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Nucleic acid lysate separation method and device based on multi-well plate

A nucleic acid lysate and separation method technology, applied in the field of nucleic acid lysate separation based on a multi-well plate, can solve problems such as displacement error and does not involve sample pretreatment steps, and achieve the effect of improving work efficiency

Active Publication Date: 2019-01-08
原态微生物检测技术(湖州)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] None of the automated microbial molecular detection technologies in the prior art involve sample pretreatment steps, that is, sample pretreatment must be done manually
Trace it to its cause, for the samples that need to be tested in large quantities, after the sample is injected into the porous plate, the porous plate oscillator in the prior art is not suitable for cooperating with other precision instruments, because the porous plate oscillator often has various defects after shaking. displacement error

Method used

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  • Nucleic acid lysate separation method and device based on multi-well plate
  • Nucleic acid lysate separation method and device based on multi-well plate
  • Nucleic acid lysate separation method and device based on multi-well plate

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Embodiment 1

[0029] In the present application, the nucleic acid lysate separation method based on a multi-well plate includes the following steps:

[0030] (1) Take the filter porous plate and the receiving porous plate, and the reaction hole of the filter porous plate is provided with a filter membrane and a passage connecting the inner and outer spaces of the reaction hole;

[0031] (2) Put an enclosure structure on the jacket of the porous plate;

[0032] (3) Cover the porous filter plate on the top of the enclosure structure so that the enclosure structure forms a closed space, and the reaction holes of the filter porous plate correspond to the reaction holes of the porous plate up and down;

[0033] (4) Inject the nucleic acid lysate into the reaction well of the filter porous plate, and this step can also be carried out before step (3);

[0034] (5) Negative pressure is formed in the closed space formed by the enclosure structure so that the nucleic acid lysate in the reaction well...

Embodiment 2

[0039] The difference between the method described in this embodiment and that of Embodiment 1 is that in step (5), the way to form negative pressure in the confined space formed by the enclosure structure is: place the receiving porous plate on the base, and the base It is connected with the negative pressure device; when the perforated plate is installed with a cover structure and a filter perforated plate, a closed space is formed between the filter perforated plate, the base and the containment structure.

[0040] Such as Figure 3-4 Shown is an apparatus corresponding to this method. The device includes a base 6, a filtering porous plate 1, a receiving porous plate 2, an enclosure structure 3, a vacuum pump 4, and a mechanical arm 5 controlled by a computer. The reaction hole of the filter porous plate 1 is provided with a filter membrane and a channel 7 connecting the inner and outer spaces of the reaction hole, the receiving porous plate 2 is located inside the enclosu...

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Abstract

The invention belongs to the technical field of determination or inspection of microorganisms, and specially relates to a method and device for separating a nucleic acid lysate based on porous plates. The methodfor separating the nucleic acid lysate based on theporous plates comprises the following steps: (1) taking a filtering porous plate and an adapting porous plate, and arranging a filter membrane and a channel for communicating inner and outer spaces of each reaction pore in reaction pores of the filtering porous plate; (2) sleeving an envelope structure outside the adapting porous plate; (3) covering the filtering porous plate on the top of the envelope structure so that the envelope structure forms a closed space; (4) injecting a nucleic acid lysate into the reaction pores of the filtering porous plate; (5) forming a negative pressure in the closed space formed by the envelope structure so that the nucleic acid lysate in the reaction pores of the filtering porous plate flows into the adapting porous plate through filter membranes below. The porous plate is used as a carrier, so that the nucleic acid lysatein the reaction pores of the filtering porous plate is automatically separated without excessive vibration.

Description

technical field [0001] The invention belongs to the technical field of microorganism determination or inspection methods, and in particular relates to a nucleic acid lysate separation method and device based on a porous plate. Background technique [0002] Before performing microbial molecular detection on a specific sample, the sample needs to be pretreated. Taking solid food as an example, the purpose of sample processing is to lyse microbial cells and enrich microorganisms. The nucleic acid lysate obtained after microbial cell lysis needs to be separated by solid-liquid separation. In the prior art, a centrifuge is generally used for separation. As stipulated in the industry standard of entry-exit inspection and quarantine of the People's Republic of China (SN / T 1870-2007): the culture medium needs to be centrifuged in a centrifuge at 8000r / min for 5min. [0003] None of the automated microbial molecular detection technologies in the prior art involves a sample pretrea...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/36C12M1/12C12M1/00C12N15/10
CPCC12M47/06C12N15/1017
Inventor 吴酬飞
Owner 原态微生物检测技术(湖州)有限公司