Preparation method of marine active calcium containing beneficial bacteria

A technology of beneficial bacteria and active calcium, which is applied in the field of preparation of marine active calcium, can solve the problems of ineffective use and destruction, and achieve the effect of improving the body's immunity and enriching active calcium components

Inactive Publication Date: 2016-11-30
NINGBO JIANGDONG HUIZE BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these beneficial substances are completely oxidized and destroye

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0025] Example 1:

[0026] The preparation method of marine active calcium containing beneficial bacteria of this embodiment includes the following steps:

[0027] (1) By weight, take 0.02 parts of corn flour, 0.02 parts of soybean flour, 0.01 parts of NAMn medium, 5 parts of snail shell paste with 80 mesh fineness and 40% solid content, and 2 parts fineness as 80 mesh, 20% solid content of Cyanophyta algae slurry and 100 parts of water, mix well for use;

[0028] (2) Add the mixture obtained in step (1) to the fermentor, seal it, and pass in filtered and sterilized steam, maintain the temperature at 121-123°C, and sterilize under pressure at 0.11MPa for 0.5 hours;

[0029] (3) Cool down to below 30°C, insert Bacillus subtilis in proportion of 3% of the inoculum, and heat up to 30°C for liquid phase fermentation. During the fermentation process, check the fermentation condition and stop the fermentation when the spore rate reaches 90% or more;

[0030] (4) Drying by spray drying, the o...

Example Embodiment

[0032] Example 2:

[0033] The preparation method of marine active calcium containing beneficial bacteria of this embodiment includes the following steps:

[0034] (1) By weight, take 0.5 part of corn flour, 0.3 part of soybean flour, 0.05 part of NAMn medium, 25 parts of stone turtle shell slurry with a fineness of 100 mesh and a solid content of 60%, and 20 parts of fineness It is 100 meshes, 60% solid content of Prochlorophyta algae slurry and 100 parts of water, mix well for use;

[0035] (2) Put the mixture obtained in step (1) into the fermentation tank, airtight it, and pass in filtered and sterilized steam, maintain the temperature at 122-124°C, and sterilize under pressure at 0.11MPa for 2 hours;

[0036] (3) Cool down to below 30°C, insert Bacillus licheniformis at a rate of 10% of the inoculum, and raise the temperature to 45°C for liquid phase fermentation. During the fermentation process, check the fermentation condition and stop the fermentation when the spore rate reach...

Example Embodiment

[0039] Embodiment 3: The preparation method of marine active calcium containing beneficial bacteria of this embodiment includes the following steps:

[0040] (1) By weight, take 0.1 part of corn flour, 0.3 part of soybean flour, 0.03 part of NAMn medium, 10 parts of oyster shell slurry with 80 mesh fineness and 50% solid content, 15 parts of fineness as 100 meshes, 40% solid content of Rhodophyta algae slurry and 100 parts of water, mix well for use;

[0041] (2) Add the mixture obtained in step (1) to the fermentation tank, airtightly, and pass in filtered and sterilized steam, maintain the temperature at 123~125°C, and sterilize under pressure at 0.11MPa for 1 hour;

[0042] (3) Cool down to below 30°C, insert Bacillus subtilis and Bacillus licheniformis with a mass of 1:1 at the ratio of 5% of the inoculum, and raise the temperature to 40°C for liquid phase fermentation. During the fermentation process, check the fermentation status. Stop fermentation when the rate reaches 90% or...

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Abstract

The invention relates to a preparation method of marine active calcium containing beneficial bacteria. According to the method, starch, a NAMn culture medium, shellfish and seaweed marine plants are mixed and undergo high-temperature disinfection and sterilization, the mixture is inoculated with beneficial bacteria, aerobic liquid-phase fermentation is carried out to convert calcium in the shellfish powder to marine active calcium while seaweed marine plants are degraded into active materials such as alginic acid, glucosamine oligosaccharide, organic polypeptide, amino acid and the like with the fermentation process and various medium and trace elements enriched in marine organisms and shells are released; and finally, the product is dried to obtain the marine active calcium containing beneficial bacteria. According to the method, without high-temperature calcination, natural organic active calcium and substances with effects of resisting virus, resisting bacteria and enhancing body immunity in shellfish marine species are maintained to the maximum. Through the liquid-phase fermentation technology, macromolecular organic matter or high-molecular substances in shellfish marine species are degraded into micromolecules, which are beneficial to digestion and absorption in animals or plants.

Description

technical field [0001] The invention relates to the technical field of preparation of active calcium, in particular to a preparation method of marine active calcium containing beneficial bacteria. Background technique [0002] Biological microecological preparations are a type of biological products that prevent and treat intestinal diseases of animals, induce plant resistance and reduce disease occurrence, and have been widely used in livestock and poultry breeding and plant pest control. Calcium deficiency in animals and plants is also one of the important factors affecting the growth of animals and plants. When calcium is deficient, cell division cannot be carried out or completed normally. For example, postpartum paralysis caused by calcium deficiency in dairy cows, calcium-deficient diet, etc. will cause bone calcium loss , bone and joint damage, etc.; when the plant is deficient in calcium, the front end of the root will turn brown, the branches and leaves will become ...

Claims

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Application Information

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IPC IPC(8): A23K10/12A23K20/24A23K20/147A23K20/142A23K20/121A23K20/163A23K20/20C05D5/00C05G3/00C05G3/04C12P1/04C12R1/125C12R1/10C05G3/80
CPCC05D5/00C05G3/00C12P1/04C05G3/80C12N1/205C12R2001/10C12R2001/125
Inventor 孟秋峰韩慧聪潘龙琳
Owner NINGBO JIANGDONG HUIZE BIOLOGICAL TECH CO LTD
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