Compound microecological preparation for livestock and poultry excrement treatment and preparation method and application thereof
A technology of livestock and poultry excrement and compound microecology, which is applied in the field of microecological preparations, can solve problems such as secondary pollution, large investment in production equipment, and generation of ammonia odor, and achieve reduced energy consumption, fast processing time, and reduced processing time. cost effect
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Embodiment 1
[0027] 1. Test materials and methods
[0028] 1.1 Test material
[0029] 1.1.1 Strain selection
[0030] Use Lactobacillus casei (CGMCC1.62) Lactobacillus casei , Candida utilis (CGMCC2.1004) Candida utilis , Enterococcus faecalis (CGMCC1.101) Enterococcus faecalis , Bacillus subtilis (CGMCC1.504) Bacillus subtilis , and the above strains were purchased from China General Microorganism Culture Collection and Management Center.
[0031] 1.1.2 Instruments and equipment
[0032] Electric constant temperature water-proof incubator (Shanghai Yuejin Medical Machinery Factory);
[0033] Vertical high-pressure steam sterilizer (Shanghai Shen'an Medical Instrument Co., Ltd.);
[0034] BCM-1000 biological purification workbench (Suzhou Purification Equipment Co., Ltd.);
[0035] Double-layer steam bath oscillator (Jiangsu Jintan Jerry Electric Co., Ltd.);
[0036] PHS-2C acidity meter (Tianjin Saidelis Experimental Analysis Instrument Factory);
[0037] 79-1 magnetic stirr...
Embodiment 2
[0100] 2.1 Compound probiotics
[0101] According to the single factor test results in Example 1, the bacterial liquid of Lactobacillus casei, Enterococcus faecalis, Candida utilis and Bacillus subtilis fermented and cultivated livestock and poultry excrement, all of which can reduce Escherichia coli therein to varying degrees. It can reduce the number and indole content, eliminate the odor of livestock and poultry excrement, and play an important role in the harmless treatment of livestock and poultry excrement.
[0102] In this example, based on 100 g of livestock and poultry excrement, the following volume ranges of bacterial liquids were used: Lactobacillus casei 0.03-0.10 mL, Enterococcus faecalis 0.03-0.10 mL, Candida utilis 0.05-0.50 mL, Bacillus subtilis Bacillus 0.03~0.10 mL, take different volume values to combine (see Table 5 below), mix well to get multiple combinations of compound probiotics, and use the compound probiotics for fermentation and culture tests of ...
Embodiment 3
[0125] Example 3: Effects of compound probiotics on Escherichia coli and odor from livestock and poultry excrement
[0126] The compound microecological preparation with the optimal proportion obtained in Example 2 is carried out the fermentation culture test of livestock and poultry excrement, counting 100g of livestock and poultry excrement, the bacterial liquid of following volume: Lactobacillus casei 0.06 mL, Enterococcus faecalis 0.07 mL, 0.15 mL of Candida utilis, and 0.07 mL of Bacillus subtilis were mixed evenly to obtain a compound microecological preparation, and the compound microecological preparation was used as a fermentation test of livestock and poultry excrement. The test method of fermentation culture was the same as that in 2.1. The method in the paper is to use a 250 mL Erlenmeyer flask with a sampling volume of 200 g, inoculate the obtained compound microecological preparation according to the ratio, place it in a 37°C incubator, take a sample after 120 h, ...
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