase, which has a DNA (Deoxyribonucleic Acid) sequence shown as SEQ ID NO.1 in a sequence table, or has a DNA sequence capable of being hybridized with SEQ ID NO.1 under the strictly strict condition, or the gene LeAct1 codes a DNA sequence with the same amino acid sequence as the SEQ ID NO.1 in the sequence table. The gene is transformed and imported into a receiver plant genome; the agmatine transferase is expressed; and the disease resistance capability of a receiver plant can be improved. When the gene is applied to the agricultural biological technology breeding for improving the crop disease-resistant properties, the breeding period can be greatly shortened; and the breeding efficiency is improved.

Description

technical field [0001] The invention belongs to the field of genetic engineering, in particular to a gene for improving plant disease resistance and its application. Background technique [0002] Tomato yellow leaf curl disease (TYLCD) is a devastating disease that is extremely difficult to control in tomato production. The disease was first discovered in the Jordan River area of ​​Israel, and then broke out in large areas around the world. The harm is becoming more and more serious in China (Antignus&Cohen, 1994; Boulton, 2003; Guoyanmei et al., 2009). The disease is caused by geminiviruses, especially Tomato yellow leaf curl virus (TYLCV) is the most harmful in China (Li Jiawei et al., 2016). There is a lack of TYLCV resistance genes in tomato cultivation germplasm resources, and the resistance genes Ty-1-Ty-5 identified so far are all derived from wild relatives. Among them, Ty-2 has a significantly stronger inhibitory effect on virus replication than Ty-1 / Ty-3, and the...

Claims

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Application Information

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IPC IPC(8): C12N15/54C12N15/82A01H5/00
CPCC12N9/1029C12N15/8282C12N15/8283C12Y203/01064
Inventor 杨郁文胡节立张保龙王金彦
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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