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Stable kit for diagnostically detecting aspartate aminotransferase

A technology of aspartic acid amino group and diagnostic kit, which is applied in the field of medical testing, and can solve problems such as test results being easily questioned, biochemical reagent storage, transportation and use troubles, etc.

Active Publication Date: 2016-12-21
BIOBASE BIODUSTRY (SHANDONG) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] So far, hospitals generally use automatic biochemical analyzers for testing, but the instability of biochemical reagents has caused certain troubles to the storage, transportation and use of the reagents, and the test results are also easily questioned. In view of the above problems, A stable aspartate aminotransferase detection kit of the present invention is mainly through the reorganization of a group of composite stabilizers. This method is different from the previous ones who simply added stabilizers and surfactants to prolong the use and storage of reagents. time is completely different

Method used

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  • Stable kit for diagnostically detecting aspartate aminotransferase
  • Stable kit for diagnostically detecting aspartate aminotransferase
  • Stable kit for diagnostically detecting aspartate aminotransferase

Examples

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Effect test

Embodiment 1

[0052] In the specific implementation of the present invention, the described reagent R1 is a composite stabilizer with a content of 5.5%, and its composition and content are as follows:

[0053] EDTA-Na 2 2.5g / L

[0054] Glycerol 75ml / L

[0055] Mannitol 10g / L

[0056] Latong X-405 7.5ml / L

[0057] Dextran-2000 9.5g / L

[0058] Trimethylolmethane 10g / L

[0059] Sodium azide 3g / L

[0060] In the concrete implementation of the present invention, described reagent R2 is that the composite stabilizer content is 7.5%, and its composition and content are as follows:

[0061] EDTA-Na 2 2.0g / L

[0062] Glycerol 55ml / L

[0063] Mannitol 8g / L

[0064] Latong X-405 5.5ml / L

[0065] Dextran-2000 7.5g / L

[0066] Trimethylolmethane 8.0g / L

[0067] Sodium azide 3g / L

Embodiment 2

[0069] In the specific implementation of the present invention, the described reagent R1 is a composite stabilizer with a content of 7.5%, and its composition and content are as follows:

[0070] EDTA-Na 2 2.5g / L

[0071] Glycerol 75ml / L

[0072] Mannitol 10g / L

[0073] Latong X-405 7.5ml / L

[0074] Dextran-2000 9.5g / L

[0075] Trimethylolmethane 10g / L

[0076] Sodium azide 3g / L

[0077] In the concrete implementation of the present invention, described reagent R2 is that the composite stabilizer content is 7.5%, and its composition and content are as follows:

[0078] EDTA-Na 2 2.5g / L

[0079] Glycerol 75ml / L

[0080] Mannitol 10g / L

[0081] Latong X-405 7.5ml / L

[0082] Dextran-2000 9.5g / L

[0083] Trimethylolmethane 10g / L

[0084] Sodium azide 3g / L

Embodiment 3

[0086] In the concrete implementation of the present invention, described reagent R1 is that the compound stabilizer content is 10%, and its composition and content are as follows:

[0087] EDTA-Na 2 2.5g / L

[0088] Glycerol 75ml / L

[0089] Mannitol 10g / L

[0090] Latong X-405 7.5ml / L

[0091] Dextran-2000 9.5g / L

[0092] Trimethylolmethane 10g / L

[0093] Sodium azide 3g / L

[0094] In the concrete implementation of the present invention, described reagent R2 is that the compound stabilizer content is 5.5%, and its composition and content are as follows:

[0095] EDTA-Na 2 2.5g / L

[0096] Glycerol 75ml / L

[0097] Mannitol 10g / L

[0098] Latong X-405 7.5ml / L

[0099] Dextran-2000 9.5g / L

[0100] Trimethylolmethane 10g / L

[0101] Sodium azide 3g / L

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Abstract

The invention relates to a stable kit for diagnostically detecting aspartate aminotransferase; the kit finishes detection by using a composite stabilizer instead of a previous independent stabilizer; the composite stabilizer is a novel efficient stabilizer and may have a different composition for a different biochemical reagent, but is important to protecting related enzymes in reagents; the stabilizer acts mainly in the following way: the composite stabilizer forms a spherical film under certain solute and external conditions, and the spherical film may wrap organic enzymes and release the enzymes for reaction in the case of substrate induction; a direction is provided for processing and improving instability factors of biochemical reaction that the enzymes in biochemical reagents engage in, and basis is laid for the marketing and long-term development of the kit.

Description

technical field [0001] The invention relates to the technical field of medical inspection, in particular to the problem of automatically detecting the activity of aspartate aminotransferase in human serum by using a fully automatic biochemical analyzer. Background technique [0002] The soundness of liver function is directly related to our own life safety. In recent years, due to the rapid economic development and the improvement of people's living standards, liver diseases caused by a series of adverse problems deserve special attention. Liver disease is a common disease, which is very harmful and takes the life of patients in severe cases. Therefore, it is particularly important for the early diagnosis and prevention of liver disease. Aspartate aminotransferase is widely distributed in various tissues in the body, among which Higher heart activity. Higher aspartate aminotransferase activity suggests liver cell damage, which generally occurs in fatty liver, hepatitis B, c...

Claims

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Application Information

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IPC IPC(8): C12Q1/52C12Q1/32C12Q1/26
CPCC12Q1/26C12Q1/32C12Q1/52
Inventor 李建营甘宜梧李静
Owner BIOBASE BIODUSTRY (SHANDONG) CO LTD
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