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System and method for emulsion breaking

A technology of latex and dispersed phase, which is applied in the field of decomposing latex systems, and can solve problems affecting genetic testing methods and disadvantages

Active Publication Date: 2016-11-09
LIFE TECH CORP
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  • Abstract
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  • Claims
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Problems solved by technology

Such yield losses can adversely affect genetic testing methods

Method used

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  • System and method for emulsion breaking
  • System and method for emulsion breaking
  • System and method for emulsion breaking

Examples

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Embodiment Construction

[0017] In an exemplary embodiment, a method of recovering a bead support from latex comprises applying an aqueous surfactant solution to a centrifuge tube. Apply the hydrophobic liquid over the surfactant solution in the centrifuge tube. In one example, the ratio of the volume of the aqueous surfactant solution to the volume of the hydrophobic liquid is not greater than 1.0, such as not greater than 0.5. Alternatively, the ratio of the initial volume of the aqueous surfactant solution to the total volume of the centrifuge tube is not greater than 0.5, such as not greater than 0.2 or not greater than 0.1. The centrifuge tubes can be located within the centrifuge rotor. Latex can be applied over the hydrophobic liquid while spinning the centrifuge rotor containing the centrifuge tube. The latex comprises a dispersed aqueous phase comprising a bead support. As the latex decomposes, the material comprising the dispersed phase of the bead support can preferentially partition int...

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Abstract

A method of recovering a bead support from an emulsion includes supplying an aqueous surfactant solution into a centrifuge tube; supplying a hydrophobic liquid over the surfactant solution in the centrifuge tube, wherein a ratio of the volume of the aqueous surfactant solution to the volume of the hydrophobic liquid is not greater than 0.5; and applying an emulsion over the hydrophobic liquid while centrifuging, the emulsion comprising a dispersed aqueous phase including the bead support, the emulsion breaking and material of the dispersed phase preferentially partitioning to the surfactant solution.

Description

[0001] Cross References to Related Applications [0002] This application claims the benefit of US Provisional Application No. 61 / 902,897, filed November 12, 2013, which is hereby incorporated by reference in its entirety. [0003] This application claims the benefit of US Provisional Application No. 61 / 942,167, filed February 20, 2014, which is hereby incorporated by reference in its entirety. technical field [0004] The present invention generally relates to systems and methods for breaking down latex. Background technique [0005] Latex is used in a range of industries, including the food, bioscience and chemical industries. Specifically, the chemical and bioscience industries utilize latex to isolate a large number of reactants. For example, the chemical industry utilizes latex for emulsion polymerization, in which a small amount of monomer solution is isolated into a dispersed phase and subsequently polymerized. In biological sciences, and specifically in genetic s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6806C07H1/06C07H21/00C12Q2527/125C12Q2527/137C12Q2563/159B01D17/04B01D17/041B01D17/047
Inventor B.里德J.鲍尔
Owner LIFE TECH CORP
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