Preparation method of nitrosohemoglobin pigment with thermal stability

Abstract

The invention discloses a preparation method of nitrosohemoglobin pigment with thermal stability, belonging to the technical field of preparation of hemoglobin pigment. The preparation method of nitrosohemoglobin pigment with thermal stability comprises the following steps: mixing sodium citrate and pig blood; filtering the mixture; carrying out ice bath processing to collect hemocytes; carrying out oscillating reaction on the hemocytes and deionized water to obtain hemocyte broken mixed liquid; mixing the hemocyte broken mixed liquid with D-sodium isoascorbate, ammonium nitrite and glycerol to prepare hemoglobin mixed liquid; heating the hemoglobin mixed liquid and specially-made hydrolytic bagasse gel liquid in a water bath; carrying out centrifugation, and then collecting supernatant liquid; and carrying out vacuum freeze drying so as to obtain the nitrosohemoglobin pigment with thermal stability. Embodiments prove that the preparation method has the advantages that the processes are simple and convenient, and the reaction is mild; animal blood by-products are effectively utilized, so that wastes are changed into valuables and environmental pollution is reduced; and meanwhile, the prepared nitrosohemoglobin pigment has stable color and luster and is not easy to fade, and the thermal stability and dispersibility of the nitrosohemoglobin pigment are obviously improved. The preparation method is suitable for industrial production.

Description

technical field [0001] The invention discloses a preparation method of heat-stable nitrosohemoglobin pigment, which belongs to the technical field of hemoglobin pigment preparation. Background technique [0002] my country's meat food industry is an emerging industry developed since the reform and opening up, and occupies an important position in the food industry. According to statistics, my country's total meat output has reached 62 million tons per year, ranking first in the world and accounting for the world's meat. 28.9% of the total. At present, the average meat production in the world accounts for 10% to 20% of the meat production, and the meat production in some developed countries accounts for 60% to 70% of the total meat production, but there is still a big gap between my country and this ratio. There are only more than 2 million tons of deep-processed meat products in my country every year, accounting for 4% of the total meat. Therefore, my country's meat processi...

AI Technical Summary

Problems solved by technology

[0005] The technical problem mainly solved by the present invention: aiming at the poor thermal stability, unstable color and easy fading of currently commonly used nitrosohemoglobin, if embedded in microcapsules, the stability is still not improved much, and the dispersibility is still poor. Provided is a mixture of sodium citrate and pig blood, filtered, treated in an ice bath to collect blood cells, oscillating with deionized water to obtain a blood cell broken mixture, mixed with D-sodium erythorbate, ammonium nitrite, and glycerin to prepare The hemoglobin mixture is heated in a water bath with a specially made hydrolyzed bagasse gel solution, the supernatant is collected after centrifugation, and vacuum freeze-dried to obtain a heat-stable nitrosohemoglobin pigment.