Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

CTL epitope peptide of tumor antigen PSA (prostate specific antigen) and application of CTL epitope peptide

A tumor antigen and epitope peptide technology, applied in the field of epitope peptides, can solve the problem of lack of CTL epitope peptide antigen, and achieve the effect of high tissue and organ specificity

Inactive Publication Date: 2016-11-16
SINOBIOWAY CELL THERAPY CO LTD
View PDF10 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, at present, the antigens for inducing specific DC-CIK cells still need to be improved, and there is a lack of CTL epitope peptide antigens that can efficiently stimulate tumor-specific DC-CIK

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • CTL epitope peptide of tumor antigen PSA (prostate specific antigen) and application of CTL epitope peptide
  • CTL epitope peptide of tumor antigen PSA (prostate specific antigen) and application of CTL epitope peptide
  • CTL epitope peptide of tumor antigen PSA (prostate specific antigen) and application of CTL epitope peptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: Synthetic Epitope Peptides

[0027] Using the method of combining theory and practice, according to the primary structure of the antigen, comprehensively using immunoinformatics means, using SYFPEITHI, BIMAS, NetCTL, WAPP and EpiJen to comprehensively predict and analyze the antigen CTL epitopes of PSA, and select the top 20 scores The polypeptide sequences were tested and screened, and they were named P1, P2, P3...P20 at a time.

[0028] The basic process is as follows: first, an amino acid whose amino group is protected by an Fmoc group is connected to an insoluble solid-phase carrier Wang resin, and then the protecting group of the amino group is removed, and the first amino acid is connected to the solid-phase carrier; secondly, the second The amino group is activated by the carboxyl condensing agent of the amino acid protected by the Fmoc group, and the activated second amino acid carboxyl reacts with the amino group of the first amino acid that has been...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an anti-tumor CTL epitope peptide derived from PSA (prostate specific antigen), wherein the epitope peptide is nonapeptide, and the amino acid sequence of the nonapeptide is shown as Cys-Ala-Leu-Pro-Glu-Arg-Pro-Ser-Leu. The invention also discloses an application of the peptide in preparing tumor therapeutic DC-CIK. The specific DC-CIK cells prepared by the invention have a certain killing rate on prostate cancer cells LNCap; and the specific DC-CIK cells can be used for preparing prostate cancer specific autoimmune cells.

Description

technical field [0001] The present invention relates to the technical field of epitope peptides, in particular to a CTL recognition epitope peptide of specific tumor antigen PSA and its application, and also to a preparation method and application of tumor antigen PSA-specific DC cells, and a tumor antigen PSA-specific epitope peptide and its application. Preparation method and application of antigen PSA-specific DC-CIK cells. Background technique [0002] Prostate Specific Antigen (PSA) is a single-chain glycoprotein secreted by the epithelial cells of the prostate acini and ducts. It is functionally a serine protease similar to kallikrein and participates in the liquefaction process of semen. PSA is a specific marker of prostate cancer. PSA is a prostate specific antigen, and its diagnostic specificity for prostate cancer is 90-97%. It is considered to be the most valuable prostate cancer tumor marker, and is widely used in the screening, diagnosis and post-treatment moni...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N9/64C12N5/0784C12N5/0783A61K39/00A61K35/15A61K35/17A61P35/00
CPCC12N9/6424A61K35/15A61K35/17A61K39/0011C12N5/0638C12N5/0639C12Y304/21106A61K2300/00
Inventor 唐奇冯振卿许国贞刘振云朱进唐小军蒯兴旺
Owner SINOBIOWAY CELL THERAPY CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com