Method for analyzing urine exosome miRNA

An analysis method and exosome technology, applied in the field of biomedicine, can solve problems such as large error and complicated operation, and achieve the effects of convenient operation, reliable detection data, and reasonable and feasible design.

Pending Publication Date: 2017-02-15
THE SECOND AFFILIATED HOSPITAL TO NANCHANG UNIV
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Problems solved by technology

[0004] In view of the above-mentioned deficiencies in the prior art, the present invention solves the problems of cumbersome operation and relatively large errors i

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  • Method for analyzing urine exosome miRNA
  • Method for analyzing urine exosome miRNA
  • Method for analyzing urine exosome miRNA

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Embodiment Construction

[0027] The technical solution of the present invention will be further specifically described below through specific embodiments and in conjunction with the accompanying drawings. Unless otherwise specified, the technical means and operating methods used in the following examples are conventional means and methods well known to those skilled in the art, and the raw materials used are all commercially available commodities.

[0028] 1. Collection of urine samples

[0029] In this example, 3 cases of clinically diagnosed patients with IgA nephropathy and healthy persons with similar ages were selected, and 120 ml of morning urine were collected respectively as the IgA nephropathy group and healthy control group for subsequent experimental operations. The 6 specimens were all from the Second Affiliated Hospital of Nanchang University. The clinical diagnosis was confirmed. Patients with other glomerular diseases and complications were excluded. All IgA nephropathy urine specimens ...

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Abstract

The invention provides a method for analyzing urine exosome miRNA, comprising: collecting a urine sample, extracting exosomes and their total RNA, establishing a cDNA library, performing Illumina sequencing, and processing and annotating the sequencing results to obtain expression information of sample miRNA expression quantity; urine sample exosome miRNA is analyzed by high-throughput sequencing technology to acquire expression information of miRNA; a database for IgA nephrotic patient and a healthy control patient is established, differentially expressed miRNA is deeply studied through target gene function prediction, the deep study on the differentially expressed miRNA helps further illustrate the pathogenesis of IgA nephropathy, and novel method and theoretical basis is provided for the diagnosis of IgA nephropathy. The method is reasonable and feasible in design, a noninvasive precise differential expression miRNA method can be effectively established, and novel ideas are provided for clinical diagnosis and treatment.

Description

technical field [0001] The invention relates to biomedicine, in particular to a method for analyzing urine exosome miRNA. Background technique [0002] Urine is a commonly used specimen in clinical testing. Because it can be obtained through non-invasive operations and easy to collect in large quantities, it has become an important source of specimens for the diagnosis of kidney diseases. The current research on renal disease biomarkers in urine mainly focuses on proteomics and metabolomics. However, these protein and metabolite biomarkers are easily interfered by other factors and require high specimen preservation. [0003] Exosomes were once considered meaningless cellular "dust", but subsequent studies have confirmed that they are important carriers of biological information. Exosomes contain many biologically active molecules including proteins (growth factors and cytokines, etc.), mRNA and microRNA, which provide a material basis for them to become disease diagnostic ...

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6869C12Q1/6883C12Q2600/158C12Q2600/178C12Q2531/113C12Q2535/122C12Q2539/113
Inventor 黄波王小中邹叶青刘静徐颜美闵清华
Owner THE SECOND AFFILIATED HOSPITAL TO NANCHANG UNIV
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