Freezing and unfreezing methods of boar semen

Abstract

The invention discloses freezing and unfreezing methods of boar semen. The methods comprise a boar semen freezing method, a boar semen unfreezing method and a boar semen detecting method. The boar semen freezing method comprises the following steps: collecting breeding boar semen, determining the density of the breeding boar semen, and diluting the breeding boar semen; allowing the diluted semen to stand, and carrying out cooling balancing; centrifuging the cooled semen at 14-15 DEG C; sequentially adding a dilution solution I and a dilution solution II, and packaging the obtained semen; and freezing the packaged semen, and carrying out freeze preservation by using liquid nitrogen. The unfreezing method comprises the following steps: rapidly taking the frozen boar semen, heating the frozen boar semen to 5 DEG C from -140 DEG C at a rate of 50 DEG C / min, and heating the heated boar semen to 5 DEG C from -5 DEG C according to a rate of 3 DEG C / min in order to carrying out unfreezing. The invention provides a technology for promotion of the application of the frozen boar semen to production.

Description

technical field [0001] The invention belongs to the technical field of cryopreservation of pig semen, in particular to a freezing and thawing method of pig semen. Background technique [0002] The artificial insemination (AI) technology of pigs is the use of artificial methods to collect semen from boars, perform quality inspection, dilution and storage, etc., and then use equipment to transport the semen to the reproductive organs of estrus sows to impregnate them . Artificial insemination can improve the breeding performance of boars. Compared with the original cross, the number of sows mated by an excellent boar can be increased by 10 to 20 times per year during artificial insemination. Due to the increase in the utilization rate of fine-bred boars, the improvement of breeds has been accelerated, which can bring huge benefits to pig production and pig breeding; reduce the number of boars raised, and save feeding and management costs; it can overcome the difficulties of n...

AI Technical Summary

Problems solved by technology

However, in my country's pig production, the work carried out on the research and application of frozen semen is still lacking. Therefore, in-depth research and application of this technology is not only of great significance for further exerting the advantages of artificial insemination technology, but also It is also of great strategic significance for the genetic improvement of breeding pigs and the preservation of some endangered and unique local pig breed resources.