Method for controlling haematococcus pluvialis pests and diseases by using photocatalyst

Abstract

The invention provides a method for controlling haematococcus pluvialis pests and diseases by using a photocatalyst and belongs to the biotechnology field. The photocatalyst is added to a culture medium, the growth of invading harmful organisms can be effectively inhibited by using the characteristic that reactive oxygen is produced under the photocatalysis action of the photocatalyst under a certain light condition, meanwhile, production of reactive oxygen can promote the haematococcus pluvialis to turn into chlamydospores from swarm cells, and the capacity of the haematococcus pluvialis for resisting the harmful organisms is further improved. The photocatalyst is used as a catalyst and cannot be consumed in the culture process, and the pest and disease control effect can be continued to the end of culture all the time. Besides, on the basis of the difference between the cell particle size and specific gravity of a photocatalyst material and haematococcus pluvialis, the photocatalyst material can be recycled through simple filtration, sedimentation and other methods. The method is simple to operate, low in cost, safe, efficient and suitable for mass production and application.

Description

technical field [0001] The invention relates to the field of biotechnology, and in particular provides a method for controlling microalgae diseases and insect pests by using a photocatalyst. Background technique [0002] Haematococcus pluvialis is a freshwater single-celled green alga belonging to Chlorophyta, Chlorophyceae, Volvox, Haematococcus, and Haematococcus. Haematococcus pluvialis is called Haematococcus pluvialis or Haematococcus pluvialis because it can accumulate a large amount of astaxanthin and make the algae appear red. It is considered to be the best biological platform for producing astaxanthin in nature. The natural astaxanthin produced by Haematococcus pluvialis has good quality and strong antioxidant activity. However, the current global production of Haematococcus pluvialis is still very low, and the immaturity of large-scale cultivation technology is the main limiting factor. For example, related technologies such as algae breeding, photobioreactor des...

AI Technical Summary

Problems solved by technology

In the process of large-scale cultivation, the invasion of harmful organisms will bring serious harm, even if it is a closed photobioreactor, it is difficult to avoid

Once harmful organisms appear, such as protozoa, miscellaneous algae, bacteria, chytrid, etc., it will affect the growth of the target algae species, and in severe cases, it will lead to no harvest

[0003] In recent years, there have been many research reports on the control of microalgae pests and diseases, but they are mainly broad-spectrum methods, so the selectivity is not enough

The prevention and control methods of microalgae pests and diseases mainly fall into the following categories: pretreatment methods for microalgae cultivation, such as ozone, ultraviolet light, sodium hypochlorite, physical filtration, etc. These methods are not selective and will cause damage to microalgae, so they are only suitable for cultivation Pre-treatment, but not for the pests and diseases that appear during the cultivation process; physical methods during the cultivation process, such as the use of microporous filtration and nylon mesh isolation, etc., this method can remove large algae-eating animals, but for small individuals Algae rotifers and their eggs are difficult to eradicate, and the filtration speed is relatively slow, so the application in large-scale production is limited; the chemical methods in the cultivation process mainly use drugs such as formaldehyde, ammonia, hydrogen peroxide and sodium hypochlorite, and Lowering the pH and other methods, but these methods are not specific, and the treatment process will cause serious harm to microalgae

In addition, there are control methods of adding heavy metals, organophosphorus, and carbamate pesticides, but these methods only consider the control of pests and diseases alone, and do not consider the impact on the growth and reproduction of microalgae and product quality.