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A kind of Gastrodia elata symbiotic Armillaria strain and preparation method thereof

The technology of Armillaria and Gastrodia elata is applied in the field of Armillaria symbiotica and its preparation field, and can solve the problems such as no species of Armillaria symbiosis with Gastrodia elata.

Active Publication Date: 2020-04-10
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] So far, there is no report on the symbiotic Armillaria genus Gastrodia genus Armillaria cepistipes Velen., the strain preservation registration number is: CGMCC No.11902, and its preparation method and application in the prior art

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] 1. Preparation of test tube strains:

[0039] 1) Select the source of Armillaria mellea in the field (collect the source material of Armillaria mellea from different parts of the Zhaotong gastrodia elata production area) as the introduction bacteria, mix it with broad-leaved branches with a diameter of 50-80mm, and plant it in the pond under the forest as the fungus material. - In August, spread the Gastrodia elata seeds mixed with the germination bacteria evenly on the fungus in the pond, and cover the fresh leaves of broad-leaved trees and sand for cultivation. After 16-24 months, choose the gastrodia elata that grows in the pond with a large number and good shape Bacteria culture strains.

[0040] 2) Separation and purification of bacterial cords: select a few segments of bacterial cords that are clustered, suitable in thickness, robust in growth, and white at the top, and placed in a petri dish on the cultivated bacterial material, and inoculated on the slant medium...

Embodiment 2

[0045] 1. Preparation of test tube strains:

[0046] 1) Select the source of Armillaria mellea in the field: widely collect the source materials of Armillaria mellea from different areas in Zhaotong Wugastrodia elata production area as the introduction bacteria, mix them with broad-leaved branches with a diameter of 50-80mm and plant them in forest ponds as fungus materials, and in the following year Gastrodia elata seeds are naturally matured in July-August, and then the gastrodia elata seeds mixed with germination bacteria are evenly spread on the fungus in the pond, and the fresh leaves of broad-leaved trees and sand are covered for cultivation. After 16-24 months, the gastrodia elata in the pond is selected. Bacteria source materials with a large number of growth and good shape are used to cultivate bacterial strands.

[0047] 2) Separation and purification of bacterial strands: select several sections of light-colored and robust bacterial strands from the cultivated bacte...

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Abstract

The invention provides an Armillaria cepistipes Velen. strain and a method for preparing the same. A systematic name of the strain is Armillaria cepistipes Velen., and a preservation registration number of the strain is CGMCC No.11902. The method includes strain selection, separation and purification, identification and cultivation. The Armillaria cepistipes Velen. strain and the method have the advantages that risks of low gastrodia elata Bl, yield or zero-output 'empty pond' phenomena due to inappropriate strain selective breeding can be reduced; the strain and gastrodia elata BL. f. glauca S. Chow are good in affinity, and accordingly the original quality of the gastrodia elata BL. f. glauca S. Chow in main gastrodia elata BL. f. glauca S. Chow producing regions under large-scale artificial cultivation conditions can be guaranteed.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a symbiotic Armillaria Gastrodia elata and a preparation method thereof. Background technique [0002] Armillaria fungi (Armillaria), commonly known as hazel mushrooms and corn fungi, belong to the kingdom Fungi, the subphylum Basidiomycotina, the class Hymenomyeetes, the order Agaricales, and the family Physalacriaceae. There are about 40 species of this genus of fungi in the world, and they are widely distributed in China. They are distributed in central China, southwest and northeast regions. Armillaria is an important symbiotic fungus of Gastrodia elata Bl., a precious traditional Chinese medicine. Gastrodia elata Bl. requires 95% of the growth period of Gastrodia elata to provide nutrients in the form of root-like cords. Studies have shown that Gastrodia elata is grown with different sources of Armillaria elata, and the yield and quality of Gastrodia elata are diffe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G18/00
CPCA01G18/00
Inventor 郭婷杨祝良
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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