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A multi-component surface-enhanced Raman spectroscopy detection method based on composite photonic crystal microspheres

A technology of photonic crystal microspheres and surface-enhanced Raman, which is applied in the field of biomedical detection, can solve the problems of photonic crystal-encoded microspheres without Raman enhancement, the inability to realize multivariate analysis of the detection range, and insufficient stability of the signal, so as to achieve clear distinction, Stable signal and good uniformity

Active Publication Date: 2018-09-04
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the Raman-encoded microspheres and SiO 2 The photonic crystal encoded microsphere itself does not have the effect of Raman enhancement, but only plays the function of encoding
In summary, the existing SERS detection methods still have defects such as insufficient signal stability, low sensitivity, and poor reproducibility during detection, and cannot achieve multivariate analysis with a wide detection range.

Method used

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  • A multi-component surface-enhanced Raman spectroscopy detection method based on composite photonic crystal microspheres
  • A multi-component surface-enhanced Raman spectroscopy detection method based on composite photonic crystal microspheres
  • A multi-component surface-enhanced Raman spectroscopy detection method based on composite photonic crystal microspheres

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] (1) Preparation of composite photonic crystal microspheres: First, 200 μL (500 μg / mL) of streptavidin solution was added to 0.006 g of prepared silver nanoparticles-coated titanium dioxide photonic crystal microspheres at 4°C overnight, and 100 μL of 1% The bovine serum albumin was blocked at room temperature for 2 hours, and the blocked microspheres were washed with phosphate buffer solution before use; at the same time, 1 mL of the prepared three colors of green, yellow, and red water-phase CdTe quantum dots were respectively taken ( 0.8mmol / L) were mixed with biotin 200μL (1mg / mL) at room temperature, and placed in a shaker for 3h reaction; the above silver nanoparticles coated titanium dioxide photonic crystal microspheres modified with streptavidin into the three kinds of quantum dot solutions modified with biotin, shake the bed at room temperature for 3 hours, and finally wash three times with phosphate buffer solution;

[0028] (2) Immobilization of antibodies: e...

Embodiment 2

[0032] (1) Preparation of composite photonic crystal microspheres: First, 200 μL (500 μg / mL) of streptavidin solution was added to 0.007 g of prepared silver nanoparticles-coated titanium dioxide photonic crystal microspheres at 4°C overnight, and 100 μL of 1% The bovine serum albumin was blocked at room temperature for 2 hours, and the blocked microspheres were washed with phosphate buffer solution before use; at the same time, 1.4 mL of the prepared three colors of green, yellow, and red water-phase CdTe quantum dots were taken. (0.8mmol / L) were mixed with biotin 200μL (1mg / mL) at room temperature, and put into a shaker to react for 3h; the silver nanoparticles coated titanium dioxide photonic crystal microspheres modified with streptavidin were Add to the three kinds of quantum dot solutions modified with biotin, shake the bed at room temperature for 3 hours, and finally wash with phosphate buffer solution for 3 times;

[0033] (2) Immobilization of antibodies: 0.0075 g of ...

Embodiment 3

[0037] (1) Preparation of composite photonic crystal microspheres: First, 200 μL (500 μg / mL) of streptavidin solution was added to 0.008 g of prepared silver nanoparticles-coated titanium dioxide photonic crystal microspheres at 4°C overnight, and 100 μL of 1% The bovine serum albumin was blocked at room temperature for 2 hours, and the blocked microspheres were washed with phosphate buffer solution before use; at the same time, 1.6 mL of the prepared three colors of green, yellow, and red water-phase CdTe quantum dots were respectively taken. (0.8mmol / L) were mixed with biotin 200μL (1mg / mL) at room temperature, and put into a shaker to react for 3h; the silver nanoparticles coated titanium dioxide photonic crystal microspheres modified with streptavidin were Add to the three kinds of quantum dot solutions modified with biotin, shake the bed at room temperature for 3 hours, and finally wash with phosphate buffer solution for 3 times;

[0038] (2) Immobilization of antibodies:...

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Abstract

The invention discloses a multi-component surface-enhanced Raman spectroscopy detection method based on composite photonic crystal microspheres. Quantum dots are evenly modified on titanium dioxide photonic crystal microspheres coated with silver nanoparticles through an avidin-biotin system. Surface, generate coded composite photonic crystal microsphere materials, combine quantum dots with silver nanoparticles coated titanium dioxide photonic crystal microsphere substrate materials in multi-component surface-enhanced Raman spectroscopy detection, through the encoding / decoding of quantum dots Combined with Raman signals, multiplex detection of different biomolecules is realized. The invention uses silver nanoparticles coated titanium dioxide photonic crystal microspheres as the encoding carrier, which has the advantages of large specific surface area, high biocompatibility, simple and quick operation process, etc. when performing biological detection. Combined, they can be effectively applied to multiple detection in biological detection.

Description

technical field [0001] The invention belongs to the technical field of biomedical detection, and in particular relates to a multi-component surface-enhanced Raman spectrum detection method based on composite photonic crystal microspheres. Background technique [0002] Early analysis and detection of tumor markers is of great significance in clinical diagnosis. Due to the complexity of carcinogenesis, many biomarkers are not specific to a cancer. Therefore, it is necessary to develop methods for simultaneous determination of multiple tumor markers. Compared with single-component detection, multiple detection technology simplifies the analysis process, shortens the analysis time, reduces the analysis cost, and makes it possible to obtain a huge amount of biomolecular information, which has become a research hotspot in recent years. [0003] Surface-enhanced Raman scattering (SERS) has been widely used in biological immunology and other fields as an analysis and detection meth...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 李娟童晶晶李新卉李宛玲刁国旺
Owner YANGZHOU UNIV
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