A kind of clarification method of green tea soup
A green tea and tea soup technology, applied in the field of tea soup clarification, can solve the problems that green tea beverages are unfavorable to the healthy development of tea beverage products, achieve good market prospects, small changes in main components, and improve clarity
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Embodiment 1
[0026] For implementing the present invention, the applicant has preserved this Aspergillus niger strain in China General Microbiological Culture Collection Center (China General Microbiological Culture Collection Center, CGMCC) on August 26, 2015. The preservation number of this Aspergillus niger strain is : CGMCC No.11276, Address: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing.
[0027] The Aspergillus niger strain of the present invention is derived from instant green tea powder that has been placed at room temperature for 1 year. The specific separation method is as follows: in an ultra-clean workbench, take an appropriate amount of instant green tea powder that has been stored for 1 year and dissolve it in 100 mL of sterile water, and place it at a temperature of 37 ° C. After cultivating in a shaker with a rotation speed of 150r / min for 4 days, pick the mycelium in the culture solution and inoculate it on a PDA plate; place the PDA plate inoculated with myc...
Embodiment 2
[0044] A method for clarifying green tea soup, specifically comprising the steps of:
[0045] 1. The green tea powder is fully dissolved in deionized water at 50°C at a ratio of 1:15 by mass of tea to water to obtain green tea soup, which is sterilized at 105°C for 8 minutes and cooled to 37°C.
[0046] 2. Inoculate the Aspergillus niger strain with the preservation number CGMCC No.11276 on the PDA medium under the condition of 4°C, cultivate and activate it at a constant temperature at 37°C for 3 days to obtain the Aspergillus niger spore liquid;
[0047] 3. Mix green tea soup and Aspergillus niger spore liquid, green tea tea soup: Aspergillus niger spore liquid (v / v)=25:1, cultivate 24 hours in a constant temperature shaker at a temperature of 37±3°C and a rotation speed of 150r / min to obtain black tea Aspergillus seed liquid. And adjust the Aspergillus niger spore concentration in the Aspergillus niger seed liquid to be 2.0×10 7 CFU / mL.
[0048] 4. Take 100mL of green te...
Embodiment 3
[0051] A method for clarifying green tea soup, specifically comprising the steps of:
[0052] 1. The green tea powder is fully dissolved in deionized water at 90°C at a ratio of 1:35 by mass of tea to water to obtain green tea soup, which is sterilized at 80°C for 25 minutes and cooled to 40°C.
[0053] 2. Inoculate the Aspergillus niger strain with the preservation number of CGMCC No. 11276 on the PDA medium at 4°C, cultivate and activate it at a constant temperature at 37°C for 3 days to obtain the Aspergillus niger spore liquid.
[0054] 3. Preparation of Aspergillus niger seed liquid: mix green tea soup with Aspergillus niger spore liquid, wherein, green tea tea soup: Aspergillus niger spore liquid (v / v) = 50:1, and place at a temperature of 37 ± 3 ° C, shaker revolutions Under the condition of 150r / min, aerated culture was carried out for 24 hours to obtain Aspergillus niger seed liquid. And adjust the spore concentration of Aspergillus niger seed solution to 0.5×10 7 C...
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