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A method for establishing a mouse model of spinal arthritis based on conditional knockout technology and its application

A spondyloarthritis, mouse model technology, applied in pharmaceutical formulations, preparations for in vivo experiments, compound screening/testing, etc., can solve problems such as large differences in pathological characteristics, unstable modeling, and achieve model reproduction Good performance and simple operation

Active Publication Date: 2019-04-16
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] The present invention mainly solves the problems existing in the prior art such as unstable modeling and large differences between pathological features and clinical diseases, and provides a new conditional knockout technique with a clear genetic background and closer to the true symptoms of the disease. Method for Pathological Model of Spondyloarthritis

Method used

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  • A method for establishing a mouse model of spinal arthritis based on conditional knockout technology and its application
  • A method for establishing a mouse model of spinal arthritis based on conditional knockout technology and its application
  • A method for establishing a mouse model of spinal arthritis based on conditional knockout technology and its application

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Embodiment 1

[0034] A method for establishing a mouse model of spinal arthritis based on conditional knockout technology is implemented in the following steps:

[0035] Step 1, breeding of mice: mix SHP-2 flox / flox Transgenic mice and CD4-Cre transgenic mice were crossed to obtain F1 generation mice, and the F1 generation mice were self-bred and identified, and the genotype was SHP-2 flox / flox : CD4-Cre+ is a conditional knockout mouse, that is, the spinal arthritis model mouse in the present invention, specifically obtained by the following steps:

[0036] by SHP-2 flox / flox 小鼠(loxp插入位置为四号外显子即第111–176位氨基酸(rwfhghlsgkeaeklltekgkhgsflvresqshpgdfvlsvrtgddkgesndgkskvthv mircqe,见SEQ NO.1)两端,DNA序列为:aggtggttccatggtcacttgtctggaaaagaagcagagaagctgctgacggagaagggcaagcatggcagcttcctcgttcgagagagccagagccaccccggagacttcgttctctccgtgcgcactggtgacgacaaaggggagagcaacgacggcaagtccaaagtgacccacgtcatgatccgctgtcaggag,见SEQ NO.2)和CD4-Cre(CD4启动 The Cre sequence was inserted downstream of the child) and obtained by cross ...

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Abstract

The invention relates to the field of biotechnology, in particular to an establishing method of a spondyloarthritis mouse model based on the conditional knockout technology. The conditional knockout technology is adopted for specifically knocking out Ptpn11 genes (encoded SHP-2 protein) on mouse T lymphocyte, and the spondyloarthritis mouse pathological model is established. The bamboo spine pathological features spontaneously forming spondyloarthritis of a mouse are conditionally knocked out, and the model is an ideal animal model for studying the pathogenesis of spondyloarthritis and screening spondyloarthritis relieving candidate medicine.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for establishing a mouse model of spinal arthritis based on conditional knockout technology and its use. Background technique [0002] Spondyloarthritis, formerly known as spondyloarthropathies, is a group of chronic inflammatory rheumatic diseases with specific pathophysiological, clinical, radiological and genetic features, inflammatory low back pain with or without The presence of peripheral arthritis, together with certain characteristic extra-articular manifestations, is a characteristic symptom and sign of this type of disease. Diseases in this category include: ankylosing spondylitis, psoriatic arthritis, reactive arthritis, arthritis of inflammatory bowel disease, Differentiation of spondyloarthritis and juvenile chronic arthritis. [0003] The inflammatory process in spondyloarthritis occurs at the point where ligaments attach to the bone, unlike rheumatoid arthri...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/00A01K67/027
CPCA01K67/0278A61K49/0008
Inventor 孙洋徐强郭文洁吴兴新邵粉丽
Owner NANJING UNIV