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DCP quick separation detection kit

A detection kit and rapid technology, applied in the field of medical devices and in vitro diagnostics, can solve the problems of limited monoclonal antibody recognition epitopes, low detection sensitivity, and inability to capture DCP

Inactive Publication Date: 2017-03-01
BEIJING HOTGEN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the current research, there have been some different monoclonal antibodies for the diagnosis of HCC, such as MU3 and 3C10, but due to the limited recognition epitopes of monoclonal antibodies, they cannot capture all DCPs, so the detection sensitivity is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] This embodiment provides a DCP rapid separation and detection kit, and provides a preparation method for producing a preferred anti-DCP monoclonal antibody for preparing the kit.

[0056] 1. Preparation of anti-DCP monoclonal antibody:

[0057] The preparation of anti-DCP monoclonal antibodies in this example can be the first antibody, the second antibody or the combination of the first antibody and the second antibody produced by hybridoma cells 4D09 and 3H08, or other commercial anti-DCP monoclonal antibodies Antibody.

[0058] In order to better explain the present invention, the preparation of the anti-DCP monoclonal antibody is preferably the preparation of the first antibody and the second antibody composition; this preparation method is only for better explanation of the present invention, not for the preparation and use of the anti-DCP monoclonal antibody. limitation factor.

[0059] Take hybridoma cells 4D09 and 3H08 (preserved in the General Microbiology Cen...

Embodiment 2

[0086] The invention provides a DCP rapid separation detection kit and its application, which is mainly used for accurate and quantitative detection of DCP content in human serum samples, and the detection results of serum samples are used for primary hepatocellular carcinoma (HCC) and chronic liver disease Differential diagnosis of benign hepatic space-occupying lesions.

[0087] The following detection steps are mainly used to better explain the use and application of the present invention, and are not used as limiting conditions for the application of the present invention.

[0088] The main detection steps are as follows:

[0089] 1. The compatible instrument for Example 2 is: Maglumi series automatic immune analyzer (MQ60 type) produced by Beijing Rejing Biotechnology Co., Ltd.

[0090] 2. MQ60 detection steps:

[0091] (1) Reagent preparation

[0092] a) The kit should be equilibrated to room temperature when it is taken out from the refrigerated environment before us...

Embodiment 3

[0110] A DCP rapid separation detection kit and application thereof of the present invention are mainly used for accurate and quantitative detection of DCP content in human serum samples, and the detection results of serum samples are used for primary hepatocellular carcinoma (HCC) and chronic liver disease and benign Differential diagnosis of hepatic space-occupying lesions.

[0111] The following detection steps are mainly used to better explain the use and application of the present invention, and are not used as limiting conditions for the application of the present invention.

[0112] The main performance indicators are as follows:

[0113] 1. The compatible instrument for Example 2 is: Maglumi series automatic immune analyzer (MQ60 type) produced by Beijing Rejing Biotechnology Co., Ltd.

[0114] 2. Minimum detection limit:

[0115] The lowest detection limit of DCP means that the DCP content can be distinguished from the point where the DCP content is zero. The high-...

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Abstract

The invention provides a DCP quick separation detection kit, by which content of abnormal prothrombin (DCP) in a human serum sample can be quickly, accurately and quantitatively metered in vitro, so that the kit is mainly used in clinical identification and diagnosis on primary hepatocellular carcinoma (HCC), chronic liver diseases and benign hepatic space-occupying lesions.

Description

technical field [0001] The invention belongs to the sub-fields of medical devices and in vitro diagnosis. Specifically, the present invention relates to a DCP rapid separation and detection kit, and a monoclonal antibody and its hybridoma cells preferably used for the preparation of the abnormal prothrombin (DCP) rapid separation and detection kit. Background technique [0002] Hepatocellular carcinoma (HCC) is a common malignant tumor disease in the world, and its incidence is increasing year by year, and now it ranks fourth in the incidence of all tumors in the world. HCC has a high mortality rate and ranks the third leading cause of cancer death in the world. About 250,000 patients worldwide die of liver cancer cells every year. The pathogenesis of HCC is still unclear, and the lack of effective early diagnosis techniques and methods is an important reason for the high mortality of HCC. Therefore, early detection and early treatment are the keys to the survival of HCC p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543G01N33/573G01N33/577
CPCG01N33/573G01N33/54326G01N33/57438G01N33/577G01N2800/085
Inventor 高琦李伯安乔雍郄霜马海雪刘飞李辉李艳召余韶华
Owner BEIJING HOTGEN BIOTECH CO LTD
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