A virus-free breeding method and virus-free culture medium for cranberry

A medium and basal medium technology, applied in horticultural methods, botanical equipment and methods, plant cells, etc., can solve the problem of different sensitivities and resistances, different detoxification methods and detoxification effects, and bilberry detoxification. The virus has not been reported and other problems, to achieve the effect of improving the detoxification rate, solving the incomplete detoxification, and reducing the probability of the germ infecting the buds

Inactive Publication Date: 2018-07-20
FORESTRY RES INST OF HEILONGJIANG PROVINCE +1
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AI Technical Summary

Problems solved by technology

However, since different plants have different sensitivities and resistances to the same infection virus, and different viruses are selective for the infected host plants, and the callus often undergoes some mutations after multiple subcultures, resulting in different host plants and different viruses. The detoxification methods and detoxification effects are not the same
Although potato, sweet potato, garlic, banana, citrus, apple, grape, lily, strawberry, petunia, carnation, rose, chrysanthemum, peony, taro, camellia, sugarcane, strawberry, etc. Widely used in China, but detoxification of lingonberry has not been reported

Method used

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  • A virus-free breeding method and virus-free culture medium for cranberry

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] Take the dormant branches of lingonberry for hydroponics to accelerate germination, cut off the dormant buds when they grow to 2cm, and sterilize them on the ultra-clean workbench. Wash with 70% ethanol for 60 seconds, rinse once with sterile water, soak in 0.1% mercuric chloride for 3 minutes, rinse with sterile water for 5 times, and blot the surface moisture with sterile filter paper. Cut the shoot tip 0.3-0.8cm and inoculate it into an additional trans-zeatin 0.8mg / L, add sucrose 18g / L, agar 5.0g / L, pH 5.5 on the improved WPM medium for aseptic culture, the improved WPM Medium composition and dosage: 400mg / LNH 4 NO 3 , 202mg / LKNO 3 , 198mg / L (NH 4 )2SO 4 、408mg / LKH 2 PO 4 , 370mg / LMgSO 4 .7H 2 O, 708mg / L Ca(NO 3 ) 2 .4H 2 O, 6.2 mg / L H 3 BO 3 , 22.3mg / L MnSO 4 4H 2 O, 8.6mg / LZnSO 4 ·7H 2 O, 0.25mg / LNa 2 MoO 4 2H 2 O, 0.25mg / L CuSO 4 ·5H 2 O, 27.8mg / L FeSO 4 ·7H 2 O, 37.3mg / L Na 2-EDTA, 100mg / L inositol, 1mg / L thiamine hydrochloride, 0.5mg / L ...

Embodiment 2

[0070] Take the dormant branches of lingonberry for hydroponics to accelerate germination, cut off the dormant buds when they grow to 2cm, and sterilize them on the ultra-clean workbench. Wash with 70% ethanol for 60 seconds, rinse once with sterile water, soak in 0.1% mercuric chloride for 3 minutes, rinse with sterile water for 5 times, and blot the surface moisture with sterile filter paper. Cut the stem tip 0.8cm and inoculate it to additional trans-zeatin 1.2mg / L, add sucrose 23g / L, agar 6.0g / L, and carry out aseptic culture on the improved WPM medium that pH is 5.4, and the improved WPM medium Ingredients and dosage: 400mg / LNH 4 NO 3 , 202mg / LKNO 3 , 198mg / L (NH 4 )2SO 4 、408mg / LKH 2 PO 4 , 370mg / LMgSO 4 .7H 2 O, 708mg / L Ca(NO 3 ) 2 .4H 2 O, 6.2 mg / L H 3 BO 3 , 22.3mg / L MnSO 4 4H 2 O, 8.6mg / LZnSO 4 ·7H 2 O, 0.25mg / LNa 2 MoO 4 2H 2 O, 0.25mg / L CuSO 4 ·5H 2 O, 27.8mg / LFeSO 4 ·7H 2 O, 37.3mg / LNa 2 -EDTA, 100mg / L inositol, 1mg / L thiamine hydrochlorid...

Embodiment 3

[0072] Take the dormant branches of lingonberry for hydroponics to accelerate germination, cut off the dormant buds when they grow to 2cm, and sterilize them on the ultra-clean workbench. Wash with 70% ethanol for 60 seconds, rinse once with sterile water, soak in 0.1% mercuric chloride for 3 minutes, rinse with sterile water for 5 times, and blot the surface moisture with sterile filter paper. Cut the shoot tip 0.5cm and inoculate it to additional trans-zeatin 1.0mg / L, add sucrose 20g / L, agar 5.6g / L, and carry out aseptic culture on the improved WPM medium that pH is 5.4, and the improved WPM medium Ingredients and dosage: 400mg / LNH 4 NO 3 , 202mg / LKNO 3 , 198mg / L (NH 4 )2SO 4 、408mg / LKH 2 PO 4 , 370mg / LMgSO 4 .7H 2 O, 708mg / L Ca(NO 3 ) 2 .4H 2 O, 6.2 mg / L H 3 BO 3 , 22.3mg / L MnSO 4 4H 2 O, 8.6mg / LZnSO 4 ·7H 2 O, 0.25mg / LNa 2 MoO 4 2H 2 O, 0.25mg / L CuSO 4 ·5H 2 O, 27.8mg / L FeSO 4 ·7H 2 O, 37.3mg / L Na 2 -EDTA, 100mg / L inositol, 1mg / L thiamine hydrochlo...

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Abstract

The invention provides a cowberry detoxification breeding method. The cowberry detoxification breeding method comprises the following steps: accelerating germination of cowberry resting shoots, shearing off a bud when the length of the bud reaches 1-3 cm and carrying out sterilizing treatment; cutting off 0.3-0.8 cm of a stem tip from the sterilized bud, and inoculating the stem tip on a foundational medium, and culturing under the conditions that the temperature is 25-28 DEG C, the illumination intensity is 1,800-2,200 Lx, and the illumination time is 12-16 h / d; after the stem tip grows until the length of the stem tip reaches 1.5-2.0 cm, cutting off 0.03-0.05 cm of a stem tip in a sterile environment and inoculating the stem tip to a detoxification medium, carrying out dark culture for 5-9 days at the temperature of 22-25 DEG C, and continuing culturing in an environment in which the temperature is 23-26 DEG C, the illumination intensity is 1,800-2,200 Lx and the illumination time is 12-16 h / d; and inoculating a detoxification seedling to a medium, carrying out rapid multiplication culture, and then carrying out seedling hardening and rooting after the length of the seedling reaches 2.0-3.0 cm.

Description

technical field [0001] The invention relates to the technical field of plant virus-free culture and rapid propagation, in particular to a method for breeding virus-free cranberry seedlings and a virus-free culture medium. Background technique [0002] In actual production, many asexually propagated plants can be infected by viruses. Vaccinium is propagated by creeping rhizomes formed by the mother plant, and seedlings are susceptible to viruses during growth. Viruses can cause devastating damage to parasitic bilberry, resulting in Substantial reduction in production, or even the death of the whole plant, will also lead to the degradation of the species and the deterioration of the quality. It is the basic requirement of actual production and breeding to keep the excellent characteristics of varieties well. Therefore, it is very important for cranberry production to seek a treatment method that can not only maintain the excellent characteristics of varieties but also get rid ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01H4/00
CPCA01H4/001A01H4/008
Inventor 李艳霞李桂君刘建明刘忠玲王洪梅田新华
Owner FORESTRY RES INST OF HEILONGJIANG PROVINCE
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