Compound microecological preparation for livestock and poultry excrement treatment and preparation method and application thereof

A technology of livestock and poultry excrement and compound microecology, which is applied in the field of microecological preparations, can solve problems such as secondary pollution, large investment in production equipment, and generation of ammonia odor, and achieve reduced energy consumption, fast processing time, and reduced processing time. cost effect

Inactive Publication Date: 2016-12-21
HENAN PUAI FEED GRP CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chemical method is a method of using chemical reagents to treat feces. The principle is that organic substances in feces react with chemical substances, such as H 2 S can be oxidized to hydrogen sulfide or sulfur or sulfur dioxide. Due to the unstable effect, the application effect of this technology is not good.
Physical methods mainly include t...

Method used

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  • Compound microecological preparation for livestock and poultry excrement treatment and preparation method and application thereof
  • Compound microecological preparation for livestock and poultry excrement treatment and preparation method and application thereof
  • Compound microecological preparation for livestock and poultry excrement treatment and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1. Test materials and methods

[0028] 1.1 Test material

[0029] 1.1.1 Strain selection

[0030] Use Lactobacillus casei (CGMCC1.62) Lactobacillus casei , Candida utilis (CGMCC2.1004) Candida utilis , Enterococcus faecalis (CGMCC1.101) Enterococcus faecalis , Bacillus subtilis (CGMCC1.504) Bacillus subtilis , and the above strains were purchased from China General Microorganism Culture Collection and Management Center.

[0031] 1.1.2 Instruments and equipment

[0032] Electric constant temperature water-proof incubator (Shanghai Yuejin Medical Machinery Factory);

[0033] Vertical high-pressure steam sterilizer (Shanghai Shen'an Medical Instrument Co., Ltd.);

[0034] BCM-1000 biological purification workbench (Suzhou Purification Equipment Co., Ltd.);

[0035] Double-layer steam bath oscillator (Jiangsu Jintan Jerry Electric Co., Ltd.);

[0036] PHS-2C acidity meter (Tianjin Saidelis Experimental Analysis Instrument Factory);

[0037] 79-1 magnetic stirr...

Embodiment 2

[0100] 2.1 Compound probiotics

[0101] According to the single factor test results in Example 1, the bacterial liquid of Lactobacillus casei, Enterococcus faecalis, Candida utilis and Bacillus subtilis fermented and cultivated livestock and poultry excrement, all of which can reduce Escherichia coli therein to varying degrees. It can reduce the number and indole content, eliminate the odor of livestock and poultry excrement, and play an important role in the harmless treatment of livestock and poultry excrement.

[0102] In this example, based on 100 g of livestock and poultry excrement, the following volume ranges of bacterial liquids were used: Lactobacillus casei 0.03-0.10 mL, Enterococcus faecalis 0.03-0.10 mL, Candida utilis 0.05-0.50 mL, Bacillus subtilis Bacillus 0.03~0.10 mL, take different volume values ​​to combine (see Table 5 below), mix well to get multiple combinations of compound probiotics, and use the compound probiotics for fermentation and culture tests of ...

Embodiment 3

[0125] Example 3: Effects of compound probiotics on Escherichia coli and odor from livestock and poultry excrement

[0126] The compound microecological preparation with the optimal proportion obtained in Example 2 is carried out the fermentation culture test of livestock and poultry excrement, counting 100g of livestock and poultry excrement, the bacterial liquid of following volume: Lactobacillus casei 0.06 mL, Enterococcus faecalis 0.07 mL, 0.15 mL of Candida utilis, and 0.07 mL of Bacillus subtilis were mixed evenly to obtain a compound microecological preparation, and the compound microecological preparation was used as a fermentation test of livestock and poultry excrement. The test method of fermentation culture was the same as that in 2.1. The method in the paper is to use a 250 mL Erlenmeyer flask with a sampling volume of 200 g, inoculate the obtained compound microecological preparation according to the ratio, place it in a 37°C incubator, take a sample after 120 h, ...

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Abstract

The invention aims at providing a compound microecological preparation for livestock and poultry excrement treatment and a preparation method and application thereof. For 100 g of livestock and poultry excrement, the compound microecological preparation needed to be added is prepared from, by volume, lactobacillus casei 0.03-0.10 Ml, enterococcus faecalis 0.03-0.10 mL, candida mycoderma bacteria 0.05-0.50 mL and bacillus subtilis 0.03-0.10 mL, wherein effective living bacterium number of bacterium liquid of the lactobacillus casei, the enterococcus faecalis, the candida mycoderma bacteria and the bacillus subtilis is 1.0 * 109 cfu/mL. The compound microecological preparation is utilized for fermentation of livestock and poultry excrement, the pH and the enterococcus faecalis number of the preparation can be reduced (P> 0.05), the content of indole is reduced by 58% (P< 0.05), and the compound microecological preparation has the great significance on fecal odor elimination.

Description

technical field [0001] The invention belongs to the technical field of probiotics, and in particular relates to a composite probiotic for treating livestock and poultry excrement, a preparation method and application thereof. Background technique [0002] According to reports, the annual production of livestock and poultry manure in the country is about 1.9 billion tons, which is 2.4 times that of industrial solid waste. In addition to the discharge of sewage, the waste discharged by the aquaculture industry in my country is as high as more than 6 billion tons every year; livestock and poultry waste Pollution has become one of the main causes of pollution. While the amount of livestock and poultry is kept increasing, the rural construction land reduces the area of ​​farmland that can effectively carry livestock and poultry waste. With the continuous increase of intensive farming models, the output of livestock and poultry waste exceeds the load that local farmland can carry, ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16C05F3/00C05F17/00C12R1/245C12R1/46C12R1/72C12R1/125
CPCC05F3/00C05F17/00C12N1/16C12N1/20C05F11/08Y02A40/20Y02W30/40
Inventor 尹清强路文华常娟王潇刘超齐卢富山
Owner HENAN PUAI FEED GRP CO LTD
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