Exopolysaccharide-producing pediococcus pentosaceus and application thereof

A technology of Pediococcus pentosaceae and exopolysaccharide, applied in the field of microorganisms, can solve the problem of low yield, achieve strong adhesion, improve antioxidant capacity, and strong tolerance

Active Publication Date: 2016-12-14
SHAANXI SCI TECH UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the exopolysaccharide production

Method used

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  • Exopolysaccharide-producing pediococcus pentosaceus and application thereof
  • Exopolysaccharide-producing pediococcus pentosaceus and application thereof
  • Exopolysaccharide-producing pediococcus pentosaceus and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Screening and Identification of Pediococcus Pentosaceae

[0024] A total of 29 samples of pickle juice were collected in southern Shaanxi, and the strains were screened and isolated. Specific steps are as follows:

[0025] Sample pretreatment: Take 5ml of each sample with a sterilized pipette gun and put them into Erlenmeyer flasks (containing glass beads) filled with 45ml of 0.1% sterile water, shake for 1min, then let stand for 20min, and set aside.

[0026] The pretreated sample is equivalent to the original sample diluted 10 times, and the sample is successively diluted in the same way. Take 0.1mL diluted sample for each dilution, spread it on the MRS solid medium, and keep it at 37°C at a constant temperature. Cultivate for 48 hours, and use a sterile toothpick to pick a single colony that is viscous and has obvious stringiness. Stretch and isolate on MRS solid medium to obtain pure single colonies, and carry out morphological identification, including ...

Embodiment 2

[0030] The influence of embodiment 2pH on the growth of Pediococcus pentosaceae P36 bacterial strain

[0031] Regulate the pH value of MRS liquid medium with HCl (1mol / L) to be 2.0, 2.5, 3.0, 4.0, 5.0, 6.0, 6.5 and 7.0 respectively, get the Pediococcus pentosaceae P36 in the logarithmic growth phase, and use 2% inoculum size Inoculate, mix well and culture at 37°C for 18 hours, measure the absorbance value OD of the bacterial solution at 600nm wavelength 600 . See the growth of Pediococcus pentosaceae P36 in different pH image 3 , the results show that the strain can still grow in the acidic environment of pH2, indicating that it has better acid resistance.

Embodiment 3

[0032] Example 3 Tolerance of Pediococcus pentosaceae P36 to simulated gastrointestinal fluid

[0033] The artificial simulated gastrointestinal juice needs to be freshly prepared. PBS (pH 3.0) was used to configure pepsin at a concentration of 3 g / L, and filtered through a 0.22 μm membrane to prepare the desired simulated gastric juice. PBS (pH 8.0) was used to prepare trypsin at a concentration of 1 g / L, and filtered through a 0.22 μm filter membrane to prepare simulated intestinal fluid.

[0034] Pediococcus pentosaceae P36 was resuspended in normal saline, and its density was adjusted to 1×10 in simulated gastric juice (pH 3.0). 9 CFU / mL, mix thoroughly, and detect the number of viable bacteria after incubating at 37°C for 1, 2 and 3 hours. After 3 hours, take 1ml of the culture solution in the simulated gastric juice (pH 3.0) and add it to 9ml of the simulated intestinal juice (pH 8.0), mix thoroughly, and incubate at 37°C for 2, 4 and 8 hours to detect the number of vi...

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Abstract

The invention discloses exopolysaccharide-producing pediococcus pentosaceus and application thereof, and belongs to the field of microbes. The pediococcus pentosaceus provided by the invention has been preserved in CGMCC (China General Microbiological Culture Collection Center) from December 11, 2015, and a preservation number is CGMCC No:11857. A strain has the advantages that high yield of the exopolysaccharide can be realized; the exopolysaccharide yield is 153.60 mg/L. The strain has high resistant capability on NaCl, cholate and gastrointestinal fluid, and has high adhesion capability on Caco-2 cells; in addition, the oxidation resistance capability on HepG2 cells in an oxidation stress state is improved; the pediococcus pentosaceus can be used as probiotics or oxidation resistance products to be used in the fields of food, medicine, cosmetics and the like.

Description

technical field [0001] The invention relates to a strain of Pediococcus pentosaceae producing exopolysaccharide and application thereof, belonging to the field of microorganisms. Background technique [0002] Polysaccharide is the most abundant biopolymer in nature. It has many functions and is the most commonly used food additive in the food industry. It has good biological activity and can regulate the immune function of the body. It is an ideal immunomodulator . At present, a variety of polysaccharides are used in clinical trials, mainly for the research of anti-aging, anti-virus, anti-tumor and other drugs. With the in-depth research on the synthesis, preparation, structure, pharmacology and clinical science of polysaccharides, the development of polysaccharide drugs and health products has become one of the current research hotspots of scientists. Polysaccharide products will also have very broad prospects. [0003] Probiotics are a kind of active microorganisms that...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P19/04A23C9/12A23L2/38A23L33/135C12R1/01
CPCA23C9/1203A23L2/382C12P19/04C12R2001/01C12N1/205
Inventor 陈佩兰新哲党辉季晓辉裴金金金文刚
Owner SHAANXI SCI TECH UNIV
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