Freezing and unfreezing methods of boar semen

A freezing method and a technique of pig semen, which are applied in the field of freezing and thawing of pig semen to achieve the effects of improving utilization rate, reducing cost, and making great genetic progress

Inactive Publication Date: 2016-11-09
SHANDONG XINJI ANIMAL HUSBANDRY
View PDF3 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in my country's pig production, the work carried out on the research and application of frozen semen is still lacking. Therefore, in-depth research and application of this technology is not only of great significance for further

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0018] Below in conjunction with specific embodiment, the present invention is described in further detail, but implementation method of the present invention is not limited to the scope that embodiment shows, and this embodiment only limits to illustrate the present invention, and is not intended to limit the scope of the present invention.

[0019] A method for freezing and thawing porcine semen, comprising a method for freezing porcine semen, a method for thawing porcine semen and detection of porcine semen, wherein the method for freezing porcine semen comprises the following steps:

[0020] Step 1: Collect excellent breeding boar semen and measure its density, then dilute the semen with a diluent, the temperature of the diluted semen is 37.5-40°C, and dilute until the ratio of the final number of live sperm to the boar semen is 0.50-0.51:1.

[0021] Step 2: The semen diluted in Step 1 is allowed to stand at room temperature of 17.5-19.5° C. for 0.5-1 hour.

[0022] Step ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses freezing and unfreezing methods of boar semen. The methods comprise a boar semen freezing method, a boar semen unfreezing method and a boar semen detecting method. The boar semen freezing method comprises the following steps: collecting breeding boar semen, determining the density of the breeding boar semen, and diluting the breeding boar semen; allowing the diluted semen to stand, and carrying out cooling balancing; centrifuging the cooled semen at 14-15 DEG C; sequentially adding a dilution solution I and a dilution solution II, and packaging the obtained semen; and freezing the packaged semen, and carrying out freeze preservation by using liquid nitrogen. The unfreezing method comprises the following steps: rapidly taking the frozen boar semen, heating the frozen boar semen to 5 DEG C from -140 DEG C at a rate of 50 DEG C/min, and heating the heated boar semen to 5 DEG C from -5 DEG C according to a rate of 3 DEG C/min in order to carrying out unfreezing. The invention provides a technology for promotion of the application of the frozen boar semen to production.

Description

technical field [0001] The invention belongs to the technical field of cryopreservation of pig semen, in particular to a freezing and thawing method of pig semen. Background technique [0002] The artificial insemination (AI) technology of pigs is the use of artificial methods to collect semen from boars, perform quality inspection, dilution and storage, etc., and then use equipment to transport the semen to the reproductive organs of estrus sows to impregnate them . Artificial insemination can improve the breeding performance of boars. Compared with the original cross, the number of sows mated by an excellent boar can be increased by 10 to 20 times per year during artificial insemination. Due to the increase in the utilization rate of fine-bred boars, the improvement of breeds has been accelerated, which can bring huge benefits to pig production and pig breeding; reduce the number of boars raised, and save feeding and management costs; it can overcome the difficulties of n...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A01N1/02
CPCA01N1/0284
Inventor 吴衍岭陈宪彬刘晶乔富兴彭兴泉袁震侯磊
Owner SHANDONG XINJI ANIMAL HUSBANDRY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap