A kind of method utilizing soy sauce filter residue to prepare d-glucosylceramide

Abstract

The invention relates to a method for preparing D-glucosylceramide by using a soy sauce filter residue, and is characterized in that the method comprises the following steps: activating strains: A, respectively inoculating 100 mL of an activation culture medium with an sphingomonas strain and a yarrowia lipolytica strain; B, carrying out aerobic fermentation culture of the sphingomonas strain activated culture liquid P and the yarrowia lipolytica strain activated culture liquid Q obtained in the step A for 36-72 h, to obtain a composite strain fermentation liquid; C, mixing the composite strain fermentation liquid with the soy sauce filter residue, adding the mixture into a compound fermentation tank, carrying out static culture, and culturing for 48-96 h at the temperature of 25-30 DEG C, to obtain a strain cell-rich filter residue; and carrying out high pressure homogenization for 2 times, to obtain a wall-broken cell homogenate; and D, mixing the wall-broken cell homogenate with an ethanol solution, filtering to obtain a precipitate, and thus obtaining a D-glucosylceramide extract. Through rational use of the soy sauce filter residue, the waste can be turned into treasure, soy sauce industries are facilitated to improve economic benefits, and the resource utilization of the soy sauce filter residue and the development of high-value by-products are greatly improved.

Description

field of invention

[0001] The invention belongs to the technical field of biological fermentation, in particular to a method for preparing D-glucosylceramide by using soy sauce filter residue. Background technique

[0002] my country is a big producer of soy sauce, and its current annual output has reached more than 5 million tons, accounting for more than 60% of the world's total annual output. With the rapid development of soy sauce industry, the treatment of soy sauce waste residue has become an urgent problem for soy sauce production enterprises. It is estimated that a soy sauce factory with an annual output of 500 tons can produce 150 tons of wet soy sauce residue. Traditionally, soy sauce filter residue has no economic value and is often discarded as waste. In fact, soy sauce filter residue is a "waste" worth developing. Soy sauce filter residue is rich in crude protein, crude fiber, crude fat and other active components, and is an ideal high-value conversion raw mat...

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