Detecting chip used for dynamically detecting interactive action of biomolecules and preparation method thereof

A detection chip and dynamic detection technology, which is applied in the direction of measuring devices, material analysis, and material analysis through optical means, can solve the problems of fluidity detection signal interference, inconvenient chip installation, and increased operation volume, so as to increase added value, Mild operating conditions to ensure consistent results

Active Publication Date: 2017-02-01
苏州奥普特克自动化科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology describes an improved way to detect molecules that interact with each other through their own chemical bondings called binding partners or ligands. These particles have specific properties such as shape, color, charge, etc., making it easier than regular liquids like water (which could be difficult). They also work well at low temperatures without causing damage from freezing. Overall this innovative technical means improve the efficiency and accuracy of identifying these tiny structures within complex samples while reducing environmental concerns associated therewith.

Problems solved by technology

Technological Problem: Biomedial molecule reactions are complicated processes involving multiple variables such as temperature or pressure levels for example. Current methods require expensive equipment and long periods of analysis before accurate data results may be obtained due to poorly controlled environmental conditions. Additionally, current techniques have limitations where they cannot detect small changes between different temperatures or pressures without affecting their accuracy.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The preparation method of the detection chip for dynamic detection of biomolecular interaction is as follows:

[0034] 1) Dissolving the PDMA material first to prepare a polymer material solution, the concentration of the polymer material solution is 20wt%;

[0035] 2) immersing the chip body in the polymer material solution, so that the polymer material solution fills the gaps generated by the patterning of the surface metal film layer on the chip body, and the immersion time is 1 h;

[0036] 3) After immersion, heat at 300°C for 1 hour to rapidly solidify the polymer material on the surface of the chip to form a film. The optical properties of the cured film are similar to those of the optical coupler. The optical properties referred to here refer to the refractive index and absorption coefficient. Wait.

Embodiment 2

[0038] The preparation method of the detection chip for dynamic detection of biomolecular interaction is as follows:

[0039]1) First mix and dissolve the PVA material and the photocuring agent to prepare a polymer material solution, and the concentration of the polymer material solution is 25wt%;

[0040] 2) adding the polymer material solution dropwise to the surface of the chip body, so that the polymer material solution is filled into the gaps generated by the patterning of the surface metal film layer on the chip body, and the standing time after dropping is 1h;

[0041] 3) Then irradiate with ultraviolet light to quickly solidify the polymer material on the surface of the chip to form a film. The optical properties of the cured film are similar to those of the optical coupler. The optical properties referred to here refer to the refractive index and absorption coefficient.

Embodiment 3

[0043] The preparation method of the detection chip for dynamic detection of biomolecular interaction is as follows:

[0044] 1) dissolving the chitosan-based gel material first to prepare a polymer material solution, the concentration of the polymer material solution being 30wt%;

[0045] 2) adding the polymer material solution dropwise to the surface of the chip body, so that the polymer material solution is filled into the gaps generated by the patterning of the surface metal film layer on the chip body, and the standing time after dropping is 1h;

[0046] 3) Then add a cross-linking agent, and the cross-linking time is 2 hours, so that the surface of the chip can quickly solidify the polymer material to form a film. The optical properties of the cured film are relatively similar to those of the optical coupler. The optical properties referred to here refer to Refractive index, absorption coefficient, etc.

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PUM

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Abstract

The invention provides a detecting chip used for dynamically detecting the interactive action of biomolecules. The detecting chip is mainly composed of a chip body and solidified filling materials. Gaps which are generated by patterning of a surface metal membrane layer on the chip body are filled with the solidified filling materials. A preparation method of the detecting chip mainly includes the following steps that A, a macromolecular material solution is prepared and fused with the chip body so that the macromolecular solution can seep into the gaps which are generated by patterning of the surface metal membrane layer on the chip body; B, the chip body after treatment in the step A is subjected to solidifying treatment so that the macromolecular material solution can be solidified into the solidified filling materials. The detecting chip guarantees the uniformity of chips, and also avoids that in the prior art, when matching fluid is dropwise added, due to the difference of dropwise-adding positions and methods, drop sizes and foams, detecting structures make errors.

Description

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Claims

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Application Information

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Owner 苏州奥普特克自动化科技有限公司
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