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Application of LY-6 (Rhodopseudomonas palustris LY-6) biocontrol agent in control of rice blast

A technology of swamp rhodopseudomonas and biocontrol agents, which is applied in the field of biopesticides to achieve the effects of simple production process, low cost and short cultivation time

Active Publication Date: 2016-11-23
HUNAN PLANT PROTECTION INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the photosynthetic bacterium Rhodopseudomonas palustris has not been reported in the prevention and control of rice blast

Method used

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  • Application of LY-6 (Rhodopseudomonas palustris LY-6) biocontrol agent in control of rice blast
  • Application of LY-6 (Rhodopseudomonas palustris LY-6) biocontrol agent in control of rice blast

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A preparation method of Rhodopseudomonas palustris LY-6 biocontrol agent of the present invention, comprises the following steps:

[0029] (1) Activation: Culture the preserved species of Rhodopseudomonas palustris LY-6 strain according to the double-layer plate culture method until red single colonies appear; the medium used is solid medium for photosynthetic bacteria, and the formula is: ( NH 4 ) 2 SO 4 0.1 wt%, MgSO 4 0.02 wt%, NaHCO 3 0.5 wt%, K 2 HPO 4 0.05 wt%, NaCl 0.02 wt%, yeast extract 0.15 wt% and agar 1.5 wt%, pH=7.0.

[0030] (2) Seed culture: Inoculate the red single colony obtained in step (1) into a 120 mL serum bottle. Use photosynthetic bacteria liquid medium to cultivate to the logarithmic growth phase; the culture medium adopted is photosynthetic bacteria liquid medium, and the formula is: (NH 4 ) 2 SO 4 0.1 wt%, MgSO 4 0.02wt%, NaHCO 3 0.5 wt%, K 2 HPO 4 0.05 wt%, NaCl 0.02 wt%, yeast extract 0.15 wt% (all mass fractions), pH=7...

Embodiment 2

[0034] Example 2: Prevention and control of leaf blast in the field with Rhodopseudomonas palustris LY-6 biocontrol agent

[0035] The biocontrol agent (concentration 5 × 10 9 cfu / mL), the inactivated biocontrol agent prepared in Comparative Example 1, and chemical pesticides (Blafling, 120 mL / mu) were sprayed on the rice leaves at the late tillering stage. Three replicates were set up for each treatment, and each 20 L per mu, sprayed once every 15 days, and applied 3 times in total. Fifteen days after the last pesticide application, the rice leaf disease index was calculated by sampling according to the 5-point method, and the respective control effects were calculated.

[0036] figure 1 It is a comparison chart of the control effects of the inactivated biocontrol agent of Comparative Example 1, the biocontrol agent of Example 1, and chemical pesticides on rice leaf blast. A and B represent significant differences (P figure 1 It can be seen in the figure that the control ef...

Embodiment 3

[0037] Example 3: Prevention and control of panicle blast in the field with Rhodopseudomonas palustris LY-6 biocontrol agent

[0038] The biocontrol agent (concentration 5 × 10 9 cfu / mL), inactivated biocontrol agents and chemical pesticides prepared in Comparative Example 1 (Blastin, 120 mL / mu) were sprayed on the rice leaves at the heading stage, and each treatment was repeated 3 times. Mu 20 L, sprayed once every 15 days, a total of 2 times of application. After 15 days, the rice panicle disease index was calculated by sampling according to the 5-point method, and the respective control effects were calculated.

[0039] figure 2 It is a comparison chart of the control effects of the inactivated biocontrol agent of Comparative Example 1, the biocontrol agent of Example 1, and chemical pesticides on rice panicle blast. A and B represent significant differences (P<0.01). The control effects of applying inactivated biocontrol agents, biocontrol agents and pesticides are 11...

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Abstract

The invention discloses an application of LY-6 (Rhodopseudomonas palustris LY-6) biocontrol agent in control of rice blast. The LY-6 biocontrol agent is obtained through cultivation of LY-6. LY-6 is applied to control of rice blast for the first time, and the LY-6 biocontrol agent has characteristics of being environment-friendly, non-toxic to humans and animals, phytotoxicity-free to crops, simple and convenient to apply and the like.

Description

technical field [0001] The invention relates to the technical field of biological pesticides, in particular to the application of a rhodopseudomonas palustris biocontrol agent in preventing and controlling rice blast. Background technique [0002] Rice blast is one of the important diseases of rice. The disease is widely distributed. It occurs in more than 80 countries and regions in the world, and it also occurs in the north and south rice regions of my country. In areas where the disease is endemic, the yield is generally reduced by 10% to 20%, and in severe cases it can reach 40% to 50%. Especially heavy fields may even fail to harvest. For the control of rice blast, chemical control is still the main method at present, but due to the increase of drug resistance and untimely discovery, the application of pesticides is extremely unscientific and reasonable, and the dosage and frequency of pesticide applications seriously exceed the standard, causing major problems such as ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/00A01P3/00
CPCA01N63/00
Inventor 刘勇张德咏陈岳谭新球苏品肖友伦李小娟
Owner HUNAN PLANT PROTECTION INST
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