Cobalt tetroxide microbelt with high peroxidase-like activity and stability and preparation method thereof

A technology of peroxidase and microribbons, applied in the direction of catalyst activation/preparation, metal/metal oxide/metal hydroxide catalysts, chemical instruments and methods, etc. Reduce activity and other problems, achieve high peroxidase-like activity and stability, high product purity, and low cost

Active Publication Date: 2019-04-05
山东中酶生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, when these materials are used as simulated enzymes, there are the following problems: (1) the preparation process of high-quality nanomaterials is usually complicated; (2) the instability of nanomaterials itself leads to oxidation or agglomeration of the particle surface during use, resulting in Catalytic activity decreases; (3) After the catalyst is used, it is usually difficult to recycle
The existence of these problems limits its practical application

Method used

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  • Cobalt tetroxide microbelt with high peroxidase-like activity and stability and preparation method thereof
  • Cobalt tetroxide microbelt with high peroxidase-like activity and stability and preparation method thereof
  • Cobalt tetroxide microbelt with high peroxidase-like activity and stability and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Weigh 1.0 g of polyvinylpyrrolidone (PVP), and dissolve it in 10 mL of ethanol under magnetic stirring. middle. After the PVP is completely dissolved, add 2mL cobalt nitrate solution (containing 0.45g Co(NO 3 ) 2 ·6H 2 O). When the sol is well mixed, transfer it to the syringe. Set the voltage of the electrospinning machine to 20kV, the receiving distance to 20cm, and the pump speed to 0.002mm / s. After the electrospinning was completed, the samples were collected and dried in an oven at 80 °C for at least 12 h. Finally, the final sample was calcined, and the calcination conditions were as follows: the room temperature was raised to 300°C at 1°C / min, and kept for 1h; then the temperature was raised to 400°C at 0.5°C / min, and kept for 2h.

Embodiment 2

[0039] Weigh 1.0 g of polyvinylpyrrolidone (PVP), and dissolve it in 8 mL of ethanol under magnetic stirring. middle. After the PVP is completely dissolved, add 2mL cobalt nitrate solution (containing 0.24g Co(NO3 ) 2 ·6H 2 O). When the sol is well mixed, transfer it to the syringe. Set the voltage of the electrospinning machine to 18kV, the receiving distance to 15cm, and the pumping speed to 0.003mm / s. After the electrospinning was completed, the samples were collected and dried in an oven at 70 °C for at least 12 h. Finally, the final sample was calcined, and the calcining conditions were as follows: room temperature was raised to 250°C at 0.8°C / min and kept for 1.5h; then raised to 350°C at 0.2°C / min and kept for 2.5h.

Embodiment 3

[0041] Weigh 1.0 g of polyvinylpyrrolidone (PVP), and dissolve it in 12 mL of ethanol under magnetic stirring. middle. After the PVP is completely dissolved, add 2mL cobalt nitrate solution (containing 0.57g Co(NO 3 ) 2 ·6H 2 O). When the sol is well mixed, transfer it to the syringe. Set the voltage of the electrospinning machine to 22kV, the receiving distance to 25cm, and the pumping speed to 0.005mm / s. After the electrospinning was completed, the samples were collected and dried in an oven at 90 °C for at least 12 h. Finally, the final sample was calcined. The calcination conditions were as follows: the room temperature was raised to 350°C at 3°C / min and kept for 0.5h; then the temperature was raised to 450°C at 0.8°C / min and kept for 1.5h.

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Abstract

The invention discloses a cobaltosic oxide micro-ribbon with high peroxidase-like activity and stability and a preparation method therefor. According to the Co3O4 micro-ribbon with high peroxidase-like activity and stability, the micro-ribbon is of a ribbon-shaped structure formed through connecting Co3O4 single-crystal grains. The preparation method comprises the steps: firstly, dissolving polyvinyl pyrrolidone (PVP) into ethanol, adding a cobalt nitrate solution into the solution after the PVP is completely dissolved, and carrying out uniform mixing, so as to obtain spinning sol; then, carrying out spinning on the spinning sol by adopting an electrostatic spinning method, so as to obtain gelatinous fibers, and carrying out drying; and finally, carrying out staged calcination on the dried gelatinous fibers, thereby obtaining the Co3O4 micro-ribbon. When the micro-ribbon is used as mimetic peroxidase, activity can be shown in a relatively wide pH and temperature range; a sample shows relatively good affinity to H2O2 and TMB; and meanwhile, the micro-ribbon with relatively high specific surface area shows relatively high peroxidase-like activity and stability.

Description

technical field [0001] The invention belongs to the technical field of inorganic materials, in particular to a Co 3 o 4 Microribbons and methods of making them. Background technique [0002] Peroxidase is a kind of oxidoreductase that widely exists in living organisms. In the process of life activities, it is mainly involved in catalyzing the decomposition of peroxides in living organisms. It is one of the key enzymes in the biological defense system. The disadvantages of natural peroxidase, such as complex structure, high price, heat sensitivity, poor stability and limited sources, limit its large-scale development and utilization. The widely used peroxidase is horseradish peroxidase (horseradish peroxidase, HRP) extracted from natural plants, but HRP is expensive, and the storage and use conditions are harsh, and it is easy to inactivate. The simulated enzyme has a simpler structure than the natural enzyme, stable chemical properties, catalytic activity of the enzyme, h...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J23/75B01J35/02B01J35/10B01J37/34B01J37/08G01N21/31
CPCB01J23/75B01J35/023B01J35/10B01J37/08B01J37/342G01N21/31
Inventor 孙海燕刘小健张志良陶芙蓉张华勇
Owner 山东中酶生物科技有限公司
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