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Method for extracting bioactive ingredients in rhizoma polygoni cuspidati

A technology of bioactive ingredients and extraction methods, which is applied in the direction of sugar derivatives, sugar derivatives, sugar derivatives preparation, etc., can solve the problems of no comprehensive utilization of purification processes, etc., and achieve no environmental pollution, small usage, and equipment simple effect

Active Publication Date: 2016-12-21
HUNAN NUSTREETCARAX
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] As a traditional Chinese medicine, Polygonum cuspidatum has been well known in my country for a long time. After decades of modern medicinal and pharmacological research, especially since the research and development of Polygonum cuspidatum extract, firstly, from Polygonum cuspidatum extract with emodin as the symbolic component to the current fermentation From the extraction of resveratrol to the separation and purification of polydatin and then fermentation to prepare resveratrol, etc., but so far there is no comprehensive utilization of the purification process

Method used

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  • Method for extracting bioactive ingredients in rhizoma polygoni cuspidati

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Experimental program
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Effect test

Embodiment 1

[0049] The steps of the method for extracting the biologically active components of Polygonum cuspidatum in this embodiment are as follows:

[0050] 1. Take Polygonum cuspidatum (dry) 500KG, (HPLC detection contains emodin 0.61%, polydatin 2.47%, resveratrol 0.72%), crush through a 20-mesh sieve, and put it into a reflux extraction tank;

[0051] 2) The first reflux extraction: 4000 kg of 80% alcohol, 1% KOH to adjust the pH to 9.0, the temperature is controlled at 60 degrees, and reflux for 3 hours;

[0052] 3) The second reflux: 3000 kg of 80% alcohol, 1% KOH to adjust the pH to 9.0, the temperature is controlled at 60 degrees, reflux for 2 hours;

[0053] 4) Combine the reflux liquid and neutralize: combine the two reflux liquids, neutralize with HCl to pH 6.5-7.0, add saturated ammonium sulfate, and filter to remove the precipitate;

[0054] 5) Alcohol recovery: recover the alcohol from the filtered extract until the alcohol content is below 15% to obtain 2700 kg of recov...

Embodiment 2

[0065] The steps of the method for extracting the biologically active components of Polygonum cuspidatum in this embodiment are as follows:

[0066] 1. Take Polygonum cuspidatum (dried) 500KG, (HPLC detection contains emodin 0.63%, polydatin 2.41%, resveratrol 0.75%), pulverize through a 20-mesh sieve, and put it into a reflux extraction tank;

[0067] 2) The first reflux extraction: 4000 kg of 80% alcohol 1% KOH to adjust the pH to 9.0, and the temperature was controlled at 60 degrees for 3 hours;

[0068] 3) Second reflux: 3000 kg of 80% alcohol, 1% KOH to adjust pH to 9.0, temperature controlled at 60 degrees, reflux for 2 hours;

[0069] 4) Combine the reflux liquid and neutralize: combine the two reflux liquids, neutralize with HCl to pH 6.5-7.0, add saturated ammonium sulfate, and filter to remove the precipitate;

[0070] 5) Alcohol recovery: recover alcohol from the filtered extract until the alcohol content is below 15% to obtain 2810 kg of recovery solution;

[007...

Embodiment 3

[0081] The steps of the method for extracting the biologically active components of Polygonum cuspidatum in this embodiment are as follows:

[0082] 1. Take Polygonum cuspidatum (dry) 500KG, (HPLC detection contains emodin 0.60%, polydatin 2.44%, resveratrol 0.69%), crush through a 20-mesh sieve, and put it into a reflux extraction tank;

[0083] 2) The first reflux extraction: 4000 kilograms of 80% alcohol 1% KOH to adjust the pH to 9.0, and the temperature is controlled at 60 degrees to reflux for 3 hours;

[0084]3) The second reflux: 3000 kg of 80% alcohol, 1% KOH to adjust the pH to 9.0, the temperature is controlled at 60 degrees, reflux for 2 hours;

[0085] 4) Combine the reflux liquid and neutralize: combine the two reflux liquids, neutralize with HCl to pH 6.5-7.0, add saturated ammonium sulfate, and filter to remove the precipitate;

[0086] 5) Alcohol recovery: recover the alcohol from the filtered extract until the alcohol content is below 15% to obtain 2600 kg o...

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Abstract

The invention discloses a method for extracting bioactive ingredients in rhizoma polygoni cuspidati. The method includes the following steps that rhizoma polygoni cuspidati is taken, ground, screened with a 18-22-mesh sieve and put into a reflux tank; 75-85% ethyl alcohol with the weight 6-10 times that of rhizoma polygoni cuspidati is added, and reflux is carried out for 2.5-3.5 h at 55-65 DEG C; saturated ammonium sulfate is added into reflux liquid till precipitate is generated, and filtering is carried out to remove precipitate; ethyl alcohol is recycled, and alcohol extraction liquid is obtained; concentration is carried out, and concentrated extract is obtained; water precipitation is carried out, and supernatant liquid is taken; concentration is carried out again, and concentrated extract is obtained; alcohol dissolution is carried out, and supernatant liquid is taken; the supernatant liquid is subjected to plate ultrafiltration with SPES, and precipitate and supernatant liquid are collected; separation and purification of rheum emodin are carried out, wherein the purity of rheum emodin is 99.8%; the supernatant liquid is adsorbed with macroporous resin; separation and purification of polydatin are carried out, wherein the purity of polydatin is 99.5% or above; separation and purification of resveratrol are carried out, wherein the purity of resveratrol is 99.2% or above. According to the method, adopted equipment is simple, the process is simple and feasible, and industrial production is convenient; an adopted solvent is free of toxins or residues and does not cause environmental pollution.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine, in particular to a method for extracting biologically active components of Polygonum cuspidatum. Background technique [0002] Polygonum cuspidatum is a traditional Chinese medicine. It tastes slightly bitter, astringent, and slightly cold in nature. It belongs to the liver, gallbladder, and lung meridians. Polygonum cuspidatum contains a large number of biologically active components through modern pharmacological and pharmacological studies, mainly stilbenotriphenols (polydatin and resveratrol), anthraquinones (emodin, etc.). [0003] Resveratrol is an active polyphenol compound, which has strong anti-oxidation, anti-aging, free radical scavenging effects, and has a strong inhibitory effect on canceration and cell tissue variation. It is another green anti-cancer drug after paclitaxel. Anticancer active substance. Moreover, resveratrol has the effect of preventing arterio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C46/10C07C50/34C07H1/08C07H15/203C07C39/21C07C37/68C07C37/70C07C37/82
CPCC07C37/685C07C37/70C07C37/82C07C39/21C07C46/10C07C50/34C07H1/08C07H15/203
Inventor 张宝堂谭家忠
Owner HUNAN NUSTREETCARAX
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