Preparation method of marine active calcium containing beneficial bacteria
A technology of beneficial bacteria and active calcium, which is applied in the field of preparation of marine active calcium, can solve the problems of ineffective use and destruction, and achieve the effect of improving the body's immunity and enriching active calcium components
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Embodiment 1
[0026] The preparation method of the marine active calcium that contains beneficial bacteria of the present embodiment comprises the following steps:
[0027] (1) By weight, get 0.02 part of corn flour, 0.02 part of bean flour, 0.01 part of NAMn medium, 5 parts of fineness of 80 orders, 40% solid content of snail shell slurry, 2 parts of fineness of 80 mesh, cyanophyta algae slurry with a solid content of 20% and 100 parts of water, mixed evenly for later use;
[0028] (2) Add the mixture obtained in step (1) into a fermenter, seal it tightly, and feed in steam sterilized by filtration, maintain the temperature at 121-123° C., and sterilize under pressure at 0.11 MPa for 0.5 hours;
[0029] (3) Cool down to below 30°C, insert Bacillus subtilis at a ratio of 3% of the inoculum, heat up to 30°C for liquid-phase fermentation, during the fermentation process, detect the fermentation situation, and stop the fermentation when the spore rate reaches more than 90%;
[0030] (4) Dryin...
Embodiment 2
[0033] The preparation method of the marine active calcium that contains beneficial bacteria of the present embodiment comprises the following steps:
[0034] (1) By weight, get 0.5 part of corn flour, 0.3 part of bean flour, 0.05 part of NAMn medium, 25 parts of chiton shell slurry whose fineness is 100 mesh, solid content of 60%, 20 parts of fineness Protochlorophyta algae slurry of 100 mesh, 60% solid content and 100 parts of water, mixed evenly for later use;
[0035] (2) Add the mixture obtained in step (1) into the fermenter, seal it tightly, and feed it with filtered and sterilized steam, maintain the temperature at 122-124° C., and sterilize under pressure at 0.11 MPa for 2 hours;
[0036] (3) Cooling down to below 30°C, inserting Bacillus licheniformis in proportion to 10% of the inoculum amount, raising the temperature to 45°C for liquid-phase fermentation, during the fermentation process, detecting the fermentation situation, and stopping the fermentation when the s...
Embodiment 3
[0039] Embodiment 3: the preparation method of the marine active calcium containing beneficial bacteria of the present embodiment comprises the following steps:
[0040] (1) By weight, get 0.1 part of corn flour, 0.3 part of bean flour, 0.03 part of NAMn medium, 10 parts of fineness of 80 mesh, 50% solid content of oyster shell slurry, 15 parts of fineness of 100 mesh, Rhodophyta algae slurry with a solid content of 40% and 100 parts of water, mixed evenly for subsequent use;
[0041] (2) Add the mixture obtained in step (1) into the fermenter, seal it tightly, feed in steam sterilized by filtration, maintain the temperature at 123-125°C, and sterilize under pressure at 0.11MPa for 1 hour;
[0042](3) Cooling down to below 30°C, inserting Bacillus subtilis and Bacillus licheniformis with a quality of 1:1 at a ratio of 5% of the inoculum amount, raising the temperature to 40°C for liquid phase fermentation, during the fermentation process, detecting the fermentation situation, ...
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