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Fluorescence enhancement type encoding beads and preparing method thereof

A technology of encoding microspheres and fluorescence enhancement, which is applied in the direction of microsphere preparation, microcapsule preparation, fluorescence/phosphorescence, etc., can solve the problems of reaction speed limitation, repeatability and reliability limitations, and achieve simple decoding and detection The effect of high sensitivity

Active Publication Date: 2016-12-14
SOUTHEAST UNIV
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Problems solved by technology

Although planar chip technologies are widely used in high-throughput analysis, t

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  • Fluorescence enhancement type encoding beads and preparing method thereof
  • Fluorescence enhancement type encoding beads and preparing method thereof

Examples

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Embodiment 1

[0039] Example 1 Preparation of three different encoded carrier microspheres:

[0040] 1. The ferric oxide particles with an average particle size of 7nm were added to ETPTA in proportions of 10%, 20%, and 30% by mass fraction, and dispersed uniformly, and configured into ETPTA solutions with three different magnetic nanoparticle contents.

[0041] 2. Disperse the monodisperse silica particles with a particle size of 200nm into the PEGDA precursor solution uniformly, and adjust the concentration of the silica particles to be 50%;

[0042]3. Methyl silicone oil was injected into the inlet A of the microsphere generating device through a syringe pump, ETPTA solution with a magnetic nanoparticle content of 10% was injected into the inlet B, and the prepolymer solution of silica particles was injected into the inlet C to pass the generating device. The outlet of the capsule is dispersed into spherical capsules in methyl silicone oil, and the size of the core and shell of the capsu...

Embodiment 2 3

[0046] Embodiment 2 Preparation of carrier microspheres with three different photonic crystal forbidden bands:

[0047] 1. The ferric oxide particles with an average particle size of 7nm were added to the ETPTA in a proportion of 10% by mass fraction, and dispersed uniformly, and configured into ETPTA solutions with three different contents of magnetic nanoparticles.

[0048] 2. Disperse the monodisperse silica particles with particle diameters of 160 nm, 200 nm and 240 nm into the PEGDA precursor solution respectively, and adjust the concentration of the silica particles to be 50% on average;

[0049] 3. inject methyl silicone oil into the inlet A of the microsphere generating device through a syringe pump, inject the ETPTA solution with a magnetic nanoparticle content of 10% into the inlet B, and inject the prepolymer solution with a particle size of 160nm silicon dioxide particles into the inlet C, The outlet of the generating device is dispersed into spherical capsules in ...

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Abstract

The invention discloses fluorescence enhancement type encoding beads and a preparing method thereof. The outer sides of magnetic encoded carrier beads are coated with porous photonic crystal shell structures, the forbidden band of photonic crystal is made to be matched with the excitation wavelength or emission wavelength of detection fluorescence, and then fluorescence detection signals are intensified. The preparing method comprises the steps of evenly dispersing magnetic nanoparticles into a polymer solution or a prepolymer of a polymer; evenly dispersing monodisperse silica particles in one or more solutions in the prepolymer; injecting 3-10% polyvinyl alcohol solution or methyl silicone oil or n-hexadecane into a bead generation device through an injection pump; injecting the polymer solution containing the magnetic nanoparticles and the prepolymer solution containing the silicon dioxide particles into the generation device, obtaining spherical capsules at an outlet, conducting drying and curing, treating cured capsules with 2-4% HF aqueous solution, removing silicon dioxide particles from a shell, and finally obtaining magnetic content encoded polymer carrier beads with outer sides coated with pored photonic crystal shells.

Description

technical field [0001] The invention relates to a magnetically encoded carrier microsphere which utilizes a porous photonic crystal shell structure covered on the outside of the magnetically encoded carrier microsphere and utilizes the photonic crystal to enhance the fluorescence signal and a preparation method thereof. Background technique [0002] With the rapid development of biomedicine, high-throughput biological analysis is required in the fields of disease detection and drug discovery. Although planar chip technologies are widely used in high-throughput analysis, they have certain limitations in response speed, and certain limitations in reproducibility and reliability. Suspension arrays are another alternative strategy, in which the probe molecule carriers can be freely distributed into the reaction system without being restricted by spatial locations. For suspended arrays, there are two key issues. One is the need to encode the flow carrier. In recent years, vari...

Claims

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Application Information

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IPC IPC(8): G01N21/64B01J13/14B01J13/02
CPCB01J13/02B01J13/14G01N21/6428G01N2021/6439
Inventor 谢卓颖陈姗赵远锦顾忠泽
Owner SOUTHEAST UNIV
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