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An organic acid-based method for discriminating the quality of fresh tobacco leaf samples in tobacco metabolomics

A technology of metabolomics and fresh tobacco leaves, applied in the field of analytical chemistry, can solve problems that have never been raised before, and achieve the effect of less sample consumption, simple and fast operation, and accurate and reliable results

Active Publication Date: 2018-11-30
ZHENGZHOU TOBACCO RES INST OF CNTC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In view of the important role of organic acids in tobacco quality, reports on the extraction and detection of organic acids in tobacco have emerged in recent years, but no one has proposed the use of organic acid content or content ratio in fresh tobacco leaves for metabolomics fresh tobacco leaves Judgment of sample quality

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The content of organic acids in fresh tobacco leaf samples was determined by GC-MS method, and the ratio of heptadecanoic acid to butyric acid in browned samples was found to be significantly increased.

[0018] The samples to be tested included 111 fresh tobacco leaf samples from 6 varieties and 19 origins in Fujian, Guizhou, Hunan, Henan, Yunnan and other provinces (5-6 biological replicates for each point). These include 87 qualified samples (samples were strictly controlled at -80°C during collection, transportation and pretreatment), and 24 browned samples (completely browned due to insufficient liquid nitrogen during storage and transportation).

[0019] The sample analysis steps are as follows:

[0020] The specific methods are as follows: 1) Fresh tobacco leaf samples picked from the field were stored and transported in a liquid nitrogen tank at -196°C, frozen and ground in liquid nitrogen, freeze-dried at a low temperature, and stored in a refrigerator at -80°C...

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Abstract

The invention provides a method of determining quality of a fresh tobacco leaf sample in organic acid-based tobacco metabonomics. According to the method, a ratio of daturic acid to butyric acid in the fresh tobacco leaf sample is taken as a target to determine the quality of the fresh tobacco leaf sample in the metabonomics, and a basis is provided for the selection of a qualified sample from the metabonomics. The method provided by the invention is accurate, reliable, simple and feasible, and can provide a reference for tobacco metabolic characteristic analysis and relative tobacco metabonomic studies.

Description

technical field [0001] The invention relates to the field of analytical chemistry, in particular to a method for judging whether the quality of fresh tobacco leaf samples used in tobacco metabolomics research meets the needs of metabolomics research. Background technique [0002] Plant metabolomics is a technique to study plants by examining the changes of metabolites or their changes over time after plants are stimulated or disturbed. In the process of metabolomics profiling analysis, the collection of typical samples is the first and extremely important step. Obtaining qualified samples to truly and objectively reflect the metabolic state of the sample is the basis and premise of metabolomics analysis. The collection of fresh tobacco leaf samples needs to be quenched at low temperature to stop its physiological activities to ensure its metabolic state at the time of sampling. The standard practice is to pick plant samples, wrap them in tin foil, and place them in liquid ni...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/062
Inventor 陈霞周会娜刘萍萍金立锋徐国云郑庆霞翟妞陈千思李锋武明珠
Owner ZHENGZHOU TOBACCO RES INST OF CNTC