Kaki calyx extract and application thereof
A technique for extracting and persimmon stalks, applied in the field of natural product chemistry
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Embodiment 1
[0016] Embodiment 1: the concentration of extraction solvent (ethanol) is selected
[0017] 1. Take 10 g of the dried and pulverized powder of persimmon stems and leaves, soak it with 100 ml of pure water at room temperature and ultrasonically extract it twice, each time for 60 minutes (500 W), combine the filtrate after filtration, recover the solvent under reduced pressure until dry, and obtain crude persimmon pedicles. Extract; take the obtained persimmon pedicle crude extract and prepare a 0.05mg / ml solution for later use.
[0018] 2. Take 10 g of the dried and pulverized powder of persimmon stalks and leaves, soak it with 100 ml of ethanol solution with a volume concentration of 30% at room temperature and extract it ultrasonically twice, each time for 60 min (500 W). After filtering, combine the filtrates, and recover the solvent under reduced pressure to dryness. , to obtain the crude extract of persimmon pedicle; take the obtained crude persimmon pedicle extract and pr...
Embodiment 2
[0033] Embodiment 2: the selection of solid-liquid ratio
[0034] 1. Take 10 g of the dried and pulverized powder of persimmon stalks and leaves, soak it with 80 ml of ethanol solution with a volume concentration of 60% at room temperature and extract it twice with ultrasound, each time for 60 min (500 W). After filtering, combine the filtrates, and recover the solvent under reduced pressure to dryness. , to obtain the crude extract of persimmon pedicle; take the obtained crude persimmon pedicle extract and prepare a 0.05mg / ml solution for later use.
[0035] 2. Take 10 g of the dried and pulverized powder of persimmon stalks and leaves, soak it with 100 ml of ethanol solution with a volume concentration of 60% at room temperature and extract it twice with ultrasound, each time for 60 min (500 W). After filtering, combine the filtrates and recover the solvent under reduced pressure to dryness. , to obtain the crude extract of persimmon pedicle; take the obtained crude persimmo...
Embodiment 3
[0042] Embodiment 3: the preparation of persimmon pedicle extract
[0043] The preparation of crude extract of persimmon stalks: take 10 g of dried and pulverized powder of persimmon stalks and leaves, immerse at room temperature with 120 ml of ethanol solution with a volume concentration of 60%, and ultrasonically extract 2 times, each time for 60 min (500 W), filter and combine the filtrates, The solvent was recovered under reduced pressure to dryness, and the crude extract of persimmon stalks was obtained for efficacy research.
[0044] Preparation of persimmon pedicle activity-enriched extract: Get 100 g of dried and pulverized powder of persimmon pedicle stem and leaf, soak it with 1200 ml of ethanol solution with a volume concentration of 60% at room temperature and extract it twice by ultrasonic, each time for 60 min (500 W), and combine the filtrate ( About 2200ml), the solvent is recovered under reduced pressure until there is no alcohol smell, passed through D101 res...
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