Athletic gene detection and evaluation method and system based on qPCR typing technology

It is a technology of motion gene and evaluation system, which is applied to the determination/inspection of microorganisms, biochemical equipment and methods, etc. It can solve the problems of different test results standards, difficulty in uniform evaluation of test results, and poor versatility.

Inactive Publication Date: 2016-11-09
XIAMEN MEIGENE BIOLOGICAL TECH CO LTD
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  • Abstract
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  • Application Information

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Problems solved by technology

Chinese Patent Publication Nos. CN 101851674A, CN 104313147A, CN 102864223A, and CN 104517023A are all combined detection of some sports genes, and the detection results have different standards. Different detection results are difficult to judge uniformly, and the versatility is poor

Method used

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  • Athletic gene detection and evaluation method and system based on qPCR typing technology
  • Athletic gene detection and evaluation method and system based on qPCR typing technology
  • Athletic gene detection and evaluation method and system based on qPCR typing technology

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Experimental program
Comparison scheme
Effect test

Embodiment approach

[0037] See Figure 1~2 As a preferred embodiment of the present invention, the exercise gene detection and evaluation method and system based on qPCR typing technology of this embodiment include the following components:

[0038] a. Personal information database, which includes personal information such as personal name, gender, age, height, weight, BMI and sample number;

[0039] b. Exercise gene detection result database: gene detection based on qPCR typing technology, including 8 qualities of endurance quality, explosive strength quality, soft tissue injury risk, exercise fatigue recovery, exercise enthusiasm, obesity risk, carbohydrate and lipid sensitivity 52 polymorphic loci of 44 related genes, the genotype of each subject is obtained, and the specific loci are shown in the following table:

[0040]

[0041]

[0042] Endurance quality: Endurance refers to the body's ability to maintain a specific intensity load or movement quality within a certain period of time. It is extreme...

Embodiment 2

[0081] Subject Li, the sample collected was a buccal swab, and the genotype involved in Example 1 was sequenced by fluorescent quantitative PCR. The specific sequencing results are as follows:

[0082] (1) Endurance quality

[0083] Li's endurance quality test result is: 46.2 points (the average score of this group is 45.0), ability level: C, C-level people account for 33.7% of the total population. The genotype test results in terms of endurance quality are as follows:

[0084]

[0085]

[0086] (2) Explosive power quality

[0087] Li's explosive power test result is: 63.6 points (the average score of this population is 44.8), ability level: A and C populations account for 1.2% of the total population. In terms of explosive power, the genotype test results are as follows:

[0088] Gene name

genotype

Site effect

Genotype description

AGT

CC

↑↑

Good for explosive strength. Angiotensin II has the highest activity and promotes skeletal muscle growth

AMPD1

CC

↑↑

Good for ...

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Abstract

The invention provides an athletic gene detection and evaluation method based on a qPCR typing technology. The detection method comprises the steps that fluorescent PCR detection is conducted on athletic gene polymorphic sites of detected persons, different contribution degrees are given to the different polymorphic sites, individualized evaluation results are obtained according to statistic analysis on gene detection results and the different contribution degrees, and the evaluation results are summarized to establish an athlete talent gene database. According to the method, the athletic ability and related risks of the detected persons can be comprehensively evaluated, an evaluation foundation is precise, the evaluation efficiency is high, and the evaluation results are stable and accurate; during the athlete recruiting period, the method can assist in an existing talent selecting means for athlete selection; during the training period, individualized training guiding and managing can be conducted on athletes; the defect of finding a single solution for all problems in a traditional method is overcome, and more advantages in athlete selecting and training and the individual body building effect are achieved.

Description

Technical field [0001] The invention relates to the field of biological detection, in particular to a gene detection and evaluation system, in particular to a sports gene detection and evaluation method and system based on qPCR typing technology. Background technique [0002] With the continuous development of scientific research, after the completion of the Human Genome Project, the post-genome project is in full swing, and the application of genetic engineering-led biotechnology in the field of sports problems has received extensive attention. Sports genes are related genes that determine human athletic ability. 70% of athletic ability is affected by genetic factors. Genetic engineering is a general trend for the application of genetic engineering to athlete selection. It can reveal the reasons why athletes are excellent at the molecular level. This technology must be fundamental Cause a revolution in the theory and method of sports selection. At present, preliminary progress ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/68C12Q1/6888C12Q1/6858C12Q2600/156C12Q2537/165
Inventor 吴少鸿姜萍萍任飞李欣詹延延
Owner XIAMEN MEIGENE BIOLOGICAL TECH CO LTD
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