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Systems and methods for emulsion breakdown

An emulsion and liquid technology, applied in the field of decomposing emulsion systems, can solve problems such as unfavorable and affecting genetic testing methods

Active Publication Date: 2020-04-07
LIFE TECH CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Such yield losses can adversely affect genetic testing methods

Method used

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  • Systems and methods for emulsion breakdown
  • Systems and methods for emulsion breakdown
  • Systems and methods for emulsion breakdown

Examples

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Embodiment Construction

[0017] In an exemplary embodiment, a method of recovering a bead support from an emulsion comprises applying an aqueous surfactant solution to a centrifuge tube. Apply the hydrophobic liquid over the surfactant solution in the centrifuge tube. In one example, the ratio of the volume of the aqueous surfactant solution to the volume of the hydrophobic liquid is not greater than 1.0, such as not greater than 0.5. Alternatively, the ratio of the initial volume of the aqueous surfactant solution to the total volume of the centrifuge tube is not greater than 0.5, such as not greater than 0.2 or not greater than 0.1. The centrifuge tubes can be located within the centrifuge rotor. Emulsions can be applied over hydrophobic liquids while spinning the centrifuge rotor containing the centrifuge tubes. The emulsion comprises a dispersed aqueous phase comprising a bead support. Upon disintegration of the emulsion, the material comprising the dispersed phase of the bead support can prefe...

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Abstract

A method of recovering a bead support from an emulsion comprising: supplying an aqueous surfactant solution into a centrifuge tube; supplying a hydrophobic liquid over the surfactant solution in the centrifuge tube, wherein the surfactant the ratio of the volume of the aqueous solution to the volume of the hydrophobic liquid is no greater than 0.5; and upon centrifugation, an emulsion is applied on the hydrophobic liquid, the emulsion comprising a dispersed aqueous phase comprising the bead support, the The emulsion breaks down and the material of the dispersed phase preferentially separates into the surfactant solution.

Description

[0001] Cross References to Related Applications [0002] This application claims the benefit of US Provisional Application No. 61 / 902,897, filed November 12, 2013, which is hereby incorporated by reference in its entirety. [0003] This application claims the benefit of US Provisional Application No. 61 / 942,167, filed February 20, 2014, which is hereby incorporated by reference in its entirety. technical field [0004] The present invention generally relates to systems and methods for breaking up emulsions. Background technique [0005] Emulsions are used in an industrial range including the food, bioscience and chemical industries. Specifically, the chemical and bioscience industries utilize emulsions to isolate large quantities of reactants. For example, the chemical industry utilizes emulsions for emulsion polymerization, where a small amount of monomer solution is isolated into a dispersed phase and subsequently polymerized. In the biological sciences, and specifical...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6806C07H1/06C07H21/00C12Q2527/125C12Q2527/137C12Q2563/159B01D17/04B01D17/041B01D17/047
Inventor B.里德J.鲍尔
Owner LIFE TECH CORP