Multi-column quadrature-axis countercurrent chromatograph

Abstract

The invention discloses a multi-column quadrature-axis countercurrent chromatograph. The multi-column quadrature-axis countercurrent chromatograph comprises a machine case, a support frame, a separation column assembly and a motor, wherein the support frame is mounted in the machine case, and the motor drives the support frame to rotate along the horizontal direction through a first transmission mechanism; the separation column assembly comprises four separation columns which are roughly symmetrically distributed on the left and right sides of the support frame, and the rotating directions of the two separation columns on the same side are different; and the rotating directions of the two separation columns on a cross position of different side are the same. According to the multi-column quadrature-axis countercurrent chromatograph, the unwinding of a flexible pipe is realized by virtue of a method of replacing an unwinding shaft with the separation columns, the rotating directions of the separation columns are differed by virtue of the transmission mechanism, and an unwinding pipe is independently unwound, so that the unwinding reliability is guaranteed; and the separation columns are mounted on the support frame through flange bearings and can be changed by detaching the flange bearings mounted on two ends of the separation columns, and therefore, the different types of the separation columns can be conveniently changed.

Description

technical field [0001] The invention relates to an orthogonal-axis countercurrent chromatograph using liquid-liquid extraction as a separation and distribution principle applied in the field of biochemistry, in particular to a multi-column orthogonal-axis countercurrent chromatograph. Background technique [0002] Orthogonal axis countercurrent chromatograph is a kind of countercurrent chromatograph. It is different from the common J-type countercurrent chromatograph. The rotation axis of the separation column of the orthogonal axis countercurrent chromatograph and the revolution axis of the instrument are orthogonal to each other at an angle of 90 degrees. Because this special structure can form a three-dimensional asymmetric centrifugal force field, the hydrodynamic state of the two phases in the separation column shows a special form, which is very conducive to the retention of the stationary phase of the hydrophilic solvent system, and is suitable for biological large Is...

AI Technical Summary

Problems solved by technology

[0006] In the technical scheme of the patent (application number: 97202779.3, patent name: countercurrent chromatograph), the countercurrent chromatograph belongs to the XL type of orthogonal axis countercurrent chromatography, and its design shows the structural characteristics of a typical orthogonal axis countercurrent chromatograph. However, there is still room for improvement in the design structure

For example, the unwinding method is simultaneous unwinding of multiple tubes. This unwinding method is prone to intertwining during the unwinding process due to the different degrees of fatigue of different pipelines.

For another example, this technology makes it difficult to replace the separation column due to the separation column drawn out by double tubes; for another example, this model is XL type, and it is impossible to collect X-type experimental data on it.

Images