Rapid salicylate spectrophotometric assay method for ammonia nitrogen in water

A technique of ammonia nitrogen salicylate and spectrophotometry, which is applied in the measurement of color/spectral characteristics, analysis by making materials undergo chemical reactions, and material analysis by observing the influence of chemical indicators, etc., can solve the toxicity of Nessler's reagent , cumbersome configuration of reagents, small environmental pollution, etc., to reduce the interference of flocs, speed up the color reaction rate, good recovery rate and precision

Inactive Publication Date: 2016-11-09
淮安自来水有限公司
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AI Technical Summary

Problems solved by technology

For example, when using Nessler's reagent method to detect ammonia nitrogen content in raw water, although it is simple and easy to implement, Nessler's reagent has relatively high toxicity and is likely to cause pollution to the environment. Metal ions and suspended solids such as calcium, magnesium and iron in water will interfere with the determination, so corresponding pretreatment is required
The pretreatment of ammonia nitrogen determination in surface water mostly adopts the flocculation sedimentation-filter paper filtration method. Since the filter paper often contains a small amount of ammonium salt, it needs to be washed and removed repeatedly with ammonia-free water, which increases the complexity of the operation and is difficult to control the parallelism of the experimental results; in addition, It is also possible to directly take the supernatant after flocculation and sedimentation, but sometimes the flocs are not completely settled, which increases the operating time and the repeatability of the measurement results is poor.
[0003] At present, in addition to the Nessler reagent method, the method for detecting ammonia nitrogen in drinking water in GB / T5750.5-2006 in the "Drinking Water Hygienic Standard" also includes the salicylate spectrophotometric method, which is less polluting to the environment. However, the configuration of reagents is cumbersome, and the color development time is long, and it takes 90 minutes at room temperature, which is not conducive to the rapid tracking and detection of this indicator

Method used

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  • Rapid salicylate spectrophotometric assay method for ammonia nitrogen in water
  • Rapid salicylate spectrophotometric assay method for ammonia nitrogen in water
  • Rapid salicylate spectrophotometric assay method for ammonia nitrogen in water

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1: draw the standard curve of ammonia nitrogen

[0029] Use seven 100mL volumetric flasks to configure ammonia nitrogen standard solutions with concentrations of 0 mg / L, 0.025 mg / L, 0.050 mg / L, 0.200 mg / L, 0.500 mg / L, 0.750 mg / L, and 1.000 mg / L respectively; Take 5.00mL standard solutions of different concentrations from each of the above seven 100mL volumetric flasks, and place them in seven 10mL standard colorimetric tubes; add 1.00mL salicylic acid-citrate solution to the standard colorimetric tubes, mix well, Immediately add 0.40mL of chlorine-containing buffer solution and mix thoroughly; put the above-mentioned standard tube in a constant temperature water bath at 30±2°C and heat it for 40 minutes; The absorbance value is shown in Table 1; with the absorbance as the abscissa and the concentration as the ordinate, draw the standard curve of ammonia nitrogen, such as figure 1 shown.

[0030] Table 1 Concentration absorbance table of standard series

[...

Embodiment 2

[0032] Embodiment 2: the precision and accuracy determination of low-concentration and medium-concentration ammonia nitrogen standard solution, specifically see Table 2:

[0033] Table 2 Precision and accuracy table of low concentration and medium concentration ammonia nitrogen standard solution

[0034]

Embodiment 3

[0035] Embodiment 3: determination of detection limit, see table 3:

[0036] Table 3 Determination of detection limits

[0037]

[0038] Detection limit (mg / L) calculation: DL = SD×t(n-1,0.01), where: t(n-1,0.01) is the one-sided significance level, t is 3.143,

[0039] DL = SD×t(n-1,0.01) =0.001852×3.143 =0.005821≈0.006mg / L

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Abstract

The invention discloses a rapid salicylate spectrophotometric assay method for ammonia nitrogen in water. The rapid salicylate spectrophotometric assay method is characterized in that a centrifuge is utilized for centrifugal separation of a water sample subjected to flocculation and sedimentation to remove flocculent precipitates in the water sample, then water-bath heating is utilized to improve the chemical equilibrium constants of ammonia nitrogen reaction, and finally, the ultraviolet spectrophotometry is adopted for detection. The rapid salicylate spectrophotometric assay method speeds up reaction, reduces interference of flocculates, enables follow-up detection to be accurate and reliable, is high in flexibility and excellent in recovery rate and precision, and therefore, can finish detection more quickly on the premise of guaranteeing the accuracy.

Description

technical field [0001] The invention relates to a method for measuring ammonia nitrogen content in drinking water, in particular to a method for rapidly detecting ammonia nitrogen in water by a salicylate spectrophotometric method. Background technique [0002] Ammonia nitrogen is an important indicator of the quality of drinking water. As an important indicator of daily water quality testing in water supply enterprises, how to detect the content of ammonia nitrogen in water more safely, environmentally friendly and quickly has always been a difficult problem in the water quality testing industry. For example, when using Nessler's reagent method to detect ammonia nitrogen content in raw water, although it is simple and easy to implement, Nessler's reagent has relatively high toxicity and is likely to cause pollution to the environment. Metal ions and suspended solids such as calcium, magnesium and iron in water will interfere with the determination, so corresponding pretreat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N21/78
CPCG01N21/31G01N21/78
Inventor 费相琴贺威陈艳芬吴爽罗维红张亚敏
Owner 淮安自来水有限公司
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