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A method for extracting anthocyanins from Lycium barbarum

A technology of medlar and anthocyanin is applied in chemical instruments and methods, organic chemistry, azo dyes and other directions, can solve the problems of low yield and low purity, and achieves the effects of simple process steps and good industrial application prospects.

Active Publication Date: 2019-03-05
中科果悦湖州生物科技开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, it is reported at home and abroad that the separation of anthocyanins from Lycium barbarum is mainly based on the fruit of Lycium barbarum, and solvent extraction is used, supplemented by modern technologies such as ultrasound, microwave, and enzymes. However, all of them are crude jacquard anthocyanins. And the yield is low. For example, Li Jin et al., "Research on the Extraction and Refining Technology of Black Fruit Lycium Barbarum Pigment" Natural Product Research and Development, No. 18, 2006, pages 650-654 disclose a kind of extraction and refining of black fruit Lycium barbarum pigment technology, but its yield is only 9.07%, and the color value of the obtained Lycium barbarum anthocyanins is 36.7, and the purity is not high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: Efficient preparation method of high-purity Lycium barbarum anthocyanins of the present invention

[0027] 1. Preparation method

[0028] (1) Drying the black fruit wolfberry, pulverizing, and passing through a 60-mesh sieve to obtain black fruit wolfberry powder.

[0029] (2) Mix the black fruit wolfberry powder and the extraction solvent 60% ethanol solution evenly, the extraction temperature is 40°C, the solid-liquid ratio is 1:10, the extraction time is 40min, extract 4 times, and centrifuge at 2000rpm to obtain the black fruit wolfberry extract .

[0030] (3) The extract is processed through a microfiltration membrane-ultrafiltration membrane-nanofiltration membrane system. Among them, the pore size of the microfiltration membrane is 0.01μm, the temperature is 20°C, the pressure is 2.0bar, and the flow rate is 30kg / h, and impurities such as suspended solids, bacteria and large molecular weight colloids are removed to obtain the permeate of the microfil...

Embodiment 2

[0041] Example 2: Efficient preparation method of high-purity Lycium barbarum anthocyanins of the present invention

[0042] 1. Preparation method

[0043] (1) Drying the black fruit wolfberry, pulverizing, and passing through a 60-mesh sieve to obtain black fruit wolfberry powder.

[0044] (2) Mix the black fruit wolfberry powder and the extraction solvent 70% ethanol solution evenly, the extraction temperature is 50°C, the solid-liquid ratio is 1:30, the extraction time is 60min, extract 3 times, and centrifuge at 2000rpm to obtain the black fruit wolfberry extract .

[0045] (3) The extract is processed through a microfiltration membrane-ultrafiltration membrane-nanofiltration membrane system. Among them, the microfiltration membrane has a pore size of 10 μm, a temperature of 20°C, a pressure of 2.0 bar, and a flow rate of 30 kg / h to remove impurities such as suspended solids, bacteria, and large molecular weight colloids to obtain the permeate of the microfiltration memb...

Embodiment 3

[0056] Example 3: Efficient preparation method of high-purity Lycium barbarum anthocyanins of the present invention

[0057] 1. Preparation method

[0058] (1) Drying the black fruit wolfberry, pulverizing, and passing through a 60-mesh sieve to obtain black fruit wolfberry powder.

[0059] (2) Mix the black fruit wolfberry powder and the extraction solvent 80% ethanol solution evenly, the extraction temperature is 60°C, the solid-liquid ratio is 1:20, the extraction time is 50min, extract twice, and centrifuge at 2000rpm to obtain the black fruit wolfberry extract .

[0060] (3) The extract is processed through a microfiltration membrane-ultrafiltration membrane-nanofiltration membrane system. Among them, the microfiltration membrane has a pore size of 1 μm, a temperature of 20°C, a pressure of 2.0 bar, and a flow rate of 30 kg / h to remove impurities such as suspended solids, bacteria, and large molecular weight colloids to obtain the microfiltration membrane permeate; the ...

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Abstract

The invention discloses a preparation method of lycium ruthenicum anthocyanin. The method comprises the following steps that 1, lycium ruthenicum is dried and crushed to obtain lycium ruthenicum powder; 2, the lycium ruthenicum powder and a 60%-80% ethanol solution are mixed to be uniform, and extracting is conducted; 3, an extracting solution is treated sequentially through a micro-filtration membrane, an ultra-filtration membrane and a nano-filtration membrane; 4, ingredients retained by the nano-filtration membrane are dissolved and fed into a non-polar macroporous resin column; 5, lycium ruthenicum anthocyanin eluant is subjected to vacuum concentration and dried, and the lycium ruthenicum anthocyanin is obtained. According to the method, the lycium ruthenicum anthocyanin can be effectively extracted, the extraction rate is high, the process steps are simple, the cost is low, and the lycium ruthenicum anthocyanin prepared through the method is very high in purity and good in effect.

Description

technical field [0001] The invention relates to a method for extracting high-purity Lycium barbarum anthocyanins. Background technique [0002] Black fruit wolfberry (Lycium ruthenicum Murr.) is the mature fruit of Lycium ruthenicum Murr., which is used for both medicine and food. It is also called "Pangma" in Tibetan medicine. "Jingzhu Materia Medica" records that the fruit of Pangma is sweet, can clear away heart-heat, and treat heart disease and gynecological diseases. It is recorded as "Pang Na Jie Wu" in "Chinese Tibetan Medicine". Rang Jun Duoji said: "Goji berry cures heart disease." "Illustrated Book" said: "Goji berries are thin leaves, shrubs, purple-red fruit, sweet, and effective in clearing away heat." The fruit has the functions of name and purpose, strengthening the kidney, nourishing the liver, and dredging the meridian. Its fruit and root bark can treat urethral calculus, ringworm, scabies, and bleeding gums. Black fruit wolfberry is rich in nutrients, su...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H17/065C07H1/08C09B61/00
CPCC07H1/08C07H17/065C09B61/00
Inventor 索有瑞王树林刘博
Owner 中科果悦湖州生物科技开发有限公司