Medicinal composition of carbocisteine and treatment effects of medicinal composition for leukemia

A technology of carbocisteine ​​and a composition is applied in the directions of drug combinations, medical raw materials derived from ferns/filamentous plants, steroids, etc., and can solve the problems such as no reports on the relevance of carbocisteine, and achieves Prominent substantive features, significant treatment effects, and significantly improved effects

Inactive Publication Date: 2016-11-09
赵吉永
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] So far, there has been no report on the correlation between carbocisteine ​​and its pharmaceutical composition and leukemia

Method used

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  • Medicinal composition of carbocisteine and treatment effects of medicinal composition for leukemia
  • Medicinal composition of carbocisteine and treatment effects of medicinal composition for leukemia
  • Medicinal composition of carbocisteine and treatment effects of medicinal composition for leukemia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1: Compound (I) Separation Preparation and Structure Confirmation

[0018] Separation method: (a) crush the dry leaves (2kg) of Shiwei, extract with 75% ethanol under heat reflux (20L×3 times), combine the extracts, concentrate until no alcohol smell (4L), and then use petroleum ether (4L ×3 times), ethyl acetate (4L×3 times) and water-saturated n-butanol (4L×3 times) were extracted to obtain petroleum ether extract, ethyl acetate extract and n-butanol extract respectively; (b) In the step (a), the n-butanol extract is removed with D101 type macroporous resin, first eluted with 15% ethanol for 6 column volumes, then with 70% ethanol for 10 column volumes, collected 70% eluate, and reduced Concentrate under pressure to get 70% ethanol elution concentrate; (c) in step (b), 70% ethanol elution concentrate is separated with normal phase silica gel, and the volume ratio is 80:1 (8 column volumes), 30:1 successively (8 column volumes), 15:1 (8 column volumes) and 5:1...

Embodiment 2

[0021] Embodiment 2: the effect of treating leukemia

[0022] 1. Materials and Instruments

[0023] K562 cells were provided by Shanghai Institute of Biological Cells, Chinese Academy of Sciences. Compound (I) is self-made according to the method of Example 1, and the HPLC normalized purity is greater than 98%. RPMI-1640 dry powder medium was purchased from Gibco. Calf serum was purchased from Hangzhou Sijiqing Institute of Bioengineering Materials. Penicillin and streptomycin were purchased from Huabei Pharmaceutical Factory. Glutamine, MTT, DMSO were purchased from AMRESCO. Annexinv-FITC cell apoptosis detection kit was purchased from Nanjing KGI Biotechnology Development Co., Ltd. MOPS, Rnasin, agarose, olfactory ethidium, bromophenol blue Huamei Bioengineering Company Huamei Bioengineering Company.

[0024] CO 2 Incubator (Thermo Company, USA), ultra-clean bench (Suzhou Purification Equipment Factory), magnetic stirrer (Shanghai Nanhui Telecommunication Equipment Fa...

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Abstract

The invention discloses a medicinal composition of carbocisteine and treatment effects of the medicinal composition for leukemia. The medicinal composition of carbocisteine provided by the invention contains carbocisteine and a natural product compound (I) which is obtained by separating the dried folium pyrrosiae and has the novel structure, when carbocisteine and the natural product act independently, certain treatment effect can be achieved on leukemia, when carbocisteine and the natural product are mixed for use, the treatment effect for leukemia is more obvious, the medicinal composition can be developed into the medicine for treating leukemia, and compared with the prior art, the medicinal composition has outstanding substantive features and obvious progress.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to a new application of carbocisteine, in particular to a pharmaceutical composition of carbocisteine ​​and its therapeutic effect on leukemia. Background technique [0002] Carbocisteine ​​is S-(carboxymethyl) cysteine, which is prepared by reacting cysteine ​​and chloroacetic acid under certain conditions. Carbocisteine ​​is a phlegm-resolving and cough-relieving drug, which mainly affects the secretion of bronchial glands at the cellular level, increases the secretion of low-viscosity salivary protein, reduces the production of high-viscosity fucoidin, and reduces the consistency of sputum, making it easier to cough up , clinically used for chronic bronchitis, bronchial asthma and other diseases caused by thick sputum, difficulty in expectoration, and phlegm obstructing the airway. [0003] So far, there has been no report on the correlation between carbocisteine ​​and its pharmaceutical...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/56A61K31/198A61P35/02
CPCA61K31/198A61K31/56A61K36/11A61K2300/00A61P35/02C07J53/00
Inventor 不公告发明人
Owner 赵吉永
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