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Construction method of oral submucous fibrosis mouse model

An oral mucosa, mouse model technology, applied in the medical field, can solve the problem of lack of models, and achieve the effect of improving accuracy, ideal effect, and diversifying disease sites.

Active Publication Date: 2017-03-22
海南省人民医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If you want to explore the occurrence and development of OSF and the outcome of the disease, none of the existing models are really feasible and reliable

Method used

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  • Construction method of oral submucous fibrosis mouse model
  • Construction method of oral submucous fibrosis mouse model
  • Construction method of oral submucous fibrosis mouse model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] (1) Animal selection and grouping: 40 8-week-old male Balb / c mice were taken as experimental mice, and randomly divided into experimental group and control group;

[0024] The conditions of the experimental mice are: the experimental mice are: 8-week-old male Balb / c mice, SPF grade, body weight (18.52±1.15) g, at room temperature 18~25°C, humidity 30%~50%, Standard feed feeding, feeding under 24h light and dark cycle conditions;

[0025] (2) Preparation of arecoline drinking water preparation: the arecoline (arecoline) preserved at normal temperature was prepared with tap water to a concentration of 2000 mg / L and placed in the drinking water bottles of the mice in the experimental group to drink freely. Mice in the control group drank tap water freely;

[0026] (3) Feeding and killing of animals: the experimental group was fed with arecoline drinking water for 20 weeks, and the control group was fed with tap water at the same time, and 4 mice in each group were randoml...

Embodiment 2

[0040] (1) Animal selection and grouping: 40 8-week-old male Balb / c mice were taken as experimental mice, and randomly divided into experimental group and control group;

[0041] The conditions of the experimental mice are: the experimental mice are: 8-week-old male Balb / c mice, SPF grade, body weight (18.52±1.15) g, at room temperature 18~25°C, humidity 30%~50%, Standard feed feeding, feeding under 24h light and dark cycle conditions;

[0042] (2) Preparation of arecoline drinking water preparation: the arecoline (arecoline) preserved at normal temperature was prepared with tap water to a concentration of 500 mg / L and placed in the drinking water bottles of the mice in the experimental group to drink freely. Mice in the control group drank tap water freely;

[0043] (3) Feeding and killing of animals: the experimental group was fed with arecoline drinking water for 20 weeks, and the control group was fed with tap water at the same time, and 4 mice in each group were randomly...

Embodiment 3

[0057] (1) Animal selection and grouping: 40 8-week-old male Balb / c mice were taken as experimental mice, and randomly divided into experimental group and control group;

[0058] The conditions of the experimental mice are: the experimental mice are: 8-week-old male Balb / c mice, SPF grade, body weight (18.52±1.15) g, at room temperature 18~25°C, humidity 30%~50%, Standard feed feeding, feeding under 24h light and dark cycle conditions;

[0059] (2) Preparation of arecoline drinking water preparation: the arecoline (arecoline) preserved at normal temperature was prepared with tap water to a concentration of 1000 mg / L and placed in the drinking water bottles of the mice in the experimental group to drink freely. Mice in the control group drank tap water freely;

[0060] (3) Feeding and killing of animals: the experimental group was fed with arecoline drinking water for 20 weeks, and the control group was fed with tap water at the same time, and 4 mice in each group were randoml...

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Abstract

The invention discloses a construction method of an oral submucous fibrosis (OSF) mouse model. The construction method comprises the following steps: (1) selection and grouping of animals; (2) preparation of an arecoline drinking agent; (3) feeding and killing of the animals; (4) experimental observation; (5) construction of the mouse OSF model. According to the construction method of the OSF mouse model, a Balb / c mouse is fed by the arecoline drinking agent with the concentration of 500 to 2,000 mg / L for 20 weeks, and the obtained mouse OSF model is highly similar to human OSF; the animal model is high in morbidity (100 percent) and has varieties of pathogenic sites; mucous membranes at all parts in an oral cavity can be attacked; an induction method is simple and natural; the mouse drinks water freely, so that influence caused by physical stimulation and stimulation of other chemical medicines is prevented; the effect is ideal, and the induction time is short.

Description

technical field [0001] The invention relates to the medical field, in particular to a method for constructing a mouse model of oral submucosal fibrosis through the administration method and the administration dosage process. Background technique [0002] Oral submucosal fibrosis (OSF) is a chronic, insidious, malignant disease of the oral mucosa that can occur in any part of the oral mucosa. Follow-up studies have shown that the incidence of oral submucosal fibrosis increases year by year with the commercialization of betel nut, but there is no good clinical treatment for OSF at present. Therefore, the research of OSF model has been paid attention to. There are very few reports on animal models of OSF at home and abroad, which greatly restricts the further in-depth study of OSF. In 1960, Sirsat et al. used 2% capsaicin to surface-coat the palatal mucosa of rats to induce OSF-like lesions. However, its pathological manifestations are not typical, and there are no obvious c...

Claims

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Application Information

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IPC IPC(8): A61K31/455A61P1/02
CPCA61K31/455
Inventor 王涛温琦涛于大海王振瑞梁翠薇施强
Owner 海南省人民医院
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